Vážení zákazníci,přichází JARNÍ AKCE 2026, během které můžete využít zvýhodněné ceny na široký sortiment spotřebního materiálu pro vaši laboratoř.<h4>Nabízíme vše, co potřebujete pro každodenní provoz i náročné analýzy:</h4><ul><li>kolony pro HPLC, UHPLC, BioLC</li><li>kolony pro GC a IC</li><li>spotřební materiál pro přípravu vzorků</li><li>drobné laboratorní vybavení</li></ul>Kvalitní spotřební materiál je základem spolehlivých výsledků. Využijte proto tuto příležitost a doplňte své zásoby za výhodnějších podmínek.<blockquote>Akce je platná na území ČR od 1. 4. do 31. 5. 2026.Kontaktujte nás jednoduše na e-mailu: chromatografie@pragolab.cz.</blockquote><h4>−30 % HPLC kolony</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products/423">Hypersil GOLD</a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products?category=48&amp;manufacturerItems=3&amp;search=accucore">Accucore</a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=Syncronis">Syncronis</a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=Hypercarb">Hypercarb</a></li></ul><h4>−20 % BioLC kolony a kolony pro speciální aplikace</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=MabPac">MabPac</a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=ProPac">ProPac</a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=PepMap">PepMap</a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=EasySpray">EasySpray</a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=Acclaim">Acclaim</a></li></ul><h4>−20 % GC kolony</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/products?category=7&amp;distributorItems=42&amp;search=TraceGOLD">TraceGOLD</a> — <a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/products?category=7&amp;distributorItems=42&amp;search=Trace">Trace GC</a> — <a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/products?category=7&amp;distributorItems=42&amp;search=TracePLOT">Trace PLOT</a></li></ul><h4>−25 % IC kolony a spotřební materiál</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?instrumentationItems=28-34&amp;mainauthorItems=3&amp;search=columns">Kolony</a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?instrumentationItems=28&amp;mainauthorItems=3&amp;search=EGC">EGC</a> — supresory — vialky</li></ul><h4>−25 % <a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/Pragolab_vialka_f386946736/Pragolab_vialka_.pdf">Vialky Pragolab</a></h4><ul><li>Vialky — septa — kity</li></ul><h3>Přehled nejnovější literatury z oblasti spotřebního matriálu od Thermo Scientific v knihovně <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?from=2025&amp;instrumentationItems=20&amp;manufacturerItems=2&amp;to=2026">LabRulezLCMS</a></h3><ul><li>Instrument and LC column migration for the purification and analysis of synthetic oligonucleotides (Aplikace | 2025)</li><li>Evolution of Ion-Exchange columns for biologics characterization (Prezentace | 2025)</li><li>Advancing Low flow LC/MS for single cell proteomics with variable flow and 50 cm microfabricated pillar array columns (Prezentace | 2025)</li><li>Wide-ranging polynucleotide separation capabilities using Reversed Phase particles with variable pore geometry (Postery | 2025)</li><li>Evaluation of batch-to-batch consistency of reversed phase HPLC columns for long-term method validation (Postery | 2025)</li><li>Efficient tandem capillary flow LC-MS with short μPAC columns and a single ionization source (Postery | 2025)</li><li>At the intersection between chromatographic performance, ESI efficiency and instrument productivity: nano to capillary flow LC/MS on long μPAC Columns (Postery | 2025)</li><li>Achieving consistent SEC performance through the use of 3 μm, 550 Å monodisperse media in novel bioinert column hardware (Postery | 2025)</li><li>Improved accuracy and identification in label-free quantitative proteomics with the OptiSpray Ion Source and separation cartridge with integrated replaceable emitters (Postery | 2025)</li><li>Accucore columns Resolve all your impurities without feeling the pressure (Ostatní | 2025)</li><li>SureSTART Vial Quick Select Guide (Ostatní | 2025)</li><li>Sensitive cationic lipids impurities analysis with quantitation by charged aerosol detection and simultaneous mass confirmation by MS (Aplikace | 2025)</li><li>Thermo Scientific Viper TQ UHPLC Fittings (Brožury a specifikace | 2025)</li><li>HPLC vials and well plates - Every drop counts (Brožury a specifikace | 2025)</li></ul><h3>Vybrané nejnovější <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinars?companyId=42">webináře</a> Thermo Scientific z oblasti spotřebního materiálu</h3><h5>1. Mastering Single Cell Proteomics: Daily Excellence with μPAC Neo Plus Trap-and-Elute Workflow</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/5789">ZÁZNAM</a> | Proběhlo Út, 27.1.2026</li></ul>Zjistěte, jak dosáhnout robustní, vysoce citlivé proteomiky jednotlivých buněk pomocí systému μPAC Neo Plus trap-and-elute LC-MS se systémy Vanquish Neo a Orbitrap.<h5>2. Monodisperse inert HPLC columns for large molecule analysis in biopharma</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/5729">ZÁZNAM</a> | Proběhlo Út, 9.12.2025</li></ul>Připojte se k nám na zajímavém webináři o pokročilých chromatografických metodách pro analýzu velkých biomolekul.<h5>3. Reduce analyte loss in LC workflows: The role of vial and surface chemistry</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/5675">ZÁZNAM</a> | Proběhlo Po, 17.11.2025</li></ul>Objevte strategie pro zvýšení výtěžnosti vzorků proteinů a peptidů. Zjistěte, jak materiály vialek a nespecifická vazba ovlivňují výsledky LC, a získejte tipy pro zlepšení reprodukovatelnosti testů.

Pragolab JARNÍ SLEVY 2026

<h4>Využijte jedinečné příležitosti a vyzkoušejte si vybrané GC / LC kolony a další spotřební materiál ZDARMA!</h4><blockquote><ul><li>promo kód: 2555</li><li>nabídka platná do: 7. 8. 2026</li></ul></blockquote><h5>Na co se akce vztahuje:</h5><ul><li>kompletní portfolio GC kolon – včetně nejnovějších <a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/br_5q_gc_column_campaign_5994_7596en_agilent_baca38e505.pdf">5Q GCMS</a></li><li>většinu HPLC kolon - včetně nejnovějších ultra-inertních kolon <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/br_altura_hplc_columns_ultra_inert_technology_5994_8687en_agilent_517a6c36a7.pdf">Altura</a></li><li>vybraný spotřební materiál pro přípravu vzorků - včetně nejnovějších <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/article/5711">SPE kolonek PFAS WAX</a> s duální fází</li><li>a některé produkty Crosslab a OneNote – včetně <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/flyer_quick_connect_fittings_infinitylab_5991_5164_EN_agilent_0d67eecd72/flyer-quick-connect-fittings-infinitylab-5991-5164EN_agilent.pdf">Quick Connect Agilent InfinityLab Quick Connect Fittings</a> a <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/fl_infinitylab_quickturn_5994_7793en_agilent_819d965d19.pdf">Quick Turn fitinků</a> Agilent InfinityLab Quick Turn Fittings.</li></ul><blockquote><a target="_blank" rel="noopener noreferrer" href="https://www.hpst.cz/sites/default/files/download/2025-12/2555EligibleProductsList_25Sep25-komprimovan%C3%BD.pdf">Kompletní nabídka produktů v akci "Try and Buy"</a></blockquote>Lhůta 50 dnů na vyzkoušení bez platby předem. V případě nespokojenosti vrácení produktu zpět

Altium Akce Try and Buy na GC, HPLC kolony a jiný spotřební materiál

Tradiční postupy purifikace proteinů se v mnoha laboratořích stále opírají o ultracentrifugaci a dlouhé inkubační kroky. Tyto přístupy často zahrnují vícekrokové protokoly, prodloužené doby analýzy a použití specializovaného vybavení. V důsledku toho mohou být pracovní postupy složité, časově náročné a náchylné k variabilitě výsledků.Purifikace proteinů pomocí magnetických kuliček nabízí rychlejší, jednodušší a efektivnější alternativu, protože umožňuje selektivní zachycení a rychlou magnetickou separaci.<h3>Omezení konvenčních metod purifikace proteinů</h3>Ve standardních laboratorních postupech purifikace proteinů obvykle zahrnuje:<ul><li>Více pracovních kroků</li><li>Centrifugaci a ekvilibraci kolony</li><li>Závislost na specializovaném vybavení</li><li>Dlouhé doby zpracování</li></ul>Tyto faktory přinášejí několik výzev:<ul><li>Zvýšená složitost pracovních postupů</li><li>Variabilita mezi jednotlivými cykly, což snižuje reprodukovatelnost</li><li>Riziko ztráty vzorku při manipulaci, zejména u malých objemů vzorků nebo omezených množství cílových látek</li><li>Omezená kapacita kvůli manuální manipulaci</li></ul><h3>Princip purifikace pomocí magnetických kuliček</h3>Magnetické kuličky umožňují selektivní zachycení proteinů díky funkcionalizaci povrchu.Každá kulička je potažena streptavidinem, na který se váže biotinylovaný ligand. Tento ligand lze vybrat na základě cílového analytu a může zahrnovat:<ul><li>Protilátky</li><li>Aptamery</li><li>Fragmenty antigenu</li></ul>Tento krok poskytuje kuličkám specificitu potřebnou pro vazbu cílového analytu.<h3>Materiály a klíčové komponenty</h3><h4>Magnetické kuličky</h4><ul><li>Povrch potažený streptavidinem</li><li>Funkcionalizace biotinylovanými ligandy</li></ul><h4>Ligandy</h4><ul><li>Voleny dle cílového analytu</li><li>Navázané na kuličky před purifikací</li></ul><h3>Postup krok za krokem</h3><h4>1. Aktivace kuliček</h4>Navázání biotinylovaného ligandu na kuličky.<h4>2. Promývání</h4>Odstranění nečistot po funkcionalizaci.<h4>3. Inkubace se vzorkem</h4>Navázání cílového analytu na ligand.<h4>4. Magnetická separace</h4>Umístění vzorku na magnet:<ul><li>Magnetické kuličky (s navázaným analytem) se přitáhnou ke stěně nádoby</li><li>Kapalná fáze se oddělí</li></ul><h4>5. Promývání</h4>Promytí kuliček, zatímco zůstávají imobilizovány magnetickým polem, pro odstranění:<ul><li>Slabých interakcí</li><li>Nespecifických vazeb</li></ul><h4>6. Eluce</h4>Uvolnění analytu pomocí elučního roztoku.<h3>Následné aplikace</h3>Po eluci je purifikovaný vzorek připraven k další analýze, včetně:<ul><li>LC-MS</li><li>Western blot</li><li>ELISA</li><li>Testování aktivity</li></ul><h3>Výhody pracovních postupů založených na magnetických kuličkách</h3>Purifikace založená na magnetických kuličkách nabízí několik klíčových výhod:<h4>Rychlost a jednoduchost</h4><ul><li>Není nutná centrifugace</li><li>Není potřeba složité vybavení</li></ul><h4>Zlepšená reprodukovatelnost</h4><ul><li>Konzistentní magnetická separace</li><li>Snížená variabilita mezi jednotlivými cykly</li></ul><h4>Snížená ztráta vzorku</h4><ul><li>Méně kroků při manipulaci</li><li>Vhodné pro malé objemy vzorků nebo omezená množství cílových látek</li></ul><h4>Škálovatelnost a kapacita</h4><ul><li>Možnost automatizace</li><li>Podporuje formáty mikrodestiček:<ul><li>96-jamkové destičky</li><li>384-jamkové destičky</li></ul></li></ul><h3>Vlastnosti magnetických kuliček Biozen™</h3>Magnetické kuličky Biozen™ od společnosti Phenomenex zahrnují několik konstrukčních vlastností:<ul><li>Hydrofilní kopolymerový povrch<ul><li>Snižuje nespecifické hydrofobní interakce</li></ul></li><li>Biotinylovaný BSA spacer<ul><li>Působí jako spojovací článek ke streptavidinu</li><li>Pomáhá orientovat záchytné ligandy</li></ul></li><li>Monodisperzní ligandy<ul><li>Optimalizovaná vazebná kapacita</li></ul></li></ul>Tyto vlastnosti přispívají k:<ul><li>Snížení nespecifické vazby</li><li>Lepší reprodukovatelnosti</li><li>Čistším eluátům</li></ul><h3>Kdy použít magnetické kuličky</h3>Čištění pomocí magnetických kuliček je zvláště vhodné, když:<ul><li>Objemy vzorků jsou malé</li><li>Je vyžadována rychlá doba zpracování</li><li>Je omezené vybavení</li><li>Jsou potřebné pracovní postupy s vysokou propustností</li></ul><h3>Závěr</h3>Purifikace proteinů pomocí magnetických kuliček zjednodušuje tradiční pracovní postupy tím, že eliminuje odstřeďování a snižuje složitost. Díky selektivnímu zachycování a účinné magnetické separaci umožňuje rychlejší zpracování, lepší reprodukovatelnost a kompatibilitu s automatizovanými systémy s vysokou propustností.

Purifikace proteinů pomocí magnetických kuliček: Rychlejší a efektivnější pracovní postup

Akademie

Video

Článek

Produkt

Je autorizovaným distributorem společnosti Agilent Technologies pro Českou republiku v oblastech chromatografie (GC, HPLC, UHPLC, kapilární elektroforéza) a hmotnostní spektrometrie (GC/MS, LC/MS, CE/MS), disolučních aparátů, molekulární (UV-Vis, UV-Vis-NIR, fluorescence, FTIR analyzátory a mikroskopy) a atomové spektroskopie (ICP-OES, ICP-MS, AAS), a v neposlední řadě také v oblasti molekulární biologie a genomiky (analýza nukleových kyselin a proteinů, microarray skenery a skla, PCR & RT-PCR, qPCR, řešení pro NGS, reagencie pro mutagenezi a klonování, sondy FISH a další).

Altium International

Společnost Phenomenex je celosvětovým technologickým lídrem, který se zabývá vývojem nových řešení v oblasti analytické chemie, jež řeší požadavky analytiků na separace a purifikace v průmyslových, vládních a akademických laboratořích.

Phenomenex

Jsme jedním z lídrů na českém a slovenském trhu v oblasti laboratorních zařízení a svým zákazníkům přinášíme špičkové přístroje z oboru analytické chemie a fyzikálních měření více než 25 let.

Pragolab

Agilent Technologies

Bruker

Shimadzu Corporation

When it comes to LC analysis, a common question for users is, “Which LC column should I choose?” The column is the heart of the LC system, which plays a pivotal role in ensuring high-resolution separation. The fundamental knowledge of column selection can hence help you decide the optimal column for your specific application.
In this second series of Understanding LC Columns webinar, we will introduce the different mode of separation in liquid chromatography including reversed phase, normal phase and HILIC. We will discuss some of the common applications for each of these separation techniques. The difference in column chemistry and column specifications can significantly impact your separation. This webinar will also cover the different column chemistry and the considerations when selecting a column for the different applications.
Learning Objectives
<ul>
<li>Understand the different mode of separation and their application in liquid chromatography.</li>
<li>Understand the different column chemistry and their separation mechanisms.</li>
</ul>
<blockquote>
Presenter: Dr. Derrick Tan (Application Chemist (Consumables), Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Dr. Derrick Tan obtained his PhD in chemistry from Nanyang Technological University, Singapore. He has more than 7 years of research experience in small molecules, nucleic acids, peptides and protein synthesis, separation and purification. He joined Shimadzu (Asia Pacific) Pte Ltd in 2021 as an Application Chemist (Consumables). At Shimadzu, he is responsible for the technical support and application of consumables in chromatography and spectroscopy. Products include LC and GC columns, UV/Vis cuvettes, sample vials and accessories.
</blockquote>

Shimadzu: Understanding LC Columns - Difference in Column Types and Phases

Understanding LC Columns - Difference in Column Types and Phases

We will introduce the different mode of separation in liquid chromatography including reversed phase, normal phase and HILIC.

Understanding LC Columns - Column Types and Phases

The modern laboratory has data collected and reported from a variety of different instruments and locations.
Agilent’s Enterprise Content Manager can store that data and make metadata available for searching through a variety of data specific filters or customized PDF templates. The Metadata ReUse program can then help query and combine that data into an XML/Excel format for further reporting.
Key Learning Objectives
<ul>
<li>Introduction to the ECM-XT Web Client Interface
</li>
<li>MetaData Reuse tool features overview
</li>
<li>XML/Excel Export function of the the MetaData Reuse Tool
</li>
</ul>
<blockquote>
Presenter: Richard Mutkoski (Laboratory Informatics Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Richard Mutkoski began with Agilent (HP) in 1995. He has been an Application Engineer in Agilent’s Laboratory Informatics for the past 18 years. Prior to his career at Agilent Rich spent 12 years in a variety of small pharmaceutical firms in both R&amp;D and QA/QC capacities affording him first-hand knowledge of those workflows, bottlenecks and challenges.
</blockquote>

Agilent Technologies: The Power of Using Existing Meta-Data for Lab Wide Reporting

The Power of Using Existing Meta-Data for Lab Wide Reporting

Introduction to the ECM-XT Web Client Interface.

The promises of the digital lab are enticing but getting there sometimes seems daunting.
This presentation will discuss how to digitize lab operations from start to finish, which is especially relevant in a world where working remotely is becoming increasingly common. In the course of this journey, how can you realize your aspirations and create sustainable outcomes, all while dealing with varied lab environments? The good news is that you can start today. This talk will show how you can prepare the ground for a digital lab, how to improve efficiency of your lab operations, and what it takes to implement an end-to-end solution to manage all content, context, and results in the lab.
<blockquote>
Presenter: Nicolas Louvet (Software Product Manager, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Nicolas Louvet joined Agilent Technologies in 2005 as an ELN Services Consultant in the Software &amp; Informatics Division, became the Product Manager for OpenLab ELN, and currently serves as the Software Product Manager for SLIMS. He has more than 15 years of experience working directly with clients needing to digitalize their laboratory processes. His area of expertise is focused on workflow and content management in the laboratory.
</blockquote>

Agilent Technologies: How to Achieve a Fully Digital, End-to-End Analytical Workflow with CDS, ELN and LIMS

How to Achieve a Fully Digital, End-to-End Analytical Workflow with CDS, ELN and LIMS

How to digitize lab operations from start to finish, which is especially relevant in a world where working remotely is becoming increasingly common.

How to Achieve a Fully Digital, End-to-End Workflow

In this seminar we will build a qualitative method for peak purity.
We will look at an LC/MS walk up method work flow within the OpenLab CDS environment. We will look at LC/MS purity functions, the summing of SIM ions and spectral tips and tricks. 
<blockquote>
Presenter: Sue D&#39;Antonio (Applications Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Sue D’Antonio joined Agilent (HP at that time) more than 30 years ago. For the last 15 years Sue has been an Applications Scientist in the Liquid Phase Separations Group. Sue’s application support extends to HPLC, UHPLC, CE and LCMS across a wide range of markets and customers.
</blockquote>

Agilent Technologies: Tips and Tricks for LC/MS Purity workflow in OpenLab CDS softwar

Tips and Tricks for LC/MS Purity workflow in OpenLab CDS softwar

LC/MS walk up method work flow within the OpenLab CDS environment.

Tips and Tricks for LC/MS Purity workflow in OpenLab CDS

This webinar will give an overview of Agilent’s Walkup add-on software which can increase the efficiency of LC and LC/MS systems with multiple users.
The Walkup software allows for an administrator to oversee all aspects of the chromatography system and defines specified user access. A general overview of the Walkup software platform will be given with examples utilized to show the convenience for a multiple user chromatography system.

Agilent Technologies: Inc the Spd, Effic, Robustness, and Ease of Operation of Your Multi-use LC and LC/MS Sys by Incorporating Agilent

Inc the Spd, Effic, Robustness, and Ease of Operation of Your Multi-use LC and LC/MS Sys by Incorporating Agilent

Overview of Agilent’s Walkup add-on software which can increase the efficiency of LC and LC/MS systems with multiple users.

Inc the Spd, Effic, Robustness, and Ease of Operation

HPLC Autosampler injector programming has been a feature of Agilent autosamplers since the early days of autosampler innovations.
The injector program contains a series of simple commands that maybe performed during an injection cycle which enables a sample pretreatment. During this session we will discuss how these commands can be employed to make dilutions to reduce sample preparation time. The versatility of the program allows multi-level dilutions with minimum carryover.
<blockquote>
Presenter: Greg Hunlen (Application Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Gregory Hunlen has served as an Application Scientist with Agilent Technologies since 2012. He applies his expertise to the Agilent Infinity II series of LC instruments, providing knowledge of LC solutions such as two dimensional LC and LCMSD workflows. He also delivers product demonstrations and lunch &amp; learns. As a key value, Greg likes to talk about maximizing the use of all features our technologies provide. Often, instruments are under-utilized perhaps as a lack of understanding of all that they can offer. When we explore these features, we discover that we can truly optimize the efficiencies of our instruments. Prior to joining Agilent, Greg spent 20 + years in the pharmaceutical research industry on the liquid phase chromatography side of drug development.
</blockquote>

Agilent Technologies: Strategies for HPLC Analysis using Autosampler Injector Programming

Strategies for HPLC Analysis using Autosampler Injector Programming

How these commands can be employed to make dilutions to reduce sample preparation time.

Strategies for HPLC Analysis using Autosampler Injector

Find out about the next generation of software from Agilent.
OpenLab CDS can streamline your lab operations with a single, secure data system that combines chromatography and mass spectrometry into one software platform. OpenLab CDS can control your Agilent GC, LC, single quadrupole GC/MS, LC/MS instruments as well as other vendors’ instruments in the lab, and save substantial time managing software and training staff.
<blockquote>
Presenter: Shawn Anderson (Director of Marketing, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Shawn Anderson is the Associate Vice President of Marketing for the Software and Informatics Division at Agilent Technologies. He has over 28 years of experience in the chromatography and purification/separations market space. His career has developed from bench work in biotech to field support, applications, sales, product management, and product marketing. Shawn is proud to have “learned and led” at some of the most well-respected vendors in the industry such as GE Healthcare, Dionex, Bio-Rad, and now Agilent, designing, launching, and marketing next generation hardware, software and consumable products. He is passionate about end-user and customer voice and believes that the lost sale is often the best learning opportunity. Finally, he is an optimistic futurist, and looks forward to the continuing democratization of science and technology.
</blockquote>

Agilent Technologies: OpenLab CDS-The future of Agilent Chromatography Data System for HPLC, GC, GCMS and LCMS

OpenLab CDS-The future of Agilent Chromatography Data System for HPLC, GC, GCMS and LCMS

Find out about the next generation of software from Agilent.

OpenLab CDS The future of Agilent Chromatography Data System

Two Dimensional HPLC (2D-LC) is a technique where two orthogonal techniques are brought together in one system for the purposes of increasing peak capacity as well as providing additional information from complex mixtures and analytes.
In this presentation we will show the analysis of a mAB with a HIC first dimension followed by a RP/desalting column in the second dimension. The second dimension column will removes salts from the mobile phase which will then allow the eluent to be analyzed by an mass spectrometer without the salts that could cause issues with the MS source.
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Presenter: Bob Giuffre (Application Engineer, Agilent Technologies, Inc.)
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Bob Giuffre has been working with HP/Agilent for almost thirty years as an Application Scientist for the HPLC product line. During that time Bob has worked on applications ranging from small molecule pharmaceutics, to large molecule polymers, to monoclonal antibodies. Currently, Bob is working in the area of ultra-high pressure two-dimensional liquid chromatography as a means to increase peak capacity for complex formulations. Bob has authored a chapter on the use of Diode-Array Detection in Pharmaceuticals. He is currently writing a chapter on Troubleshooting Peptide and Proteins Analysis using LC-MS.
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 Presenter: Sandeep Kondaveeti (Application Scientist, Agilent Technologies, Inc.)
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Sandeep is a Philadelphia native and currently works as an applications chemist responsible for developing Bio-pharma workflow applications with Agilent Technologies supporting bio-columns portfolio. Prior to joining Agilent, Sandeep undertook graduate studies at Temple University in Bioinorganic chemistry focused in developing Inorganic metal clusters based on Photosynthetic metalloenzymes and worked as Process Analytical Scientist for Janssen Pharmaceuticals through Eurofins.
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Agilent Technologies: 2D HPLC of Monoclonal Antibody with Novel Hydrophobic Interaction coupled to Reverse Phase Desalting with MS de

2D HPLC of Monoclonal Antibody with Novel Hydrophobic Interaction coupled to Reverse Phase Desalting with MS de

Analysis of a mAB with a HIC first dimension followed by a RP/desalting column in the second dimension.

2D HPLC of Monoclonal Antibody with Novel Hydrophobic

Organoids and spheroids are attractive 3D cell culture models because they mimic specific in vivo physiological conditions, can be developed from human cells, and contribute towards animal-free research. Their analysis brings light on cell-cell and cell-microenvironment molecular composition and interactions, which creates opportunities in cancer research and drug screening. However, 3D molecular characterization is required for a complete understanding of the molecular composition and chemical changes of the entire system.Our workflow enables studying cell-cell interactions, invasive cell migration, tumor progression, and other spatially relevant interactions within 3D cell culture models which could be monitored in 3D using MALDI MSI. We analyzed a colon cancer-fibroblast co-culture spheroid model developed by Prof. Rüdiger Rudolf and team, and were able to distinguish between cell type specific small molecules in an unbiased manner and high throughput. Therefore, our 3D technology could enable the identification of desirable markers for studying biological mechanisms typical to each cell type, which could lay the grounds for developing 3D-MSI based drug screenings.<blockquote>Presenter: Stefania-Alexandra Iakab, Ph.D. (Team Leader Spatialomics, Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Mannheim, Germany)Stefania-Alexandra Iakab received her Ph.D. from the Rovira i Virgili University in Tarragona, Spain. She developed gold and silicon-based nanostructured surfaces for multimodal imaging combining matrix-free mass spectrometry imaging and surface assisted Raman spectroscopy. She is currently leading the Spatialomics team at CeMOS, Germany. Her research focuses on developing MSI workflows for pharma applications on 3D cell culture models.Presenter:&nbsp;Janina Oetjen, Ph.D. (Application Specialist MALDI Imaging, Bruker Daltonics, Bremen, Germany)Janina Oetjen did her Ph.D. in molecular microbiology at the University of Bremen in 2009. As employee at the MALDI Imaging Lab of the University of Bremen, she provided analytical service in the field of mass spectrometry imaging. At SCiLS, she headed the SCiLS™ Analytical Services division and acted as a consultant for the software development. Since summer 2018, she joined Bruker as Application Scientist for MALDI Imaging and is responsible for timsTOF fleX and timsTOF fleX MALDI-2 demos.</blockquote>

Bruker: Monitoring small molecules from a colon cancer spheroid model in 3D using MALDI MSI

Monitoring small molecules from a colon cancer spheroid model in 3D using MALDI MSI

Learn about 3D cell culture models which could be monitored in 3D using MALDI MSI.

Monitoring small molecules using MALDI MSI

The polymer industry is one of the world’s largest industrial sectors. &nbsp;Almost all consumer products are composed of polymers of some sort. &nbsp;These products range across items like automobiles, pharmaceuticals, toys, packaging, computer and cell phones, and so on. &nbsp;There are a few areas where characterizing these polymer-based objects can be important: first is performing a quick polymer identification for recycling purposes; second is quality control to identify product defects or failures; and third is reverse engineering to facilitate product development in a highly competitive marketplace. &nbsp;Vibrational spectroscopy (infrared (IR) and Raman) is especially useful for the analysis of polymers in an industrial setting. &nbsp;IR and Raman are highly specific and sensitive tools that are non-destructive and easy to use. &nbsp;Examples will be presented demonstrating the effectiveness of IR and Raman analysis in each of these categories.Mass Spectrometry (MS) has become an indispensable tool for polymer analysis and has been widely used to study polymer structure and composition, end-groups and additives, molecular weight distribution, and so on. &nbsp;MS analysis is extremally sensitive, allowing the detection and identification of minor polymer components and synthesis by-products, as well as low-level impurities and products of decomposition. &nbsp;Matrix Assisted Laser Desorption Ionization (MALDI) allows to perform rapid MS analysis without lengthy pre-separation and with minimal sample preparation. &nbsp;MALDI MS can provide structural and copolymer composition information, as well as be used for imaging of polymer surfaces, characterization of complex polymer mixtures and many other analytical tasks. &nbsp;In this webinar, we will discuss several MS analytical techniques and their applications for polymer analysis using real life examples from the industry and academia.<blockquote>Presenter: Dr. Tom Tague (Applications Manager and Chair of the Safety Committee Bruker Corporation)Dr. Tom Tague is the Applications Manager (FTIR and Raman products) and Chair of the Safety Committee for Bruker Corporation. He is also a member of the Visiting Advisory Committee of the Metropolitan Museum of Art in New York and the Advisory Board of Amplified Sciences. Dr. Tague received his Ph.D. from the University of Utah in Physical Chemistry and his B.S. in Chemistry, from the University of Texas at San Antonio. He conducted his postdoctoral research at the University of Virginia working with professor Lester Andrews. He is a member of the American Chemical Society, the Society for Applied Spectroscopy, the American Physical Society, and the Optical Society of America. Dr. Tague is active in developing new methods and instrumentation with the goal of improving the sensitivity and detection limits of spectroscopy related applications. Dr. Tague has more than 80 publications and 5 patents.Presenter:&nbsp;Dr. Artem FilipenkoDr. Artem Filipenko has over 15 years of industry experience in development of innovative methods and instrumentation for various analytical applications, including food, environmental, industrial, and homeland security. His area of professional expertise ranges from Ion Mobility, Mass Spectrometry, and Gamma Spectroscopy to molecular and nuclear physics. Artem holds a doctorate degree in molecular physics and master’s degree in microelectronics from the National Nuclear Research University in Moscow, as well as master’s degree in business administration from Babson College in Boston.</blockquote>

Bruker: Effectively Analyzing Polymers Using IR, Raman Spectroscopy and MALDI-TOF Mass Spectrometry

Effectively Analyzing Polymers Using IR, Raman Spectroscopy and MALDI-TOF Mass Spectrometry

In this webinar, we will discuss several MS analytical techniques, IR and Raman and their applications for polymer analysis 