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Pushing the Limits of Bottom-Up Proteomics with State-Of-The-Art Capillary UHPLC and Orbitrap Mass Spectrometry for Reproducible Quantitation of Proteomes

 

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LC-MS: Vanquish Neo UHPLC system sets new performance standards for single-shot nanoLCMS bottom-up proteomics
LC-MS TECHNICAL NOTE Vanquish Neo UHPLC system sets new performance standards for single-shot nanoLCMS bottom-up proteomics Authors biomarker discovery and precision medicine. The enormous variety and dynamic range of proteins found in biofluids such Runsheng Zheng , Christopher Pynn ,…
Klíčová slova
neo, neonanolcms, nanolcmsvanquish, vanquishpepmap, pepmapwash, washcolumn, columnproteomics, proteomicssystem, systemuhplc, uhplcshot, shotloading, loadingrates, ratesouter, outerspray, spraybottom
A novel 75 cm column size gives increased resolution and better sequence coverage
APPLICATION NOTE 21550 A novel 75 cm column size gives increased resolution and better sequence coverage Authors Goal Jon Ferguson*, Brian King, Mike Baynham Thermo Fisher Scientific, Runcorn, UK *Thermo Fisher Scientific, West Palm Beach, FL, USA To demonstrate the…
Klíčová slova
prtc, prtchela, helapeptide, peptidepeptides, peptidespwhh, pwhhgradient, gradientdigest, digestmass, massmin, minexactive, exactivepeak, peakuhplc, uhplcnanoflow, nanoflowaverage, averageorbitrap
Improvements in LFQ for reproducible quantification of proteomic experiments: how DDA outperforms DIA
Poster Note 64785 Improvements in LFQ for reproducible quantification of proteomic experiments: how DDA outperforms DIA Improvements in LFQ for reproducible quantification Ignacio Ortea1, Romain Huguet2, David Horn2, Andreas FR Huhmer2, and Daniel Lopez-Ferrer2 IMIBIC, Cordoba, Spain, 2 Thermo Fisher…
Klíčová slova
dia, diadda, ddalfq, lfqpeptides, peptidesexactive, exactivelabel, labelminora, minoraoutperforms, outperformsidentifications, identificationsnanocolum, nanocolumhram, hramquantification, quantificationdata, datafree, freelately
IMSC: Improvements in LFQ for reproducible quantification of proteomic experiments: how DDA outperform DIA
advanced label free quantitation software. In this work we compare HRAM quadrupole-Orbitrap™ DDA, and HRAM quadrupole-Orbitrap DIA methods head-to- DATA DEPENDENT advanced label free quantitation software. In this work we compare HRAM head to evaluate the sensitivity and number of…
Klíčová slova
dia, diadda, ddalfq, lfqexactive, exactiveoutperforms, outperformspeptides, peptidesminora, minoradata, datacolumn, columndependent, dependenthram, hramhuhmer, huhmerlopez, lopezmapper, mapperhorn
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LabRulez s.r.o. Všechna práva vyhrazena. Obsah dostupný pod licencí CC BY-SA 4.0 Uveďte původ-Zachovejte licenci.