Achieving Your Sustainability Goals with UPLC

Join us for our 3-Part series to Meet our Experts and discuss topics including:

• History of HPLC column technology over the last 50 years and how advancements have helped address current separation challenges.

• Fundamentals of small molecule separations to enable you to develop methods more efficiently.

• Solvent and column choices with UPLC to develop more environmentally friendly chromatography methods

PART 3: Achieving Your Sustainability Goals with UPLC

Method development for reversed-phase high-performance liquid chromatography (HPLC) separations has traditionally been conducted using acetonitrile-water or methanol-water gradients. Acetonitrile is often preferred because of its ability to dissolve a wide range of solutes, its low UV cutoff, compatibility with mass spectrometry detection, and lower viscosity. However, its use can also be problematic due to health and environmental concerns. This presentation will highlight how to reduce the use of acetonitrile while maintaining separation performance by using narrower ID columns containing smaller particles. Chromatography using alternatives to acetonitrile such as isopropanol or ethanol will be shown utilizing high-pressure LC instrumentation. Both techniques could be employed when developing more environmentally friendly LC methods.

Presenter: Bonnie Alden (Principal Scientist, Research & Development, Waters Corporation)

Bonnie Alden joined Waters Corporation in 1982 to work with Dr. Patrick McDonald to develop new Sep-Pak Cartridge SPE devices. During her career, she has been a member of teams that brought transformative chromatographic columns to market. These include Symmetry Columns which set the industry standard in reproducibility, 1st hybrid chromatographic packing of XTerra Columns, 2nd generation Ethylene Bridged Hybrid (BEH) and Charged Surface Hybrid (CSH) technologies, as well as the recent development of modern, mixed-mode stationary phases for challenging analyses, such as the retention and separation of polar acids. More than 35 years later, she is still an enthusiastic student of liquid chromatography and separation science. Bonnie is currently a principal scientist in the Small Molecule Portfolio Team where her research interests have focused on developing products to address the needs of scientists in the extensive field of small molecule drug development.