<p>Vážení zákazníci,</p><p>přichází <strong>JARNÍ AKCE 2026</strong>, během které můžete využít <strong>zvýhodněné ceny na široký sortiment spotřebního materiálu</strong> pro vaši laboratoř.</p><h4><strong>Nabízíme vše, co potřebujete pro každodenní provoz i náročné analýzy:</strong></h4><ul><li>kolony pro <strong>HPLC, UHPLC, BioLC</strong></li><li>kolony pro <strong>GC</strong> a <strong>IC</strong></li><li>spotřební materiál pro přípravu vzorků</li><li>drobné laboratorní vybavení</li></ul><p>Kvalitní spotřební materiál je základem spolehlivých výsledků. Využijte proto tuto příležitost a doplňte své zásoby za výhodnějších podmínek.</p><blockquote><p>Akce je platná na území ČR <strong>od 1. 4. do 31. 5. 2026</strong>.</p><p>Kontaktujte nás jednoduše na e-mailu: <strong>chromatografie@pragolab.cz</strong>.</p></blockquote><h4><strong>−30 % HPLC kolony</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products/423"><strong>Hypersil GOLD</strong></a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products?category=48&amp;manufacturerItems=3&amp;search=accucore"><strong>Accucore</strong></a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=Syncronis"><strong>Syncronis</strong></a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=Hypercarb"><strong>Hypercarb</strong></a></li></ul><h4><strong>−20 % BioLC kolony a kolony pro speciální aplikace</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=MabPac"><strong>MabPac</strong></a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=ProPac"><strong>ProPac</strong></a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=PepMap"><strong>PepMap</strong></a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=EasySpray"><strong>EasySpray</strong></a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=Acclaim"><strong>Acclaim</strong></a></li></ul><h4><strong>−20 % GC kolony</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/products?category=7&amp;distributorItems=42&amp;search=TraceGOLD"><strong>TraceGOLD</strong></a> — <a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/products?category=7&amp;distributorItems=42&amp;search=Trace"><strong>Trace GC</strong></a> — <a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/products?category=7&amp;distributorItems=42&amp;search=TracePLOT"><strong>Trace PLOT</strong></a></li></ul><h4><strong>−25 % IC kolony a spotřební materiál</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?instrumentationItems=28-34&amp;mainauthorItems=3&amp;search=columns"><strong>Kolony</strong></a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?instrumentationItems=28&amp;mainauthorItems=3&amp;search=EGC"><strong>EGC</strong></a> — supresory — vialky</li></ul><h4><strong>−25 % </strong><a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/Pragolab_vialka_f386946736/Pragolab_vialka_.pdf"><strong>Vialky Pragolab</strong></a></h4><ul><li>Vialky — septa — kity</li></ul><h3><strong>Přehled nejnovější literatury z oblasti spotřebního matriálu od Thermo Scientific v knihovně </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?from=2025&amp;instrumentationItems=20&amp;manufacturerItems=2&amp;to=2026"><strong>LabRulezLCMS</strong></a></h3><ul><li><strong>Instrument and LC column migration for the purification and analysis of synthetic oligonucleotides</strong> (Aplikace | 2025)</li><li><strong>Evolution of Ion-Exchange columns for biologics characterization</strong> (Prezentace | 2025)</li><li><strong>Advancing Low flow LC/MS for single cell proteomics with variable flow and 50 cm microfabricated pillar array columns</strong> (Prezentace | 2025)</li><li><strong>Wide-ranging polynucleotide separation capabilities using Reversed Phase particles with variable pore geometry</strong> (Postery | 2025)</li><li><strong>Evaluation of batch-to-batch consistency of reversed phase HPLC columns for long-term method validation</strong> (Postery | 2025)</li><li><strong>Efficient tandem capillary flow LC-MS with short μPAC columns and a single ionization source</strong> (Postery | 2025)</li><li><strong>At the intersection between chromatographic performance, ESI efficiency and instrument productivity: nano to capillary flow LC/MS on long μPAC Columns</strong> (Postery | 2025)</li><li><strong>Achieving consistent SEC performance through the use of 3 μm, 550 Å monodisperse media in novel bioinert column hardware</strong> (Postery | 2025)</li><li><strong>Improved accuracy and identification in label-free quantitative proteomics with the OptiSpray Ion Source and separation cartridge with integrated replaceable emitters</strong> (Postery | 2025)</li><li><strong>Accucore columns Resolve all your impurities without feeling the pressure</strong> (Ostatní | 2025)</li><li><strong>SureSTART Vial Quick Select Guide </strong>(Ostatní | 2025)</li><li><strong>Sensitive cationic lipids impurities analysis with quantitation by charged aerosol detection and simultaneous mass confirmation by MS</strong> (Aplikace | 2025)</li><li><strong>Thermo Scientific Viper TQ UHPLC Fittings</strong> (Brožury a specifikace | 2025)</li><li><strong>HPLC vials and well plates - Every drop counts </strong>(Brožury a specifikace | 2025)</li></ul><h3><strong>Vybrané nejnovější </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinars?companyId=42"><strong>webináře</strong></a><strong> Thermo Scientific z oblasti spotřebního materiálu</strong></h3><h5><strong>1. Mastering Single Cell Proteomics: Daily Excellence with μPAC Neo Plus Trap-and-Elute Workflow</strong></h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/5789"><strong>ZÁZNAM</strong></a> | Proběhlo Út, 27.1.2026</li></ul><p>Zjistěte, jak dosáhnout robustní, vysoce citlivé proteomiky jednotlivých buněk pomocí systému μPAC Neo Plus trap-and-elute LC-MS se systémy Vanquish Neo a Orbitrap.</p><h5><strong>2. Monodisperse inert HPLC columns for large molecule analysis in biopharma</strong></h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/5729"><strong>ZÁZNAM</strong></a> | Proběhlo Út, 9.12.2025</li></ul><p>Připojte se k nám na zajímavém webináři o pokročilých chromatografických metodách pro analýzu velkých biomolekul.</p><h5><strong>3. Reduce analyte loss in LC workflows: The role of vial and surface chemistry</strong></h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/5675"><strong>ZÁZNAM</strong></a> | Proběhlo Po, 17.11.2025</li></ul><p>Objevte strategie pro zvýšení výtěžnosti vzorků proteinů a peptidů. Zjistěte, jak materiály vialek a nespecifická vazba ovlivňují výsledky LC, a získejte tipy pro zlepšení reprodukovatelnosti testů.</p>

Pragolab JARNÍ SLEVY 2026

<h4><strong>Využijte jedinečné příležitosti a vyzkoušejte si vybrané GC / LC kolony a další spotřební materiál ZDARMA!</strong></h4><blockquote><ul><li><strong>promo kód: </strong>2555</li><li><strong>nabídka platná do: </strong>7. 8. 2026</li></ul></blockquote><h5><strong>Na co se akce vztahuje:</strong></h5><ul><li><strong>kompletní portfolio GC kolon</strong> – včetně nejnovějších <a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/br_5q_gc_column_campaign_5994_7596en_agilent_baca38e505.pdf"><strong>5Q GCMS</strong></a></li><li><strong>většinu HPLC kolon</strong> - včetně nejnovějších ultra-inertních kolon <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/br_altura_hplc_columns_ultra_inert_technology_5994_8687en_agilent_517a6c36a7.pdf"><strong>Altura</strong></a></li><li><strong>vybraný spotřební materiál pro přípravu vzorků</strong> - včetně nejnovějších <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/article/5711"><strong>SPE kolonek PFAS WAX</strong></a> s duální fází</li><li>a <strong>některé produkty Crosslab a OneNote</strong> – včetně <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/flyer_quick_connect_fittings_infinitylab_5991_5164_EN_agilent_0d67eecd72/flyer-quick-connect-fittings-infinitylab-5991-5164EN_agilent.pdf"><strong>Quick Connect Agilent InfinityLab Quick Connect Fittings</strong></a> a <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/fl_infinitylab_quickturn_5994_7793en_agilent_819d965d19.pdf"><strong>Quick Turn fitinků</strong></a> Agilent InfinityLab Quick Turn Fittings.</li></ul><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://www.hpst.cz/sites/default/files/download/2025-12/2555EligibleProductsList_25Sep25-komprimovan%C3%BD.pdf"><strong>Kompletní nabídka produktů v akci "Try and Buy"</strong></a></p></blockquote><p><strong>Lhůta 50 dnů </strong>na vyzkoušení bez platby předem. V případě nespokojenosti vrácení produktu zpět</p>

Altium Akce Try and Buy na GC, HPLC kolony a jiný spotřební materiál

<p><strong>Tradiční postupy purifikace proteinů se v mnoha laboratořích stále opírají o ultracentrifugaci a dlouhé inkubační kroky. Tyto přístupy často zahrnují vícekrokové protokoly, prodloužené doby analýzy a použití specializovaného vybavení. V důsledku toho mohou být pracovní postupy složité, časově náročné a náchylné k variabilitě výsledků.</strong></p><p>Purifikace proteinů pomocí magnetických kuliček nabízí rychlejší, jednodušší a efektivnější alternativu, protože umožňuje selektivní zachycení a rychlou magnetickou separaci.</p><h3><strong>Omezení konvenčních metod purifikace proteinů</strong></h3><p><strong>Ve standardních laboratorních postupech purifikace proteinů obvykle zahrnuje:</strong></p><ul><li>Více pracovních kroků</li><li>Centrifugaci a ekvilibraci kolony</li><li>Závislost na specializovaném vybavení</li><li>Dlouhé doby zpracování</li></ul><p><strong>Tyto faktory přinášejí několik výzev:</strong></p><ul><li>Zvýšená složitost pracovních postupů</li><li>Variabilita mezi jednotlivými cykly, což snižuje reprodukovatelnost</li><li>Riziko ztráty vzorku při manipulaci, zejména u malých objemů vzorků nebo omezených množství cílových látek</li><li>Omezená kapacita kvůli manuální manipulaci</li></ul><h3><strong>Princip purifikace pomocí magnetických kuliček</strong></h3><p>Magnetické kuličky umožňují selektivní zachycení proteinů díky funkcionalizaci povrchu.</p><p>Každá kulička je potažena <strong>streptavidinem</strong>, na který se váže <strong>biotinylovaný ligand</strong>. Tento ligand lze vybrat na základě cílového analytu a může zahrnovat:</p><ul><li>Protilátky</li><li>Aptamery</li><li>Fragmenty antigenu</li></ul><p>Tento krok poskytuje kuličkám specificitu potřebnou pro vazbu cílového analytu.</p><h3><strong>Materiály a klíčové komponenty</strong></h3><h4><strong>Magnetické kuličky</strong></h4><ul><li>Povrch potažený streptavidinem</li><li>Funkcionalizace biotinylovanými ligandy</li></ul><h4><strong>Ligandy</strong></h4><ul><li>Voleny dle cílového analytu</li><li>Navázané na kuličky před purifikací</li></ul><h3><strong>Postup krok za krokem</strong></h3><h4><strong>1. Aktivace kuliček</strong></h4><p>Navázání biotinylovaného ligandu na kuličky.</p><h4><strong>2. Promývání</strong></h4><p>Odstranění nečistot po funkcionalizaci.</p><h4><strong>3. Inkubace se vzorkem</strong></h4><p>Navázání cílového analytu na ligand.</p><h4><strong>4. Magnetická separace</strong></h4><p>Umístění vzorku na magnet:</p><ul><li>Magnetické kuličky (s navázaným analytem) se přitáhnou ke stěně nádoby</li><li>Kapalná fáze se oddělí</li></ul><h4><strong>5. Promývání</strong></h4><p>Promytí kuliček, zatímco zůstávají imobilizovány magnetickým polem, pro odstranění:</p><ul><li>Slabých interakcí</li><li>Nespecifických vazeb</li></ul><h4><strong>6. Eluce</strong></h4><p>Uvolnění analytu pomocí elučního roztoku.</p><h3><strong>Následné aplikace</strong></h3><p>Po eluci je purifikovaný vzorek připraven k další analýze, včetně:</p><ul><li>LC-MS</li><li>Western blot</li><li>ELISA</li><li>Testování aktivity</li></ul><h3><strong>Výhody pracovních postupů založených na magnetických kuličkách</strong></h3><p>Purifikace založená na magnetických kuličkách nabízí několik klíčových výhod:</p><h4><strong>Rychlost a jednoduchost</strong></h4><ul><li>Není nutná centrifugace</li><li>Není potřeba složité vybavení</li></ul><h4><strong>Zlepšená reprodukovatelnost</strong></h4><ul><li>Konzistentní magnetická separace</li><li>Snížená variabilita mezi jednotlivými cykly</li></ul><h4><strong>Snížená ztráta vzorku</strong></h4><ul><li>Méně kroků při manipulaci</li><li>Vhodné pro malé objemy vzorků nebo omezená množství cílových látek</li></ul><h4><strong>Škálovatelnost a kapacita</strong></h4><ul><li>Možnost automatizace</li><li>Podporuje formáty mikrodestiček:<ul><li>96-jamkové destičky</li><li>384-jamkové destičky</li></ul></li></ul><h3><strong>Vlastnosti magnetických kuliček Biozen™</strong></h3><p>Magnetické kuličky <strong>Biozen™</strong> od společnosti Phenomenex zahrnují několik konstrukčních vlastností:</p><ul><li><strong>Hydrofilní kopolymerový povrch</strong><ul><li>Snižuje nespecifické hydrofobní interakce</li></ul></li><li><strong>Biotinylovaný BSA spacer</strong><ul><li>Působí jako spojovací článek ke streptavidinu</li><li>Pomáhá orientovat záchytné ligandy</li></ul></li><li><strong>Monodisperzní ligandy</strong><ul><li>Optimalizovaná vazebná kapacita</li></ul></li></ul><p><strong>Tyto vlastnosti přispívají k:</strong></p><ul><li>Snížení nespecifické vazby</li><li>Lepší reprodukovatelnosti</li><li>Čistším eluátům</li></ul><h3><strong>Kdy použít magnetické kuličky</strong></h3><p><strong>Čištění pomocí magnetických kuliček je zvláště vhodné, když:</strong></p><ul><li>Objemy vzorků jsou malé</li><li>Je vyžadována rychlá doba zpracování</li><li>Je omezené vybavení</li><li>Jsou potřebné pracovní postupy s vysokou propustností</li></ul><h3><strong>Závěr</strong></h3><p>Purifikace proteinů pomocí magnetických kuliček zjednodušuje tradiční pracovní postupy tím, že eliminuje odstřeďování a snižuje složitost. Díky selektivnímu zachycování a účinné magnetické separaci umožňuje rychlejší zpracování, lepší reprodukovatelnost a kompatibilitu s automatizovanými systémy s vysokou propustností.</p>

Purifikace proteinů pomocí magnetických kuliček: Rychlejší a efektivnější pracovní postup

Akademie

Video

Článek

Produkt

Je autorizovaným distributorem společnosti Agilent Technologies pro Českou republiku v oblastech chromatografie (GC, HPLC, UHPLC, kapilární elektroforéza) a hmotnostní spektrometrie (GC/MS, LC/MS, CE/MS), disolučních aparátů, molekulární (UV-Vis, UV-Vis-NIR, fluorescence, FTIR analyzátory a mikroskopy) a atomové spektroskopie (ICP-OES, ICP-MS, AAS), a v neposlední řadě také v oblasti molekulární biologie a genomiky (analýza nukleových kyselin a proteinů, microarray skenery a skla, PCR & RT-PCR, qPCR, řešení pro NGS, reagencie pro mutagenezi a klonování, sondy FISH a další).

Altium International

Společnost Phenomenex je celosvětovým technologickým lídrem, který se zabývá vývojem nových řešení v oblasti analytické chemie, jež řeší požadavky analytiků na separace a purifikace v průmyslových, vládních a akademických laboratořích.

Phenomenex

Jsme jedním z lídrů na českém a slovenském trhu v oblasti laboratorních zařízení a svým zákazníkům přinášíme špičkové přístroje z oboru analytické chemie a fyzikálních měření více než 25 let.

Pragolab

Agilent Technologies

C&EN

Evosep Biosystems

Waters Corporation

<p><strong>It takes time, money, and resources to generate laboratory data, so any loss of data is a direct financial loss to your regulated company, as well as a major compliance risk.  Laboratory data must be secured and available to support product quality decisions, both at the time of decision making and for the full duration of the mandated retention period.</strong></p>
<p>A regulated company must ensure its data is retained through the retention period and maintains its integrity during that time.  While external assistance can be sought with activities such as backup and restore, disaster recovery and business continuity planning, archiving, system administration and cybersecurity, the overall responsibility for the regulated data remains with the regulated company.</p>
<p>Key points of discussion will be:</p>
<ol>
<li><strong>Working with Servers</strong></li>
<li><strong>Having a Backup and Restore Plan</strong></li>
<li><strong>Developing a Disaster Recovery Plan</strong></li>
<li><strong>Have a Business Continuity Plan</strong></li>
<li><strong>Archiving Data</strong></li>
<li><strong>Cybersecurity Considerations</strong></li>
<li><strong>Updates to Software</strong></li>
</ol>
<p>Please join us for a live discussion as we explore this topic.  As always, don’t forget to bring your Empower questions for this exclusive opportunity to ask our experts anything about Empower Software.</p>
<blockquote>
<p><strong>Presenter:</strong> Neil Lander (Principal Product Manager, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager focusing on Informatics solutions. He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Fiona O&#39;Leary (Informatics Consultant, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Fiona is an experienced Empower Software and Data Integrity professional working within the EU Professional Services Team.</p>
</blockquote>
<blockquote>
<p>She has ~18 years of experience with Waters, beginning with Technical Support &amp; Empower Training, moving to Empower Consultancy and Workflow Optimization in more recent years. Her focus now is on building Data Integrity into the Lab workflow, Custom Field automation and Electronic Data Review / Reporting optimization.</p>
</blockquote>
<blockquote>
<p>Prior to joining Waters, Fiona spent some years in the GxP Pharmaceutical Manufacturing world, setting the scene for her current goal of helping organizations achieve their scientific and operational objectives efficiently and within compliance guidelines. She graduated from Dublin City University in 2001 with a BSc Hons. Analytical Science.</p>
</blockquote>


Waters Corporation: A Guide to Ensuring Your Data Is Secure and Available from Creation Thru the Full Mandated Retention Period

A Guide to Ensuring Your Data Is Secure and Available from Creation Thru the Full Mandated Retention Period

As always, don’t forget to bring your Empower questions for this exclusive opportunity to ask our experts anything about Empower Software.

Ensuring Your Data Is Secure and Available

<p><strong>Biological samples represent formidable challenges in sample preparation that hold important consequences for LC analysis. Cleaner samples translate to more robust, reproducible LC analysis, with fewer repeat runs, and less equipment down time. The challenge is knowing which sample preparation technique to combine with both your sample and LC method.</strong></p>
<p>In this discussion we will look at some common bio sample preparation techniques, from solvent exchanges to solid phase extractions. We will then discuss how to match the sample preparation technique to both your sample and the desired outcome of your chromatography.</p>
<blockquote>
<p><strong>Presenter:</strong> Melissa Goodlad (Application Engineer, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Melissa Goodlad is an Application Engineer providing applications assistance and technical support for Agilent’s HPLC columns and consumables. Before joining Agilent, Melissa was completing her PhD in Physical Chemistry at UC Merced studying nanoparticle self-assemblies.</p>
</blockquote>


Agilent Technologies: Sample Preparation for Improved LC Bio Separations

Sample Preparation for Improved LC Bio Separations

In this discussion we will look at some common bio sample preparation techniques, from solvent exchanges to solid phase extractions.

<p><strong>Oligonucleotides (oligos) have become fast-growing modalities in recent years. Along with the development of these candidates has come the increased need for robust analytical methods and easy-to-use data analysis workflows to characterize them. Critical quality attributes of oligos include their mass, purity, and sequence – each of which can be difficult, time consuming, and tedious. As such, software that supports these efforts can be of great value.</strong></p>
<p>In this work, we present novel, automated, and integrated software to support these workflows using LC HRAM MS instruments. Purity and sequencing data are presented for a multitude of oligos including antisense, aptamer, and long synthetic samples.</p>
<p>Key Learning Objectives:</p>
<ul>
<li>Techniques for analyzing the critical quality attributes (CQAs) of oligos — including their mass, purity, and sequence</li>
<li>How novel, automated, and integrated software can facilitate the analysis of oligo CQAs using LC–HRAM MS instruments.</li>
<li>Purity and sequencing data for a multitude of oligos including antisense, aptamer, and long synthetic samples</li>
</ul>
<p>Who Should Attend:</p>
<ul>
<li>Researchers analyzing the critical quality attributes (CQAs) of oligos — including their mass, purity, and sequence</li>
<li>Chromatographers</li>
<li>Researchers interested in using LC–HRAM MS instruments for determination of oligo mass, purity and sequence</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Peter Rye (Application Engineer Pre Sales, Agilent Technologies)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Melissa O&#39;Meara (Forensic Science Consultant, C&amp;EN Media Group)</p>
</blockquote>


C&EN: Recent Advances in Oligo Purity and Sequence Determination by LCMS

Recent Advances in Oligo Purity and Sequence Determination by LCMS

In this work, we present novel, automated, and integrated software to support oligos workflows using LC HRAM MS instruments.

Advances in Oligo Purity and Sequence Determination by LCMS

<p><strong>In UV-VIS-NIR spectroscopy, there is a wide range of instruments available that cover all measurements from routine to research.</strong></p>
<p>We will discuss in this presentation some of the key performance differentiators between spectrometers as well as the impact that these have on measurement capabilities.</p>
<blockquote>
<p><strong>Presenter:</strong> Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.</p>
</blockquote>


Agilent Technologies: Picking the Right UV-Vis-NIR For Me: What Specs Do I Need?

Picking the Right UV-Vis-NIR For Me: What Specs Do I Need?

Key performance differentiators between UV-VIS-NIR spectrometers as well as the impact that these have on measurement capabilities.

<p><strong>How do I collect a UV-VIS Baseline?  Why is my concentration curve non-linear?  What amount of averaging do I need to do?</strong></p>
<p>Join us in this webinar where we will discuss some of the most common questions for UV-VIS data collection.</p>
<blockquote>
<p><strong>Presenter:</strong> Scott Melis, PhD (Application Scientist, gilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.</p>
</blockquote>


Agilent Technologies: Common UV-Vis Questions Answered

Common UV-Vis Questions Answered

Join us in this webinar where we will discuss some of the most common questions for UV-VIS data collection.

<p><strong>If you are new to our technology or just getting started this event is for you. We will introduce the basics of the Evosep One technology and include hot-from-the-lab data from our users. Nicolai Bache, Head of Applications at Evosep, will host the event, bringing you up to speed with the latest additions to the Evosep One technology.</strong></p>
<p>The Evosep One is a plug-and-play standardized platform designed for robustness and high throughput. It has been designed specifically to address and eliminate the prevalent challenges associated with throughput and robustness of nano-flow LC-MS workflows while maintaining sufficient sensitivity and resolving power for clinical applications.</p>
<p>The Evosep One technology is centered around the Evotip and integrates sample preparation with LC-MS. The Evotip is essentially a disposable trap column in a pipette tip format with a small plug of C18 stationary phase at the bottom of the tip. The Evotips are used to desalt and clean up the samples prior to LC-MS analysis, however, the traditional subsequent steps of eluting, drying down, re-suspending the samples from tips are completely omitted and instead the tips are loaded directly into Evosep One for analysis.</p>
<p>The system uses four low-pressure syringe pumps in parallel to elute samples from the Evotip with a chromatographic gradient. The resulting gradient with the pre-separated sample is moved into a storage loop, that is then switched in-line with a single high-pressure pump and an analytical separation column for focusing. With this simplified workflow on pre-set gradients, the Evosep One offers a throughput from 30 to 300 samples per day.</p>
<h5><strong>1. A beginner’s guide to the Evosep One</strong></h5>
<ul>
<li>Presenter: Nicolai Bache (Head of applications at Evosep)</li>
</ul>
<p>The Evosep One technology is centered around the Evotip and integrates sample preparation with LC-MS. The Evotip is essentially a disposable trap column in a pipette tip format with a small plug of C18 stationary phase at the bottom of the tip. The Evotips are used to desalt and clean up the samples prior to LC-MS analysis, however, the traditional subsequent steps of eluting, drying down, re-suspending the samples from tips are completely omitted and instead the tips are loaded directly into Evosep One for analysis.</p>
<h5><strong>2. To be announced</strong></h5>
<ul>
<li>Presenter: Anh Reynolds (Associate Scientist at BMS)</li>
</ul>
<h5><strong>3. Clinical proteomics in the fast lane – Combination of robust LC, timsTOF based IMS-MS and GPU powered data processing</strong></h5>
<ul>
<li>Presenter: Allan Stensballe (Industrial PHD at Aalborg University)</li>
</ul>
<p>Mass spectrometrists who have been in the proteomics field long enough to be users of both early gel-free and gel-based strategies have learned to appreciate robust and fast analysis pipelines. As clinical proteomics projects now increasingly moves towards larger cohort studies and integration in multiOmics projects the stability and reproducibility of LC-MS to data analysis is imperative.  In this talk I will highlight our experiences, project examples and thoughts on use of clinically optimized and standardized workflows.</p>


A beginner’s guide to Evosep

The Evosep One is a plug-and-play standardized platform designed for robustness and high throughput.

<p><strong>At the analytical scale, liquid chromatography (LC) is widely used by scientists to separate, quantify, and characterize components in mixtures. At the preparative scale, LC is primarily used to purify samples for further downstream analysis. The adoption of preparative LC into a workflow, with larger columns and instrumentation, can appear to be a daunting task. However, by following some fundamental guidelines and a little practical advice, it can be quite simple.</strong></p>
<h5>Key learning objectives</h5>
<ul>
<li><strong>Become familiar with the analytical to preparative chromatography workflow</strong></li>
<li><strong>Learn strategies to efficiently develop methods for purification success</strong></li>
<li><strong>Know how to avoid or recover from common mistakes</strong></li>
<li><strong>Understand how to think like a purification scientist</strong></li>
</ul>
<h5>Who should attend?</h5>
<ul>
<li>Analytical and medicinal chemists who want to learn more about purification or preparative LC, or regarding scale-up from analytical to preparative LC</li>
<li>Lab managers supporting purification labs</li>
<li>Scientists who are curious about incorporating purification into their laboratory workflow</li>
</ul>
<p> </p>
<blockquote>
<p><strong>Presenter:</strong> Scott Bollen (Business Development Manager EMEA, Agilent Technologies)</p>
</blockquote>
<blockquote>
<p>Scott specializes in small molecule LC columns, ranging from HPLC to UHPLC. Scott has worked at Agilent for 17 years. He started as a technical support manager, and now supports new LC products in R&amp;D as well as the entirety of the existing small molecule LC column portfolio in business development.</p>
</blockquote>


Agilent Technologies: Scale Up from Analytical to Prep with Agilent Prep LC Columns

Scale Up from Analytical to Prep with Agilent Prep LC Columns

Become familiar with the analytical to preparative chromatography workflow and learn strategies to efficiently develop methods for purification success.

From Analytical to Prep with Agilent Prep LC Columns

<p>Join us on 18 &amp; 19 January for two half days of virtual presentations as we help you to navigate the every day challenges faced with the analysis of oligonucleotides. Presented by experts in the industry and the Agilent biopharma team, these short talks are designed to educate and provide insight from basic oligonucleotide assessment, through to the more advanced applications. Q&amp;A sessions will follow each presentation to give you the opportunity to engage with our speakers.</p>
<p>The agenda will cover these topics below and more!</p>
<ul>
<li>Introduction to Oligonucleotide Therapeutics</li>
<li>Analytical &amp; Purification Methods</li>
<li>Workflows for Oligonucleotide CQAs</li>
<li>Practical Tips &amp; Tricks for Preparative HPLC</li>
<li>Analysis of mRNA 5&#39; Capping and Poly-A Tailing by High Resolution LC/MS</li>
</ul>
<h3><strong>Agenda Day 1</strong></h3>
<h5><strong>10:00  Welcome to Agilent Oligo Insight Days</strong></h5>
<h5><strong>10:10  Introduction to Oligonucleotide Therapeutics</strong></h5>
<h5><strong>10:45  Oligonucleotide Analytical and Purification Methods</strong></h5>
<h5><strong>11:25  Workflows for Analysis of Oligonucleotide CQAs</strong></h5>
<h5><strong>12:05  Oligonucleotide Analysis - Tips &amp; Tricks: Putting it all into Practice</strong></h5>


Agilent Technologies: Agilent Oligonucleotide Insight Days

Agilent Oligonucleotide Insight Days (Day 1)

Taking A Deeper Dive into Oligonucleotide Analysis

<p>Join us on 18 &amp; 19 January for two half days of virtual presentations as we help you to navigate the every day challenges faced with the analysis of oligonucleotides. Presented by experts in the industry and the Agilent biopharma team, these short talks are designed to educate and provide insight from basic oligonucleotide assessment, through to the more advanced applications. Q&amp;A sessions will follow each presentation to give you the opportunity to engage with our speakers.</p>
<p>The agenda will cover these topics below and more!</p>
<ul>
<li>Introduction to Oligonucleotide Therapeutics</li>
<li>Analytical &amp; Purification Methods</li>
<li>Workflows for Oligonucleotide CQAs</li>
<li>Practical Tips &amp; Tricks for Preparative HPLC</li>
<li>Analysis of mRNA 5&#39; Capping and Poly-A Tailing by High Resolution LC/MS</li>
</ul>
<h3><strong>Agenda Day 1</strong></h3>
<h5><strong>10:00  Welcome to Agilent Oligo Insight Days</strong></h5>
<h5><strong>10:10  A Beginners Guide to LC/MS for the Analysis of Oligonucleotides</strong></h5>
<h5><strong>10:45  Analysis of mRNA 5&#39; Capping and Poly-A Tailing by High-Resolution LC/MS</strong></h5>
<h5><strong>11:20  Nucleic Acids Therapeutics Delivery by LNPs: Fundamentals and Characterizations</strong></h5>
<h5><strong>12:05  Oligonucleotide Purification - Tips and Tricks How to Scale Up!</strong></h5>


Agilent Oligonucleotide Insight Days (Day 2)

<p><strong>It takes time, money, and resources to generate laboratory data, so any loss of data is a direct financial loss to your regulated company, as well as a major compliance risk. &nbsp;Laboratory data must be secured and available to support product quality decisions, both at the time of decision making and for the full duration of the mandated retention period.</strong></p><p>A regulated company must ensure its data is retained through the retention period and maintains its integrity during that time. &nbsp;While external assistance can be sought with activities such as backup and restore, disaster recovery and business continuity planning, archiving, system administration and cybersecurity, the overall responsibility for the regulated data remains with the regulated company.</p><p><strong>Key points of discussion will be:</strong></p><ul><li>Working with Servers</li><li>Having a Backup and Restore Plan</li><li>Developing a Disaster Recovery Plan</li><li>Have a Business Continuity Plan</li><li>Archiving Data</li><li>Cybersecurity Considerations</li><li>Updates to Software</li></ul><p>Please join us for a live discussion as we explore this topic. &nbsp;As always, don’t forget to bring your Empower questions for this exclusive opportunity to ask our experts anything about Empower Software.</p><blockquote><p><strong>Presenter: </strong>Neil Lander (Principal Product Manager, Informatics, Waters Corporation)</p><p>Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager focusing on Informatics solutions. He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.</p><p><strong>Presenter:&nbsp;</strong>Fiona O'Leary (Informatics Consultant, Waters Corporation)</p><p>Fiona is an experienced Empower Software and Data Integrity professional working within the EU Professional Services Team.</p><p>She has ~18 years of experience with Waters, beginning with Technical Support &amp; Empower Training, moving to Empower Consultancy and Workflow Optimization in more recent years. Her focus now is on building Data Integrity into the Lab workflow, Custom Field automation and Electronic Data Review / Reporting optimization. Prior to joining Waters, Fiona spent some years in the GxP Pharmaceutical Manufacturing world, setting the scene for her current goal of helping organizations achieve their scientific and operational objectives efficiently and within compliance guidelines. She graduated from Dublin City University in 2001 with a BSc Hons. Analytical Science.</p></blockquote>

Waters Corporation: Empower Tips Ask Me Anything About Durable Data: A Guide to Ensuring Your Data Is Secure and Available from Creation Thru the Full Mandated Retention Period

Empower Tips Ask Me Anything About Durable Data: A Guide to Ensuring Your Data Is Secure and Available from Creation Thru the Full Mandated Retention Period

Protecting lab data is critical for compliance and financial health. Learn key strategies for secure retention, recovery, and cybersecurity in regulated labs.