Dnešním hostem podcastu CHEmic je jeho dosavadní autorka Veronika Sedláčková – novinářka, moderátorka a odcházející tisková mluvčí ÚOCHB.&nbsp;Jak se jí po téměř třiceti letech v médiích přecházelo do světa vědecké komunikace? A co by vzkázala lidem, kteří chtějí o vědě mluvit a psát? Odpovědi na to zazní v epizodě, která uzavírá dosavadní sérii podcastu CHEmic.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Tomas_Bellon_Podcast_CH_Emic_76_Je_dulezite_nebat_se_ptat_rika_Veronika_Sedlackova_492ca19eb1.jpg" alt="ÚOCHB/Tomáš Belloň: Podcast CHEmic #76 – Je důležité nebát se ptát, říká Veronika Sedláčková" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Tomas_Bellon_Podcast_CH_Emic_76_Je_dulezite_nebat_se_ptat_rika_Veronika_Sedlackova_492ca19eb1.jpg 208w," sizes="100vw" width="1500" height="1125"><blockquote>Podcast CHEmic představuje vědce a vědkyně z Ústavu organické chemie a biochemie AV ČR, jejich život v labech i mimo ně.</blockquote><blockquote>CHEmic najdete v podcastových aplikacích:</blockquote><blockquote><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/0ELIQTRkC3TFrVYnZgztsy?si=b4407e1f54824315&amp;nd=1">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/cz/podcast/podcast-chemic-1-riskovat-se-vyplat%C3%AD-ne%C3%BAsp%C4%9Bchu-se-nesm%C3%ADme/id1683797089?i=1000611870620">Apple Podcasts</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.google.com/feed/aHR0cHM6Ly9hbmNob3IuZm0vcy9kZmFhNjA4OC9wb2RjYXN0L3Jzcw">Podcasty Google</a></li></ul></blockquote><blockquote>a na našem <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/playlist?list=PLFJNDHHAxyMszoY-Hm4BGJ6nN7zNLZXO-">YouTube kanálu</a>.</blockquote>

Podcast CHEmic #76 – Je důležité nebát se ptát, říká Veronika Sedláčková

<h4>S potěšením oznamujeme, že je nyní dostupná nová verze <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products/305">Clarity Chromatografického Softwaru</a> 10.1.2!</h4>Tato menší aktualizace rozšiřuje portfolio systému Clarity o nové moduly pro řízení přístrojů.<h5>Nově přidané moduly pro řízení přístrojů</h5><ul><li>pomocné moduly Ecom</li><li>ICF moduly pro HTA</li><li>čerpadlo Spark</li></ul><h5>Aktualizované moduly pro řízení přístrojů</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/companies/168">Ecom</a> HPLC</li><li>Knauer HPLC</li><li>Spark ICF moduly</li><li>YoungIn Chromass HPLC</li></ul><blockquote>Úplný seznam změn naleznete v dokumentu <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/version_history?product=1">Changelogu</a>. Pro více informací o nových funkcích v systému Clarity doporučujeme přehled novinek verze 10.1.Software Clarity dokáže <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/controls">řídit více než 1000 přístrojů</a>; jejich seznam je k dispozici online.</blockquote><h3>Podporované přístroje v Clarity (DataApex) – rychlý přehled</h3>Chromatografický software Clarity podporuje široké spektrum chromatografů a periferních zařízení prostřednictvím digitálního řízení (DD) a analogového sběru dat (A/D) pomocí modulu Colibrick.<h4>Plynové chromatografy (GC)</h4>Digitální integrace přes LAN/RS232 (mnohé přes ICF):<ul><li>Agilent: 5890, 6890, 7890, 8860, 8890, Intuvo 9000</li><li>Shimadzu: GC-2010/2014, Nexis GC-2030</li><li>Thermo Fisher: TRACE 1300/1310</li><li>PerkinElmer: Clarus 400–690</li></ul>Další podporované značky: Dani, Ellutia, Young In Chromass, Apix, Fuli, GOW-MAC (vybrané modely)<blockquote>Poznámka: Většina systémů Agilent 6890–8890 a Intuvo využívá LAN – ICF.</blockquote><h4>HPLC systémy</h4>Plné řízení systému nebo jednotlivých modulů:<ul><li>Agilent 1100–1290 Infinity II (ICF)</li><li>Shimadzu LC-10/20 a Nexera LC-40</li><li>PerkinElmer Flexar / NexSAR</li><li>Knauer Azura / BlueShadow / Smartline</li><li>Hitachi Chromaster / Primaide</li><li>Young In Chromass ChroZen</li><li>Sykam S500–S600, Watrex Streamline, Hanon LC7000–7300</li></ul><h4>HPLC moduly (čerpadla, detektory, termostaty kolon, frakční kolektory)</h4><ul><li>Čerpadla: Agilent 1100–1290, Knauer, Shimadzu, PerkinElmer, Sykam, SSI, Ecom</li><li>Detektory: VWD, DAD (PDA), RID, FLD, ELSD (Agilent, Shimadzu, Knauer, PerkinElmer, Young In Chromass, Ecom aj.)</li><li>Termostaty kolon: Agilent G1316x / G7116x, Shimadzu CTO-20/30/40, Knauer CT2.1, PerkinElmer Flexar</li><li>Frakční kolektory: Knauer FC 6.1, Teledyne FOXY, Ecom ECF 2096, Waters Fraction Collector II</li></ul><h4>Autosamplery</h4><ul><li>Agilent GC ALS (7673/7683/7693/8697), HPLC ALS moduly (G1313A–G7167C)</li><li>Shimadzu AOC-20 / AOC-5000+, SIL-10 / SIL-20 / SIL-40</li><li>CTC Analytics PAL, HTA autosamplery, Spark Alias/UHPLC, Knauer AS 6.1L</li><li>PerkinElmer Flexar / NexSAR</li></ul><h4>Další zařízení</h4><ul><li>GC×GC modulátory (např. GC×GC modulátor Univerzity Karlovy)</li><li>Ventily a přepínací moduly (Agilent, Knauer, Shimadzu, VICI-Valco)</li><li>Odplyňovače, průtokoměry, řídicí jednotky, rozhraní</li><li>LC-MS / GC-MS: Advion Expression CMS, Microsaic 4500 MiD, Markes BenchTOF aj.</li><li>Váhy (EA rozšíření): Mettler Toledo, Sartorius</li></ul><h4>Analogový sběr dat (Colibrick)</h4>Podpora A/D pro starší nebo analogové přístroje:<ul><li>GC: Agilent 5880/4890A, Varian 3xxx/3800/3900, PerkinElmer Clarus, Thermo TRACE, Shimadzu GC-14/9A, Scion 43x/45x/83xx/85xx aj.</li><li>HPLC detektory: Waters 2487/2489, ESA Corona, Hitachi L-series, JASCO FP/UV, Ecom LCD a další</li><li>Elementární a aminokyselinové analyzátory: Carlo Erba/Costech ECS, Biochrom 20/30+</li></ul>

DataApex Clarity chromatografický software verze 10.1.2 je nyní k dispozici!

V týdnu od 6. dubna na Vás čekají v oblasti kapalné fáze a především HPLC a LC/MS následující webináře.<blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://www.lcms.cz/webinars">👉 DETAILY A REGISTRACE V SEKCI WEBINÁŘE</a></h4></blockquote><blockquote>👉 Vyhledávejte a filtrujte v největší globální databázi s více než 5 500 GC, HPLC, MS a spektroskopickými webináři.</blockquote><h5>1. Waters Corporation: Fit-for-purpose workflows for inositol stereoisomer profiling in dietary supplements and foods using single and tandem mass spectrometry</h5><ul><li>Út, 7.4.2026 19:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6030">Registrace</a></li></ul>Webinář představí analytické postupy pro současné oddělení a kvantifikaci stereoisomerů inositolu v jednoduchých i komplexních matricích potravin a doplňků stravy.<h5>2. Thermo Fisher Scientific: Transforming Fuel Testing Performance with Combustion Ion Chromatography</h5><ul><li>St, 8.4.2026 07:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6093">Registrace</a></li></ul>Zjistěte, jak spalovací iontová chromatografie (C-IC) zlepšuje přesnost a efektivitu testování paliv v moderních laboratořích.<h5>3. SCIEX: Getting started with LC-MS/MS for PFAS</h5><ul><li>St, 8.4.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6102">Registrace</a></li></ul>Naučte se vytvářet LC-MS/MS metody pro PFAS, dosahovat ultra nízkých detekčních limitů a získat praktické zkušenosti pro environmentální a potravinářské aplikace.<h5>4. Phenomenex: Extraction Face-Off: Choosing Between SLE and SPE</h5><ul><li>St, 8.4.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6092">Registrace</a></li></ul>Pochopte rozdíly mezi SLE a SPE a naučte se zvolit správnou extrakční techniku pro vaši aplikaci.<h5>5. Waters Corporation: RADIAN™ ASAP Mass Spectrometry for the Detection and Analysis of Explosive Traces</h5><ul><li>St, 8.4.2026 16:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6031">Registrace</a></li></ul>Zjistěte, jak RADIAN™ ASAP umožňuje rychlou a spolehlivou detekci stopových množství výbušnin pomocí hmotnostní spektrometrie.<h5>6. Technology Networks: Label Free Quantitation: New Insights Into High-Throughput Proteomic Workflows</h5><ul><li>Čt, 9.4.2026 16:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/5969">Registrace</a></li></ul>Objevte pokročilé LC–MS workflow pro proteomiku bez značení (LFQ) s vysokou propustností a robustní reprodukovatelností.

Webináře LabRulezLCMS týden 15/2026

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Česká společnost DataApex se od svého založení v roce 1991 věnuje vývoji chromatografického softwaru. DataApex se neustále snaží poskytovat uživatelům efektivní a aktuální nástroje pro zpracování chromatografických dat.

DataApex

ÚOCHB AV ČR je přední mezinárodně uznávaná vědecká instituce, jejímž hlavním posláním je základní výzkum v oblasti chemické biologie a medicinální chemie, organické a materiálové chemie, chemie přírodních látek, biochemie a molekulární biologie, fyzikální chemie, teoretické chemie a analytické chemie.

Ústav organické chemie a biochemie AV ČR

Vše ze světa analytické chemie na jednom místě. Propojujeme lidi při řešení jejich problémů. U nás naleznete všechny potřebné informace snadno, rychle a přehledně. Přestaňte hledat a začněte nacházet.

LabRulez

Agilent Technologies

Technology Networks

SelectScience

Waters Corporation

<ul>
<li>Wait what?! Who knew!? There are so many things that a mass spec can do.
</li>
<li>I&#39;ve used it for years, yes this may be true.
</li>
<li>I&#39;ve processed some samples, more than a few.
</li>
<li>But between me and you, I had no idea all the things it could do!
</li>
<li>From sample prep to separation, you just won&#39;t believe…
</li>
<li>All of the tips and tricks we have up our sleeve.
</li>
<li>So, take a few minutes, about 15 should do.
</li>
<li>And learn all the things your mass spec can do.
</li>
</ul>
Thinking of developing a new method utilizing MS? Selecting the best analytical method begins with a decision on how to best separate the analytes of interest. This choice will affect sample preparation and sample introduction, so it is important to get it right early. In this webinar we will review analyte structural properties that make the compound more amenable to either liquid chromatography or to gas chromatography.
<blockquote>
Presenter: Karen Yannell (LC/MS Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Karen E. Yannell is a LC/MS Application Engineer at Agilent Technologies. She is an expert in small molecule high throughput analysis by triple quadrupole (TQ) and quadrupole time-of-flight (QTOF) mass spectrometry for applied markets. Her recent work has focused on developing routine Q-TOF workflows for both expert and non-expert users alike. Previously, Karen worked on forensic, metabolomics, and biomarker discovery applications with ambient ionization sources. She received her PhD in Analytical Chemistry from Purdue University where studied under the advisement of Prof. R. Graham Cooks. Outside of the laboratory, she enjoys cooking unique dishes and hiking along the beach with her dog.
</blockquote>
<blockquote>
Presenter: David Weil, PhD (Senior Applications Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
David Weil is a senior applications scientist in Agilent located in Wood Dale with over 30 years of experience using mass spectrometry. Prior to joining Agilent in 2004, he worked for 14 years at 3M in the Corporate Research Analytical Laboratories (St.Paul, MN) and 4 years at Nicolet/Extrel doing ultra-high resolution mass spectrometry. David received his Ph.D. in Physical Chemistry from the University of Minnesota and Post Doc with Dr Charles Wilkins at University of California-Riverside developing laser desorption FTMS and ion-molecule reactions. David has three grown sons and live with his wife Naomi just blocks from Wrigley Field and Lake Michigan.
</blockquote>
<blockquote>
Presenter: Matt Curtis, PhD (GC/MS Applications Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Matt joined Agilent in 2013 as a GC/MS applications scientist in the Santa Clara, CA Center of Excellence. His primary responsibility is focused on providing instrument demos for customer interested in each technology Agilent offers; the GC/MS single quadrupole, triple quadrupole and the quadrupole Time-of-Flight. The variety of different samples submitted to the demo lab provide a unique knowledge of optimizing parameters for different applications to provide the best quality data from the instrument.
</blockquote>

Agilent Technologies: Selecting your Separation: Are my Compounds Better for GC or LC

Selecting your Separation: Are my Compounds Better for GC or LC

We will review analyte structural properties that make the compound more amenable to either liquid chromatography or to gas chromatography.

Separation selection: Are my Compounds Better for GC or LC

TBD
<blockquote>
Presenter: Mark Sartain, Ph.D. (LC/MS Applications Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark has developed a diverse array of targeted and nontargeted mass-spectrometry based metabolomics and lipidomics applications since joining Agilent in 2012, with a focus on complete hardware/software workflows. Mark began utilizing mass spectrometry in his graduate work to characterize glycoproteins, and later established a novel LC/MS strategy to comprehensively profile and identify complex lipids of Mycobacterium tuberculosis in his postdoctoral fellowship at Colorado State University. He continued designing a wide range of MS-based techniques to measure metabolites and lipids for diverse projects as a Research Scientist at the Institute of Systems Biology (Seattle, WA) prior to joining Agilent.
</blockquote>

Agilent Technologies: LC/TQ Solutions to Give Your Lab Multi-omic Biological Insights

LC/TQ Solutions to Give Your Lab Multi-omic Biological Insights

LC/TQ to Give Your Lab Multi-omic Biological Insights

High resolution mass spectrometry (HRMS) has gained importance for broad screening in forensic toxicology laboratories with the emergence and constant evolution of novel psychoactive substances.
The Xevo G3 QTof from Waters is the latest generation of high performance QTof technology and includes many enhancements that improve performance and system robustness. 
Among others, ion optics have been improved to enhance the detection of labile drugs such as amphetamines resulting in significantly higher sensitivity. This presentation aims to highlight some of the developments and enhancements that have been made to the recently launched Xevo G3 QTof.
Also detailed in the presentation will be a comparison study conducted between the outgoing Xevo G2-XS QTof with the Xevo G3 QTof, by means of system setup, data generated e.g., richness of information e.g., detection accuracy and analytical sensitivity.
Learning objectives – what you will learn: 
<ul>
<li>Fundamentals of high resolution mass spectrometry</li>
<li>Enhancements made to the recently launched Xevo G3</li>
<li>QTof Comparison data discussion</li>
</ul>
<blockquote>
Presenter: Nayan. S. Mistry (Senior Application Scientist, Forensics, Research &amp; Development, Waters Corporation, Waters Corporation)
</blockquote>
<blockquote>
Nayan Mistry is a Senior Application Scientist at Waters Corporation. Nayan joined Waters in 2009 and is part of a global team dedicated to performing research and development of applications and analytical solutions for Forensic Toxicology. 
</blockquote>
<blockquote>
Nayan’s key focus is on toxicology screening and he has expertise with both nominal mass and accurate mass-based screening using UNIFI and Waters_Connect application software. Nayan plays a key role in the development of these solutions and in the expansion of associated libraries. Currently Nayan is playing a key role in the development and support of the recently launched Xevo G3 QTof mass spectrometer in addition to other responsibilities for the nominal mass applications. His favourite aspect of the role is customer support - Nayan is greatly involved in global on-site customer support and training and has presented his work at conferences worldwide.
</blockquote>

Waters Corporation: High Resolution MSE Toxicology Screening using the Waters Xevo G3 QTof

High Resolution MSE Toxicology Screening using the Waters Xevo G3 QTof

This presentation aims to highlight some of the developments and enhancements that have been made to the recently launched Xevo G3 QTof.

HR MSE Toxicology Screening using the Waters Xevo G3 QTof

Purification is critical to many scientific workflows. Compounds of interest are rarely found to be the right purity in their native state. Join Waters and Virscidian for this 6-part webinar series to learn the basics of purification and application of purification principles for more advanced workflows.
<h3>PART 4 | Using the Waters Fraction Manager Analytical (WFMA) to Achieve UPLC Scale Purification</h3>
Analytical scale purification provides for routine and robust isolation of your investigatory compounds. Challenges with UPLC purification include peak widths of 1-3 seconds and collecting the 6 to 50 µL peak volume. The Waters Fraction Manager Analytical (WFMA) addresses these challenges. Learn more about analytical scale purification with Waters. 
Learning Objectives: 
<ul>
<li>Understand analytical scale purification</li>
<li>Implement best practices to collect small volume fractions</li>
</ul>
<blockquote>
Presenter: Andrew Leightner (Market Development Manager – Americas Field Marketing, Waters Corporation)
</blockquote>
<blockquote>
Andy Leightner has over 10 years of experience serving the pharma market from contract research organizations. He specialized in method development and validation leveraging LC, GC, and mass spectrometry to meet complex client needs. He supports pharmaceutical and biopharmaceutical discovery and development in the Americas for Waters Corporation. Andy received a bachelors degree in biology from the University of Wisconsin-Madison.
</blockquote>

Waters Corporation: Using the Waters Fraction Manager Analytical (WFMA) to Achieve UPLC Scale Purification

Using the Waters Fraction Manager Analytical (WFMA) to Achieve UPLC Scale Purification

Learn more about analytical scale purification with Waters.

Waters Fraction Manager Analytical UPLC Scale Purification

This webinar we will help you to understand more about the latest advancements in sample preparation strategies to enable accurate PFAS determination in challenging food matrices.
PFAS bioaccumulate within in the environment and ultimately manifest as PFAS contamination in the food chain. In recent years, significant contamination has been reported in meat, fish, dairy produce fruit, vegetables as well as processed foods.
Even when sophisticated chromatographic separation and mass detection technologies are employed for their analysis, these varied and complex matrices present many challenges for accurate PFAS determination.
Key learning objectives
<ul>
<li>Attendees will learn the latest advancements in sample preparation techniques for difficult food matrices for the analysis of PFAS</li>
<li>Our sample preparation solutions for PFAS analysis have been verified and validated</li>
<li>Attendees will be equipped with a workflow solutions they can implement right away</li>
</ul>
Who should attend?
This is suitable for anyone who is working in a laboratory environment preparing environmental or food samples for PFAS analysis. However, we welcome anybody who would like to simply learn more about sample preparation of food matrices for PFAS analysis.
<blockquote>
Presenter: Nassima Galloway (Business Development Manager Food &amp; Environmental, Agilent Technologies)
</blockquote>
<blockquote>
Nassima Galloway BSc obtained her degree at Manchester University in Medical Biochemistry BSc. Her professional career began working with environmental laboratories within EMEA offering support on sample preparation solutions and helping laboratories to optimize their workflows and throughout.
</blockquote>
<blockquote>
She now holds the position of Business Development Manager for EMEAI within the chemistries supplies division of Agilent Technologies. Nassima works closely within the food &amp; environmental markets to promote the latest advancements in sample preparation techniques and workflow solutions for LC/MS GC/MS.
</blockquote>
<blockquote>
Presenter: Carola Damen (LC/MS Product Specialist Benelux, Agilent Technologies)
</blockquote>
<blockquote>
Carola Damen obtained her master&#39;s degree in pharmacy at the University of Groningen, after which she completed her pharmacist training at the same university. During her studies she specialized fully in the field of analytical chemistry and in particular liquid chromatography coupled with mass spectrometry.
</blockquote>
<blockquote>
Carola obtained her PhD at Utrecht University, where she carried out this PhD program at the Netherlands Cancer Institute in Amsterdam. The research focused on bioanalysis of anti-cancer drugs using LC/MS. After having fulfilled various laboratory functions, Carola has been working as a product specialist LC/MS at Agilent since 2020.
</blockquote>

Agilent Technologies: Analysis of PFAS in the Food Chain

Analysis of PFAS in the Food Chain

Understand more about the latest advancements in sample preparation strategies to enable accurate PFAS determination in challenging food matrices.

Human shelterin is a six-subunit complex that binds telomeres, protects them from the DNA-damage response, and regulates the maintenance of telomeric DNA.
Although high-resolution structures have been generated for individual structured domains of shelterin subunits, the structure and stoichiometry of the full complex is unknown.
In this webinar, Dr. John Zinder, a Senior Scientist in the Structural Biology group at Odyssey Therapeutics, will discuss the purification of shelterin subcomplexes and reconstitution of the entire complex using full-length, recombinant subunits. By combining mass photometry, negative-stain electron microscopy (EM), crosslinking mass spectrometry (XLMS), AlphaFold modelling, and native mass spectrometry (MS), stoichiometries were obtained, as well as domain-scale architectures of shelterin subcomplexes.
In addition, Dr. Zinder will present data on shelterin complexes which feature extensive conformational heterogeneity, regardless of the presence of DNA substrates. He will also explain how shelterin adopts a multitude of conformations and its unusually high structural variability can be beneficial for its many functions at telomeres.
Key learning objectives
<ul>
<li>Learn how to study conformational heterogeneity using single-particle mass analysis, including mass photometry</li>
<li>Understand how modelling can aid in the characterization of highly flexible assemblies, such as shelterin</li>
<li>Discover how mass photometry, especially when combined with complementary techniques, can be a powerful tool for determining the stoichiometry of a complex as well as its compositional heterogeneity at low concentrations</li>
</ul>
Who should attend?
<ul>
<li>Anyone interested in mass photometry, the architecture of shelterin, protein complexes, protein-DNA interactions, telomere stability, structure determination, molecule prediction, homology modeling, and mass spectrometry.</li>
</ul>
<blockquote>
Presenter: Davide Zinder PhD (Odyssey Therapeutics)
</blockquote>
<blockquote>
Dr. John Zinder is a Senior Scientist in the Structural Biology group at Odyssey Therapeutics and works in the development of novel therapeutics. He has an undergraduate degree in Chemistry from Cornell University, and a masters degree in Biochemistry from the University of Colorado. He was awarded a PhD in Chemical Biology from his research in Chris Lima’s lab at the Sloan Kettering Institute. Zinder subsequently became a Damon Runyon Postdoctoral Fellow in the de Lange and Walz labs at the Rockefeller University.
</blockquote>
<blockquote>
Presenter: Lawrence Howes (SelectScience)
</blockquote>
<blockquote>
Lawrence studied biological sciences at Swansea University, UK, before completing a master’s in epidemiology at the University of Bristol. As a member of the Editorial team, he now plays an integral role in shaping the content on SelectScience
</blockquote>

SelectScience: Architecture and Stoichiometry of Human Shelterin Using Single-Particle EM and Mass Analysis

Architecture and Stoichiometry of Human Shelterin Using Single-Particle EM and Mass Analysis

Purification of shelterin subcomplexes and reconstitution of the entire complex using full-length, recombinant subunits.

Architecture and Stoichiometry of Human Shelterin

Adsorption, distribution, metabolism and excretion (ADME) profiling is used to determine the properties of drug candidates. Modern drug discovery environments depend on the accuracy and precision of results from ADME studies to select new targets with a high potential for clinical success.
However, as technology advances, the size of compound libraries increases. Once a compound shows an affinity for a target, understanding the ADME profile of a drug in vitro can reduce attrition rates in the drug development process.
Acoustic ejection mass spectrometry (AEMS) has a significantly higher throughput than traditional LC-MS approaches due to the rapid sampling and flow injection analysis enabled by a high-sensitivity mass spectrometer.
In the webinar, we discuss a suite of metabolic stability assays developed using an AEMS system.
Attend this webinar to: 
Learn about how the Echo® MS system can be used in drug discovery 
Hear about the advantages of AEMS 
Discover the fundamentals of Echo® MS operation and how it can benefit your research
<blockquote>
Presenter: Dr. Jacob W. McCabe (Senior Scientist Echo® MS Center of Excellence, SCIEX)
</blockquote>

Technology Networks: Discover New Ways To Increase Your Throughput for Metabolic Stability Assays

Discover New Ways To Increase Your Throughput for Metabolic Stability Assays

In the webinar, we discuss a suite of metabolic stability assays developed using an AEMS system.

Increase Your Throughput for Metabolic Stability Assays

L-asparaginase (ASNase) is an enzyme-drug that is approved for the treatment of pediatric acute lymphoblastic leukemia (ALL) but is too toxic for adults. To elucidate potentially targetable mechanisms of toxicity with the ultimate goal of decreasing ASNase toxicity and extending its use to adults, we performed multi-omic profiling of the response to ASNase in adult (16-week-old) mice. We administered a range of sub-toxic and toxic doses of ASNase to healthy mice, collected whole blood samples, processed them to plasma, and used a novel sample preparation with just 20 µL mouse plasma as input. We analyzed the extracts using a multi-omic, bioanalytical LC/MS platform capable of measuring 500+ metabolites, 750+ lipids, and 375 peptides.
A Bio-LC was used with three different separations for metabolites, lipids, and peptides. A fast, sensitive, robust, 6495 LC/TQ MS system was used with comprehensive databases for pathway analysis. The results suggested that ASNase induces multi-modal cell death as evidenced by metabolite-, lipid-, and peptide/protein-level changes that occurred in a dose-dependent manner. 
Future studies will test the hypothesis that inhibiting those modes of cell death will decrease ASNase toxicity while preserving anticancer activity. Overall, our findings provide proof-of-concept that multi-omic analysis of drug mechanisms at the protein-, lipid-, and metabolite-levels can unveil toxicity-associated pathways that can be targeted to potentially improve a drug’s therapeutic index.
<blockquote>
Presenter: Ian Edwards (LC/TQ Product Manager, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Ian holds a Ph.D. from the Centre for Proteome Research at the University of Liverpool.He has spent time as a pre- and post-sales MS applications specialist focused on MS imaging and proteomics, as a field marketing manager for -omics, and as a global pharmaceutical marketing manager. Ian joined Agilent recently as LC/TQ Product Manager.
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Presenter: Philip Lorenzi, Ph.D. (Associate Professor, Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center)
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<blockquote>
I’m an Associate Professor in the Department of Bioinformatics and Computational Biology at The University of Texas MD Anderson Cancer Center. My laboratory focuses on the development of metabolic therapies for the treatment of cancer (with an emphasis on nutrient metabolism), and I’m also Director of the Proteomics Core Facility and Director of the Metabolomics Core Facility. I’ve co-authored 71 peer-reviewed publications. I co-wrote and was Co-Investigator on a successfully completed $4.5M CPRIT Core Facility Award, managed staff and the budget, and oversaw development of bioanalytical services using current equipment and protocols that we have optimized thoroughly. We’ve developed novel mass spectrometry-based methods for: 1) increasing the accuracy of glutamine and glutamate measurements by minimizing in-source conversion to pyroglutamate; 2) normalizing data based on the DNA content of cell- and tissue-based samples; 3) measuring amino acids from as little as 2 µL of mouse plasma or whole blood; 4) detection of over 2,000 annotated metabolites and lipids per biological sample (including just 2 mg of tissue); 5) stable isotope tracer analyses in vitro and in vivo including methods for measuring energy metabolism; 6) determining the extent and position of 13C incorporation into specific amino acids; and 7) diagnosing and correcting batch effects in metabolomic data. Our multidisciplinary team provides full service contract research in a state-of-the art facility.
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Agilent Technologies: Multi-Omic, Mass Spectrometry-Based Elucidation of Mech. of L-asparaginase Toxicity in a Mouse Model

Multi-Omic, Mass Spectrometry-Based Elucidation of Mech. of L-asparaginase Toxicity in a Mouse Model

We analyzed the extracts using a multi-omic, bioanalytical LC/MS platform capable of measuring 500+ metabolites, 750+ lipids, and 375 peptides.

Multi-Omic, Mass Spectrometry-Based Elucidation of Mech

How about a 20 minute coffee break along with a bite of Empower? Here you go!
Empower custom fields and Excel in Empower LMS - not a conflict of interest?
Excel Compliance is designed as the solution for customers operating in a regulated environment to use excel spreadsheet with confidence. It provides full audit trail that is compliant to regulatory requirements for electronic records and signatures. 
<ul>
<li>Mitigate the compliance risk of traditional uncontrolled spreadsheets and documents
</li>
<li>Enhanced compliance features such as:
<ul>
<li>authorized access control
</li>
<li>automated version control
</li>
<li>document level and cell by cell audit trails
</li>
</ul>
</li>
<li>Centralize and secure laboratory spreadsheets and documents for use in a regulated environment
</li>
</ul>
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Presenter: Ralf Schröder (Informatics Product Marketing Europe·Waters GesmbH)
</blockquote>

Waters Corporation: Excel and Compliance in Empower LMS

Excel and Compliance in Empower LMS

Empower custom fields and Excel in Empower LMS

Examining powdered samples in the presence of a reactive gas or at elevated temperatures could be a difficult measurement to make.
During this workshop, we will be looking at how to make such diffuse reflection measurements using a high-end Cary instrument and an accessory from Harrick Scientific known as the Praying Mantis. Tips and tricks of using this accessory will also be discussed.
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Presenter: Mark Fisher, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)
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<blockquote>
Mark, an Application Scientist for Agilent Technologies, has over 35 years of experience in molecular spectroscopy. He has held roles in Agilent&#39;s UV-Vis-NIR, fluorescence, and atomic absorption product portfolios. In addition to identifying industry trends and new business needs, Mark&#39;s responsibilities include application development, pre and post-sales support, as well as acting as a customer advocate for new product and technology opportunities. Mark received his doctorate in analytical chemistry from the University of Nebraska-Lincoln.
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<blockquote>
Presenter: Sue Berets, PhD (Applications Scientist, Harrick Scientific Products)
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<blockquote>
Sue, an Applications Chemist for Harrick Scientific Products, has over 30 years of experience in accessories and optics for molecular spectroscopy. Sue received her doctorate in physical chemistry from Tufts University and has carried out basic research in theoretical and applied spectroscopy. Her responsibilities include technical and sales support, including accessing products for specific customer applications. She is involved in new product development, from initial concept to finished product, to meet the every changing applications needs of the market.
</blockquote>

Agilent Technologies: Unique Measurement Capabilities Using the Praying Mantis and a Cary UV-Vis system

Unique Measurement Capabilities Using the Praying Mantis and a Cary UV-Vis system

How to make such diffuse reflection measurements using a high-end Cary instrument and an accessory from Harrick Scientific known as the Praying Mantis.