<p><strong>Vědci z Ústavu organické chemie a biochemie AV ČR pronikají do detailů genové transkripce. Popsali dosud neznámý molekulární mechanismus, kterým může začínat přepis genetické informace z deoxyribonukleové kyseliny (DNA) do ribonukleové kyseliny (RNA). Zaměřili se na zvláštní molekuly nazývané alarmony, které se vyskytují v buňkách napříč organismy a jejichž hladina se často zvyšuje při buněčném stresu. Výsledky výzkumu zveřejnil prestižní vědecký časopis </strong><i><strong>Nature Chemical Biology</strong></i><strong>.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="ÚOCHB/Tomáš Belloň: Výzkum z ÚOCHB odhaluje dosud nepoznaný způsob přepisu genetické informace: Dr. Hana Cahová, vedoucí vědecké skupiny Chemická biologie nukleových kyselin." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>RNA molekuly mohou nést na jednom konci různé chemické modifikace, označované jako tzv. „čepičky“. U eukaryotních organismů, kam patří i lidské buňky, je nejznámější čepička, která se podílí na stabilitě RNA a regulaci jejího dalšího osudu. V posledních letech se však ukazuje, že existují i alternativní, nekanonické RNA čepičky, jejichž vznik a mechanismus připojení k RNA se zatím vymyká lidským znalostem. Mezi ně patří například tzv. alarmonové čepičky, tvořené molekulami dinukleosidpolyfosfátu, alarmonů, které chrání buněčnou RNA ve chvílích, kdy je buňka v ohrožení.</p><p>Tým <a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/cs"><strong>Dr. Hany Cahové</strong></a> se ve své studii zaměřil na RNA polymerázu z bakterií a sledoval, jakým způsobem může tento enzym zahájit transkripci za využití dinukleosidpolyfosfátů (NpNs) místo běžných stavebních bloků RNA. Vědci vůbec poprvé popsali na atomární úrovni, jak může vznikat RNA s alarmonovou čepičkou přímo při zahájení přepisu genu. Navíc pozorovali, že se Np<sub>n</sub>Ns vážou jiným typem párování, než je obvyklé.</p><p>Na práci se zásadně podílely <strong>Valentina Serianni z týmu Hany Cahové</strong>, která prokázala schopnost dinukleosidpolyfosfátů zahajovat přepis genu, a<strong> Jana Škerlová</strong>, která se zaměřila na strukturální analýzu RNA polymerázy a na základě dat z kryogenní elektronové mikroskopie ukázala, jak se molekuly dinukleosidpolyfosfátů váží do aktivního místa RNA polymerázy, tedy do srdce enzymu, kde se genetická informace přepisuje. Jejich studie pomáhá odhalovat procesy, které provázejí přepis genetické informace z DNA do RNA.</p><p>Hana Cahová k tomu dodává: <i>„Popisujeme něco, co se v buňkách skutečně děje a co nyní dokážeme pozorovat přímo na úrovni jednotlivých molekul. Získáváme tak odpovědi na zásadní otázky týkající se buněčných procesů, například jak se buňky přizpůsobují stresu. Právě RNA totiž přenáší kaskádu informací, které stojí za jakoukoli buněčnou reakcí, např. na stav ohrožení způsobený nedostatkem živin či teplotním šokem.“</i></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="ÚOCHB/Tomáš Belloň: Výzkum z ÚOCHB odhaluje dosud nepoznaný způsob přepisu genetické informace: Dr. Tomáš Kouba, vedoucí core facillity skupiny Kryogenní elektronová mikroskopie." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>Klíčovou roli v projektu sehrála kryogenní elektronová mikroskopie (cryo-EM), kterou ved<strong>l Dr. Tomáš Kouba</strong>, jeden z autorů článku. <i>„Kryoelektronová mikroskopie umožňuje zmrazit biologické molekuly skoro v jejich přirozené formě a následné získat trojrozměrnou strukturu takových molekul. Díky tomu můžeme nahlédnout přímo do aktivního centra enzymů a sledovat jejich fungování až na atomární úrovni,“ </i>vysvětluje Tomáš Kouba.</p><p>ÚOCHB vloni otevřel nové<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/cs"><strong>centrum cryo-EM</strong></a>, které je v českém prostředí unikátní. Špičkové kryogenní elektronové mikroskopy našly zázemí ve speciálně navržené budově a umožňují studium biologických procesů s mimořádnou strukturální přesností.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="ÚOCHB: Výzkum z ÚOCHB odhaluje dosud nepoznaný způsob přepisu genetické informace: Tato práce ukazuje, jak malé molekuly NpnN pomáhají bakteriím zahájit přepis genetické informace. Detailní zobrazení pomocí kryogenní elektronové mikroskopie odhaluje, jak se vážou na genetickou šablonu a fungují jako nekanonické „čepičky“ RNA." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"></p><blockquote><h4><strong>Původní článek</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5#citeas"><strong>Molekulární vhled do 5′ zakončení RNA pomocí Np</strong><i><strong><sub>n</sub></strong></i><strong>Ns zprostředkovaného bakteriální RNA polymerázou</strong></a></h4><p>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;</p><p><strong>Nat. Chem. Biol.</strong> <strong>21</strong> (2026)</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a></p><h6>licencováno pod <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstrakt</strong></h4><p>RNA zakončená dinukleosidovými polyfosfáty byla u bakterií i eukaryot objevena teprve nedávno. Pravděpodobný mechanismus tohoto specifického typu zakončení RNA spočívá v přímém začlenění dinukleosidových polyfosfátů RNA polymerázou jako nekanonických iniciačních nukleotidů. Dosud však nebylo známo, jakým způsobem se tyto sloučeniny vážou do aktivního místa RNA polymerázy během zahájení transkripce. V této práci jsme in vitro studovali iniciaci transkripce za použití série DNA templátů v kombinaci s dinukleosidovými polyfosfáty a modelovou RNA polymerázou z bakterie <i>Thermus thermophilus</i>. Zjistili jsme, že místo zahájení transkripce se může lišit v závislosti na kompatibilitě konkrétního templátu a použitého dinukleosidového polyfosfátu. Struktury iniciačních transkripčních komplexů s dinukleosidovými polyfosfáty získané pomocí kryogenní elektronové mikroskopie odhalily, že obě nukleové báze v molekule mohou párovat s DNA templátem. První kódovaný nukleotid se páruje kanonickým Watsonovým–Crickovým způsobem, zatímco druhá nukleová báze se váže nekanonicky prostřednictvím reverzního Watsonova–Crickova párování. Tato práce tak poskytuje strukturní vysvětlení mechanismu, kterým dinukleosidové polyfosfáty zahajují transkripci RNA.</p></div></div>

Výzkum z ÚOCHB odhaluje dosud nepoznaný způsob přepisu genetické informace

<p><strong>Falšování potravin je fenomén s dlouhou historií, který v dnešní době díky globalizaci potravinových řetězců a vysokým ekonomickým tlakům nabývá na významu. Ať už jde o ředění olivového oleje levnými oleji, přidávání cukru do medu, nebo skryté používání náhražek v bílkovinových doplňcích, výsledkem je ztráta důvěry spotřebitelů a v některých případech i ohrožení zdraví. Analytická chemie zde hraje klíčovou roli: poskytuje metody a technologie, které dokážou identifikovat a kvantifikovat cílové látky i nechtěné impurity v rozsáhlém spektru potravinových matrik.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg" alt="Watrex Praha: Falšování potravin a jak ho odhalí analytická chemie" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg 245w," sizes="100vw" width="1600" height="900"></p><h4><strong>Role chromatografie ve forenzní potravinářské analýze</strong></h4><p>Chromatografie, zejména kapalinová chromatografie (HPLC), je mezi analytickými metodami jedním z nejefektivnějších nástrojů pro separaci komplexních směsí molekul.</p><p><strong>Umožňuje:</strong></p><ul><li>kvalitativní identifikaci jednotlivých složek ve vzorku,</li><li>kvantitativní stanovení jejich koncentrací,</li><li>detekci nečistot a nečekaných složek, které mohou signalizovat falšování.</li></ul><p>V praxi se často kombinuje HPLC s různými typy detektorů (UV/VIS, DAD, refraktometrické detektory) nebo dalšími technikami (např. hmotnostní spektrometrií) pro zvýšení selektivity a citlivosti.</p><h4><strong>Izokratické vs. gradientní eluce</strong></h4><p>Při analýze potravinových vzorků se chromatografické metody volí podle cíle analýzy:</p><ul><li><strong>Izokratická HPLC</strong> používá jednu konstantní mobilní fázi během celé analýzy – je jednoduchá, robustní a ideální pro rutinní rutiny s dobře definovanými cílovými analyty (např. stanovení cukrů nebo vitamínů).</li><li><strong>Gradientní HPLC</strong> mění složení mobilní fáze během separace – umožňuje lepší rozlišení složitých směsí s širokým rozsahem polarity látek (např. peptidů nebo<br>fenolických sloučenin).</li></ul><p>Gradientní metody jsou obzvláště užitečné, když analyty mají velmi rozdílné vlastnosti a je potřeba dosáhnout vysoké účinnosti separace.</p><h4><strong>Watrex Praha a přínos moderních systémů pro potravinářskou analytiku</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy"><strong>Watrex Praha, s.r.o.</strong></a> patří mezi významné české výrobce a distributory systémů a přístrojů pro kapalinovou chromatografii, s dlouhou tradicí sahající do roku 1990. Firma úspěšně transformovala svou roli z distributora na výrobce vlastních HPLC a příbuzných technologií, přičemž klade důraz na modulární systémy robustní konstrukce vhodné i pro náročné analytické úlohy.</p><p>Jejich <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products?manufacturerItems=93&amp;search=streamline"><strong>řada Streamline™</strong></a> zahrnuje několik koncepcí HPLC systémů vhodných pro potravinářské aplikace:</p><ul><li><strong>Izokratický HPLC systém</strong> – jednoduchá, ale přesná konfigurace s jedním čerpadlem, kolonovým termostatem a UV/VIS detektorem; vhodný pro rutinní stanovení známých analytů (např. základní sacharidy, přídatné látky).</li><li><strong>Binární gradientní HPLC systém</strong> – disponuje dvěma čerpadly a umožňuje tvorbu gradientů mobilní fáze, což zvyšuje separační schopnost při analýze komplexních směsí (např. fenolické profily vína, složky aromatických extraktů).</li><li><strong>GPC/SEC systém (gelová permeační chromatografie)</strong> – specializované řešení pro stanovení makromolekul a polymerů, avšak i relevantní pro analýzu vysokomolekulárních složek v potravinách (např. polysacharidů).</li></ul><blockquote><p class="cke-user_style-important"><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Watrex_catalog_2024_ba97395f92.pdf"><strong>Produktový katalog ZDE</strong></a></p></blockquote><p>Díky modulární povaze těchto systémů mohou analytici snadno přizpůsobit konfiguraci specifickému analytickému problému, od jednoduchých rutinních analýz až po komplexní charakterizace potravinových vzorků.</p><h4><strong>Praktické příklady falšování odhalené HPLC</strong></h4><ul><li><strong>Detekce ředění olivového oleje: </strong>Pomocí HPLC lze separovat a kvantifikovat charakteristické triacylglyceroly a mastné kyseliny; neobvyklé vzory pak ukazují na příměsi levnějších olejů.</li><li><strong>Odhad přídavku cukru v medu: </strong>Izokratické HPLC s refraktometrickým detektorem může stanovit profily cukrů (glukóza, fruktóza, sacharóza), které ukazují na ředění nebo přidání cukerných sirupů.</li><li><strong>Identifikace aromatických adulterantů ve víně: </strong>Gradientní HPLC s PDA detektorem odhalí fenolické a aromatické profily, které mohou být narušeny přídavkem syntetických látek.</li></ul><h4><strong>Závěr</strong></h4><p>Falšování potravin je stále aktuálním problémem, který vyžaduje spolehlivé a citlivé analytické metody. Kapalinová chromatografie a zejména moderní HPLC systémy , představují jeden z nejúčinnějších nástrojů pro odhalování nežádoucích přídatných látek, nečistot či nečekaných složek v komplexních potravinových vzorcích. Výrobci, jako je <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy"><strong>Watrex Praha</strong></a>, nabízejí flexibilní chromatografické platformy, které pokrývají široké spektrum analytických úloh: od jednoduchých izokratických až po pokročilé gradientní a GPC/SEC aplikace, a tím podporují analytiky při zajišťování kvality potravin a ochraně spotřebitelů.</p><blockquote><p>Zaujal Vás článek? Pro více zajímavých témat sledujte <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy"><strong>Watrex Praha</strong> </a>na jejich sociálních sítích (<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/company/watrex/?viewAsMember=true"><strong>LinkedIn</strong></a> a <a target="_blank" rel="noopener noreferrer" href="https://www.facebook.com/WatrexPrague"><strong>Facebook</strong></a>).</p></blockquote>

Falšování potravin a jak ho odhalí analytická chemie?

<p>🎤 <strong>Přednášející: Michal Belianský</strong></p><h3><strong>VIZE 2025: Empower Method Validation Manager</strong></h3><h4><strong>Empower Method Validation Manager (MVM): moderní přístup k validaci chromatografických metod</strong></h4><p>Validace analytických metod je klíčovým požadavkem regulovaných laboratoří v oblasti farmacie, biotechnologií i kontroly kvality. Software <strong>Empower Method Validation Manager (MVM)</strong> od společnosti Waters představuje komplexní nástroj, který umožňuje provádět validaci chromatografických metod systematicky a plně v souladu s požadavky lékopisů a regulačních autorit.</p><p>MVM je rozšířením chromatografického datového systému <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products/627"><strong>Empower</strong></a>, díky čemuž propojuje vývoj, validaci, zpracování dat i reporting do jednoho uceleného workflow.</p><h4><strong>Automatizované výpočty a System Suitability</strong></h4><p>Jednou z hlavních výhod Empower MVM je využití <strong>automaticky generovaných chromatografických parametrů</strong>, jako jsou:</p><ul><li>rozlišení (Resolution),</li><li>faktor symetrie a tailing,</li><li>počet teoretických pater (Plate Count),</li><li>výška a šířka píku,</li><li>poměr signál/šum (S/N).</li></ul><p>Tyto parametry jsou vyhodnocovány v souladu s požadavky <strong>USP, EP i JP</strong> a mohou být přímo použity v rámci testů <strong>System Suitability</strong>, čímž se minimalizuje riziko manuálních chyb a zvyšuje konzistence výsledků.</p><h4><strong>Podpora chemických struktur a specifických aplikací</strong></h4><p>Empower MVM nabízí také rozšíření <strong>Chemical Structures</strong>, které je integrováno ve spolupráci s ACD/Labs. Díky tomu lze přímo v prostředí Empoweru:</p><ul><li>kreslit a upravovat chemické struktury,</li><li>vytvářet knihovny sloučenin,</li><li>vkládat struktury do tabulek komponent a zahrnovat je do reportů.</li></ul><p>Další specializovaná rozšíření pokrývají například:</p><ul><li><strong>Dissolution</strong> – automatizované vyhodnocení disolučních testů včetně výpočtů f1, f2 a Q faktoru,</li><li><strong>GPC/SEC pro polymerní analýzu</strong> – výpočty molekulových hmotností (Mw, Mn, Mp, Mz) a kombinaci dat z RI a LS detektorů,</li><li><strong>Sample Weight Importer</strong> – přímý přenos hmotností ze vah do Empoweru s plnou auditní stopou.</li></ul><h4><strong>Validace metod krok za krokem v MVM</strong></h4><p>Empower Method Validation Manager strukturuje validaci do tří hlavních fází:</p><ol><li><strong>Validation Protocol</strong><br>Definice validačních požadavků, akceptačních kritérií, typů testů a schvalovacích procesů.</li><li><strong>Validation Study</strong><br>Řízení celé validační studie v jednom projektu – akvizice dat, jejich zpracování, statistické výpočty a průběžné hodnocení souladu s kritérii.</li><li><strong>Reporting</strong><br>Automatizovaná tvorba validačních reportů z předdefinovaných šablon, včetně elektronických podpisů a plné sledovatelnosti změn.</li></ol><p>Software podporuje všechny klíčové validační testy, jako jsou linearita, přesnost, správnost, specificita, LOD/LOQ, opakovatelnost, mezilehlá přesnost, robustnost či stabilita metody</p><h4><strong>Integrace, migrace dat a monitoring systémů</strong></h4><p>Součástí ekosystému Empoweru jsou i nástroje pro <strong>migraci dat z jiných CDS</strong> (např. Agilent ChemStation/OpenLab, Thermo Chromeleon, MassLynx) pomocí modulu <strong>Waters Data Converter</strong>.</p><p>Díky platformě <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products/1544"><strong>waters_connect</strong></a><strong> System Monitoring</strong> mají uživatelé přehled o stovkách chromatografických a LC-MS systémů, mohou sledovat jejich stav v reálném čase, dostávat automatické notifikace a efektivně plánovat využití přístrojů, což významně zvyšuje produktivitu laboratoře.</p><h4><strong>Závěr</strong></h4><p>Empower Method Validation Manager představuje robustní a regulatorně připravené řešení pro validaci chromatografických metod od A do Z. Automatizace výpočtů, jednotné řízení validačních studií, pokročilé reportingové nástroje a hluboká integrace s CDS Empower činí z MVM silný nástroj pro laboratoře, které kladou důraz na kvalitu dat, efektivitu práce a plnou shodu s regulačními požadavky.</p>

Waters VIZE 2025: Empower Method Validation Manager

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ÚOCHB AV ČR je přední mezinárodně uznávaná vědecká instituce, jejímž hlavním posláním je základní výzkum v oblasti chemické biologie a medicinální chemie, organické a materiálové chemie, chemie přírodních látek, biochemie a molekulární biologie, fyzikální chemie, teoretické chemie a analytické chemie.

Ústav organické chemie a biochemie AV ČR

Firmu Waters založil v roce 1958 pan James Waters. Od té doby se společnost Waters specializuje na kapalinovou chromatografii a hmotnostní spektrometrii. Divize TA Instruments působí na poli termální analýzy. V České republice je Waters zastoupen přímou pobočkou, která sídlí v Praze.

Waters Gesellschaft

Watrex Praha je přední český výrobce a distributor systémů a příslušenství zejména pro kapalinovou chromatografii. Distribuujeme produkty mnoha chemických firem a máme vlastní řadu systémů za rozumnou cenu.

Watrex Praha

Separation Science

Agilent Technologies

Technology Networks

Bruker

Thermo Fisher Scientific

SCIEX

Waters Corporation

<h4><strong>Virtual event: Pharma conclave series 2024</strong></h4><p>SCIEX offers the platform for the experts to take the stage and drive technological progress</p><p><strong>Welcome to Pharma conclave series 2024</strong> – where our customers’ expertise meets innovation, transforming your knowledge and empowering you to revolutionize your work processes. Join us in discovering techniques that will enhance your skills to accelerate your daily operations in the development of new drugs.</p><p>From the research of new markers to the drug development moving into non and GLP bioanalysis. Confidently plan your next lab expansion with the knowledge gained from industry leaders and pioneers.</p><ul><li>May 16 <strong>Event 1: Pharma omics</strong></li><li>September 17 <strong>Event 2: Impurities</strong></li><li>October 23-24 <strong>Event 3: Pharma</strong></li></ul><p><strong>10:00 a.m.–12:00 p.m. CET</strong></p><h3><strong>Event 3: Pharma</strong></h3><p><strong>The pharma connection</strong></p><p>From discovery to QA/QC. All the process to bring a new treatment to the patients is following a path to connect the different stages until the magic happens.</p><p>Join to see how the scientists are generating the needed tricks to connect the initial though with the final formulation.</p><h3><strong>Day 1</strong></h3><h5><strong>10:00 - 10:30 - Metabolomics screening kits for use in clinical trials</strong></h5><ul><li>Heike Wiese, Nuvisan</li></ul><h5><strong>10:30 – 11:00&nbsp;- Sphingolipid biomarkers in neurodegenerative drug development</strong></h5><ul><li>Jose Carlos Gonzalez, AMSbiopharma</li></ul><h5><strong>11:00 – 11:30 - Parallel setup of a series of micro LC methods, coupled with the low-resolution mass spec (API7500) for &nbsp;TG2 inhibitors</strong></h5><ul><li>Davide Massari and Chiara Olmi, ERBC Group</li></ul><h5><strong>11:30 – 12:00&nbsp;- PFAS analysis and mass spectrometry: Beyond environmental, food and packaging. What about cosmetic and pharmaceutical products?</strong></h5><ul><li>Emiliano De Dominicis, Merieux</li></ul><blockquote><p><strong>Presenter:</strong> Bruno Manadas, PhD</p></blockquote><blockquote><p>Dr Manadas is a prominent Cell Biology researcher at CNC-UC since 2017. With expertise in biochemistry, psychology, and biotechnology, he's authored 130+ publications and led multiple projects. As head of CNC's Mass Spectrometry node and Associate Editor for Frontiers, he's contributed significantly to neuroscience and psychiatry. Bruno's research focuses on cell biology mechanisms, translational biomarker research, and MS-based approaches for studying protein oxidation. He played a pivotal role in establishing the hospital biobank and teaches at the Portuguese Catholic University.</p></blockquote><blockquote><p><strong>Presenter:</strong> Dorte Bekker-Jensen</p></blockquote><blockquote><p>Dorte is heading the Applications Development team in Proteomics Research at Evosep. She has long-term experience within proteomics and has focused on technology development by developing simple and automated workflows for high-throughput applications.</p></blockquote><blockquote><p><strong>Presenter:</strong> Stefan Ledinger</p></blockquote><blockquote><p>Stefan has 20 years of experience in the life sciences and diagnostic industries. For the last dozen years, he has been serving various functions within biocrates life sciences ag. Most recently, he has been responsible for the market development for metabolomics solutions in the pharmaceutical and biotech industries. He currently serves as Global Head of Sales. Stefan holds degrees in business administration and bioengineering, combining commercial know-how with a passion for science.</p></blockquote><blockquote><p><strong>Presenter:</strong> Markus Langsdorf</p></blockquote><blockquote><p>Markus has 14 years of experience in life sciences industries. As he joined biocrates in 2010, he worked for 11 years as application support specialist for biocrates’ metabolomics profiling solutions. 3 years ago he changed his position withing the company to his new function as product manager. Markus studied chemistry and graduated with a PhD in Analytical Chemistry.</p></blockquote>

Pharma conclave series 2024: Pharma (Day1)

From discovery to QA/QC. The process to bring a new treatment to the patients.

<h4><strong>Virtual event: Pharma conclave series 2024</strong></h4><p>SCIEX offers the platform for the experts to take the stage and drive technological progress</p><p><strong>Welcome to Pharma conclave series 2024</strong> – where our customers’ expertise meets innovation, transforming your knowledge and empowering you to revolutionize your work processes. Join us in discovering techniques that will enhance your skills to accelerate your daily operations in the development of new drugs.</p><p>From the research of new markers to the drug development moving into non and GLP bioanalysis. Confidently plan your next lab expansion with the knowledge gained from industry leaders and pioneers.</p><ul><li>May 16 <strong>Event 1: Pharma omics</strong></li><li>September 17 <strong>Event 2: Impurities</strong></li><li>October 23-24 <strong>Event 3: Pharma</strong></li></ul><p><strong>10:00 a.m.–12:00 p.m. CET</strong></p><h3><strong>Event 3: Pharma</strong></h3><p><strong>The pharma connection</strong></p><p>From discovery to QA/QC. All the process to bring a new treatment to the patients is following a path to connect the different stages until the magic happens.</p><p>Join to see how the scientists are generating the needed tricks to connect the initial though with the final formulation.</p><h3><strong>Day 2</strong></h3><h5><strong>10:00 - 10:30&nbsp;- Metabolomics: From target engagement to metabolic phenotyping. Applications in drug discovery</strong></h5><ul><li>Filippos Michopoulos, Astrazeneca</li></ul><h5><strong>10:30 - 11:00&nbsp;- Development of an LC-MS Method to Quantify Endogenous Urea in Mouse Plasma and BAL.</strong></h5><ul><li>Benjamin Smith, Cyprotex</li></ul><h5><strong>11:00 - 11:30&nbsp;- Optimization of extraction &amp; LC-MS conditions for the bioanalysis of antibody drug conjugates with diverse chemistries</strong></h5><ul><li>John Perkins, KCAS</li></ul><h5><strong>11:30 - 12:00&nbsp;- Preparation of rodent whole blood and plasma microsamples for LC-MS/MS bioanalysis in toxicology studies</strong></h5><ul><li>Birgitte Støvring, Scantox</li></ul><blockquote><p><strong>Presenter:</strong> Bruno Manadas, PhD</p></blockquote><blockquote><p>Dr Manadas is a prominent Cell Biology researcher at CNC-UC since 2017. With expertise in biochemistry, psychology, and biotechnology, he's authored 130+ publications and led multiple projects. As head of CNC's Mass Spectrometry node and Associate Editor for Frontiers, he's contributed significantly to neuroscience and psychiatry. Bruno's research focuses on cell biology mechanisms, translational biomarker research, and MS-based approaches for studying protein oxidation. He played a pivotal role in establishing the hospital biobank and teaches at the Portuguese Catholic University.</p></blockquote><blockquote><p><strong>Presenter:</strong> Dorte Bekker-Jensen</p></blockquote><blockquote><p>Dorte is heading the Applications Development team in Proteomics Research at Evosep. She has long-term experience within proteomics and has focused on technology development by developing simple and automated workflows for high-throughput applications.</p></blockquote><blockquote><p><strong>Presenter:</strong> Stefan Ledinger</p></blockquote><blockquote><p>Stefan has 20 years of experience in the life sciences and diagnostic industries. For the last dozen years, he has been serving various functions within biocrates life sciences ag. Most recently, he has been responsible for the market development for metabolomics solutions in the pharmaceutical and biotech industries. He currently serves as Global Head of Sales. Stefan holds degrees in business administration and bioengineering, combining commercial know-how with a passion for science.</p></blockquote><blockquote><p><strong>Presenter:</strong> Markus Langsdorf</p></blockquote><blockquote><p>Markus has 14 years of experience in life sciences industries. As he joined biocrates in 2010, he worked for 11 years as application support specialist for biocrates’ metabolomics profiling solutions. 3 years ago he changed his position withing the company to his new function as product manager. Markus studied chemistry and graduated with a PhD in Analytical Chemistry.</p></blockquote>

Pharma conclave series 2024: Pharma (Day 2)

<p><strong>We invite you to join a hybrid event on October 15<sup>th</sup> to explore the latest solutions and workflows for overcoming environmental and food contaminant testing challenges.</strong></p><p>This event will be broadcast live from the laboratory at Thermo Fisher Scientific Customer Solutions Center (CSC) in Paris. You can join in person at one of our many 'satellite' venues worldwide, where we will offer an extended agenda, or participate remotely through the live broadcast.</p><p>During the live stream broadcast, you will meet our Customer Solution Centre specialist team and leading industry expert, John Quick, as they discuss innovative solutions to meet the ever-evolving testing demands. There will be plenty of time to ask these experts your questions.</p><p>The following sessions will be streamed all over Europe and beyond:</p><ul><li><strong>Detecting PFAS in drinking water to meet regulations across Europe</strong> – New automated sample preparation high-resolution MS solution to achieve parts per trillion detection limits.<br><i>Aristide Ganci,&nbsp;Senior Product Application Specialist, Thermo Fisher Scientific; Valérie Thibert, Senior Product Application Specialist, Thermo Fisher Scientific; Bénédicte Gauriat, Senior Product Application Specialist, Thermo Fisher Scientific</i></li><li><strong>New ready-to-go pesticide residue solution for faster, more confident results</strong><br><i>Zoe Julian, Product Marketing Specialist, Manager; Bénédicte Gauriat, Senior Product Application Specialist, Thermo Fisher Scientific; Julie Moriceau, Senior Product Application Specialist, Thermo Fisher Scientific</i></li><li><strong>Low-level contaminant testing in environmental water matrices</strong>&nbsp;– High-Resolution Accurate Mass GC-MS approach to meet future regulations<br><i>John Quick, Principal Scientist, ALS Environmental Ltd</i></li></ul><p>After the live stream concludes, 'satellite' locations offer a unique program onsite. Take this unique opportunity to connect with industry professionals, or join us to explore, network, and collaborate on your future projects. Scroll below to the venue of your choice to discover the individual agenda.</p><p>Participation is free of charge, registration is required.</p><p>For any questions about this event or regarding your registration, please email <a href="mailto:CMDEvents@thermofisher.com">CMDEvents@thermofisher.com</a>.</p><h3><strong>Preliminary agenda</strong></h3><h5><strong>10:15 Live streaming of this event to ‘satellite’ locations and online audience</strong></h5><h5><strong>10:30 Introduction</strong></h5><ul><li>Jeff Stubbs (Business Development Manager, Environment and Food Safety, Thermo Fisher Scientific)</li></ul><h5><strong>10:55 Detecting PFAS in drinking water to meet regulations across Europe - New automated sample preparation high-resolution MS solution to achieve ppt detection limits</strong></h5><ul><li>A Ganci, V Thibert and B Gauriat (Senior Product Application Specialists, Thermo Fisher Scientific)</li></ul><h5><strong>11:35 Break</strong></h5><h5><strong>12:05 New ready-to-go pesticide residue solution for faster, more confident results</strong></h5><ul><li>Z Julian, Product Marketing, B Gauriat, and J Moriceau (Senior Product Application Specialists Thermo Fisher Scientific)</li></ul><h5><strong>12:45 Low-level contaminant testing in environmental water matrices – high-resolution GCMS approach to meet future regulations</strong></h5><ul><li>John Quick (Principal Scientist, ALS Environmental Ltd)</li></ul><h5><strong>13:15 Closing comments</strong></h5><h5><strong>13:30 End of live streaming</strong></h5><div><div container-id="cq-colctrl-lt7" comp-id="B1"><div><div><div><div><div><div><blockquote><h3>How to attend?</h3><p>3 ways of attending:</p><p><strong>Customer Solution Centre (in-person event):&nbsp;</strong><br>Join the heart of the event at our Customer Solution Center in Paris, where the main agenda will be hosted and live-streamed worldwide to our ‘Satellite’ locations and our audience.</p><p><strong>Join a ‘Satellite’ location (in-person event):&nbsp;</strong><br>Our in-person ‘Satellite’ events will broadcast the main agenda live-streamed from our Customer Solution Center in Paris and supplement the day with local presentations, lab tours, or workshops. Take this opportunity to meet your Thermo Fisher representatives and industry experts from the region. Discover the individual agenda for your ‘Satellite’ location of interest.</p><p><strong>Online attendees (remote):&nbsp;</strong><br>Unable to travel to a ‘Satellite’ event? Not a problem! You can register to attend conveniently online from your desk or anywhere else through the live stream from our Customer Solutions Center in Paris.</p></blockquote></div></div></div></div></div></div></div></div>

Thermo Scientific: Solutions for environmental and food contaminant testing

Solutions for environmental and food contaminant testing

New and advancing workflows to meet and exceed European regulations.

<p><strong>Join us as we introduce our path to visualize molecules from tissue at single cell resolution using DESI MS imaging, with the commercial DESI XS ionization source coupled to Xevo G3 QToF Mass Spectrometer.</strong></p><p>This webinar will explain how to easily transition to high resolution MS Imaging with a traditional entry level commercial DESI MS imaging toolkit.</p><h4>Learn how to:</h4><ul><li><strong>Understand High spatial resolution MS Imaging is now accessible to all labs and applications</strong></li><li><strong>Access single cell DESI Imaging with the combination of DESI XS and Xevo G3 QToF</strong></li><li><strong>Leverage both high sensitivity and high speed of acquisition with the Xevo G3 QToF without compromising MS Imaging data quality</strong></li><li><strong>Utilize HDI 1.8 Imaging software for microscope mode analysis for effective use of time in MS Imaging experiments</strong></li><li><strong>Obtain comprehensive and detailed spatial information with limited sample preparationAdd MSI analysis into a multi-modal imaging workflow with ease</strong></li></ul><blockquote><p><strong>Presenter:&nbsp;</strong>Dr Jamie Reilly (Postdoctoral Researcher, University of Galway)</p><p>Dr. Jamie Reilly is a postdoctoral researcher in NUIG Galway. Prior to joining the CELLIMAGE project, Jamie undertook his PhD in Regenerative Medicine in REMEDI, where he used bioinformatics to analyze molecular pathways in cellular and animal models of autism spectrum disorder (ASD). Jamie attended Trinity College Dublin (TCD) for his undergraduate degree in Genetics, and has a Masters in Biotechnology from Queens University Belfast.</p><p><strong>Presenter:&nbsp;</strong>Dr. Mark Towers (Principal Scientist - MS Technology, Waters)</p><p>Mark Towers gained his PhD from the University of Reading in Bioanalytical chemistry on the Development of applications and techniques for UV-Liquid-MALDI-MS. Mark joined Waters over 10 years ago into the Scientific operations team as a senior scientist, working on high resolution mass spectrometry and imaging.</p><p>Mark’s primary area of focus has been surface analysis by desorption electrospray ionisation (DESI) with over 7 years experience and matrix assisted laser desorption ionisation for over 14 years, principally but not limited to tissue sections for the detection and distribution mapping of a wide range of molecules.</p><p><strong>Presenter: </strong>Dr. Joanne Ballantyne (Director, MS Imaging program, Waters Corporation)</p><p>Joanne completed a PhD in Mass Spectrometry at UMIST in Manchester 2002, in collaboration with UK Ministry of Defence's Science and Technology Lab (Dstl). This was followed by a post-doc at NIH in Bethesda, USA, working in OMICS for the NIMH. After returning to the UK, and time as a MALDI applications chemist at Shimadzu Biotech, Joanne joined Waters Corporation in 2007 as a member of the OMICS Application team in Manchester and progressed to managing the team. Following this Joanne spent a number of years in Business Development and Field Marketing for discovery applications in the Northern European region. At the end of 2018 Joanne joined the Product Management group with responsibility for the SYNAPT and MS Imaging technologies. After gaining ILM7 accreditation in strategic leadership, Joanne is now Director of the MS Imaging program.</p></blockquote>

Waters Corporation: Towards Single Cell Imaging Session 1

Towards Single Cell Imaging Session 1

How to easily transition to high resolution MS Imaging with a traditional entry level commercial DESI MS imaging toolkit.

<p><strong>This webinar will guide you through optimizing your Q-TOF for superior data quality by selecting the most suitable tune mode for your samples.</strong></p><p>We will delve into various MS acquisition modes, including MS only, All Ions (QRAI), Auto MS/MS (directed MS/MS; targeted MS/MS), and PRM mode.</p><p>Learn how to fine-tune method settings to enhance your data quality and achieve the best analytical results. Whether you’re a seasoned user or new to QTOF, this session will provide valuable insights to elevate your mass spectrometry.</p><blockquote><p><strong>Presenter:</strong> &nbsp;Stefan Schaebler (Application Specialist LC/MS, Agilent Technologies)</p><p>Stefan completed his PhD in Biology, specializing in LC-MS-based lipidomics and metabolomics. Following his doctoral studies, he joined Agilent Technologies as an LC/MS Application Specialist. In this role, Stefan focuses on small molecule applications using LC-QTOF, with a strong emphasis on software tools and data interpretation.</p></blockquote>

Agilent Technologies: MassHunter Masterclass Months

MassHunter Acquisition Q-TOF Part II – Advance Settings

Join us for an in-depth exploration of advanced settings in MassHunter Acquisition for Q-TOF systems.

<div><div><p><strong>Therapeutic synthetic oligonucleotides represent unique analytical challenges in late-stage development. To meet the stringent regulatory requirements, highly specific and sensitive methods such as liquid chromatography-mass spectrometry (LC/MS) must be used. However, neither chromatography nor mass spectrometry data systems have the capabilities, both in function and in data integrity, for the typical QC analyst end user to run a highly complex method such as purity, assay, and impurity profiling for therapeutic oligos such as anti-sense oligonucleotides.</strong></p><p>In this presentation, we present a paradigm shift in software with Oligo Analysis Accelerator for OpenLab CDS. This application streamlines the analytical workflow by using a fit-for-purpose user interface while leveraging existing data integrity and compliance architecture within OpenLab CDS.</p><h4>By attending this webinar, you will:</h4><ul><li><strong>Understand the fundamentals of ion-pair reversed-phase LC/MS for oligonucleotides</strong></li><li><strong>Discover how a single quadrupole detector is used for impurity profiling of anti-sense oligonucleotides</strong></li><li><strong>Learn how fit-for-purpose software for LC/MS analysis in QC lot release can save time and enhance quality</strong></li></ul><h5>Who should attend</h5><ul><li>Analytical chemists interested in cutting-edge software for large molecule late-stage development</li><li>Analytical research and development labs interested in developing robust methods for single-stranded oligo therapeutics</li><li>Process scientists who rely on in-process control purity results to optimize synthesis and purification for oligos</li><li>Quality assurance and quality control personnel supporting oligo testing labs</li></ul><blockquote><p><strong>Presenter:</strong> Brian Rivera (Software Product Manager, Agilent Technologies)</p><p>Brian Rivera is a software product manager at Agilent Technologies with 20 years of experience in HPLC analysis of large molecules.</p></blockquote></div></div>

Separation Sciences: Boost Efficiency in Oligonucleotide QC with Software Designed for the Job

Boost Efficiency in Oligonucleotide QC with Software Designed for the Job

In this presentation, we present a paradigm shift in software with Oligo Analysis Accelerator for OpenLab CDS.

Oligonucleotide QC with Software Designed for the Job

<p id="isPasted"><strong>Biotherapeutics offer targeted therapies for treating a wide range of diseases with greater specificity and efficacy. However, unlike traditional small-molecule drugs, biotherapeutics are typically larger and more complex, requiring advanced techniques for their production, purification, and characterization.</strong></p><p>Therapeutic proteins, first introduced many years ago, have continually advanced in complexity. Since the introduction of monoclonal antibodies, we now have antibody drug conjugates, bispecific antibodies, fragments and fusion proteins, all of which are in various stages of development.&nbsp;</p><p>To keep pace with biotherapeutics advances, analytical techniques must also advance.&nbsp;</p><h5><strong>In this webinar, we will present chromatography findings on:</strong></h5><ul><li><strong>New workflows for intact and peptide mapping characterization of proteins</strong></li><li><strong>Advanced column chemistries for charge variant analysis with pH and salt gradients&nbsp;</strong></li><li><strong>Rapid desalting SEC column for high throughput analysis, ADC analysis with HIC and reversed phase chemistries</strong></li><li><strong>New digestion workflows coupled to a new peptide mapping column for sequence verification and post translation modification analysis</strong></li></ul><p>This live webinar will be an excellent opportunity to boost your R&amp;D capabilities and strengthen your QC analytical methods for the characterization of biotherapeutics and gene therapies. Dr. Ken Cook will be presenting and answering your questions live.</p><p>With over 20 years of innovation, Thermo Fisher Scientific is a leader in biomolecule separation and characterization, supported by a wealth of published research.</p><p id="isPasted"><strong fr-original-style="">Who should attend?&nbsp;</strong></p><ul><li><strong fr-original-style="">Pharmaceutical and Biotech Researchers</strong>: Researchers involved in the development and characterization of biotherapeutics and gene therapies, including those working with therapeutic proteins.</li><li><strong fr-original-style="">Quality Control (QC) Analysts</strong>: Analysts responsible for ensuring the quality and consistency of biotherapeutic products, who will benefit from advanced methods in characterization and validation.</li><li>&nbsp;<strong fr-original-style="">R&amp;D Managers</strong>: Managers who oversee research and development teams working with biotherapeutics, looking to enhance their workflows and methodologies.</li></ul><blockquote><p><strong>Presenter:</strong> Dr. Ken Cook (Application Scientists Manager, Thermo Fisher Scientific)</p><p>Dr. Ken Cook began his career in academia as a Biochemistry lecturer focused on protein chemistry and metabolism. With over 30 years of industry experience at Dionex, and later Thermo Fisher Scientific, his vast knowledge supporting and developing solutions within chromatography has established him as a bio-separations expert.</p><p>Currently, Dr. Cook focuses on supporting and developing solutions for bio-pharmaceutical applications, particularly in the characterization of protein and oligonucleotide-based biotherapeutics. His work involves ongoing collaborations with both the biopharmaceutical industry and academic institutions.</p><p>His expertise includes peptide mapping using LC/HRMS, charged variant analysis with novel MS coupling, glycan analysis, aggregate analysis, and on-line process monitoring. He also has a growing interest and notable success in gene therapy characterization.</p><p>Given his immense experience, it is an honour to have Dr Cook share his knowledge.</p></blockquote>

Thermo Fisher Scientific: Innovations in Protein Characterization

Innovations in Protein Characterization

Boost your R&D capabilities and strengthen your QC analytical methods for the characterization of biotherapeutics and gene therapies.

<div><div><div><p><strong>Alithea Biotechnology was founded in 2019 by Fanny Giannou and Tim Fugmann to support Biopharma globally on the development of innovative therapies targeting HLA-presented peptides. Alithea has a significant track record in the absolute quantification of HLA peptides, off-target toxicity predictions and target discovery.</strong></p><p>In addition, HLA-Compass is Alithea's cloud-accessible HLA peptide database featuring the measurement of a total of 1.4 Million HLA peptides in 4160 healthy tissue, tumor tissue and cancer cell line samples for target validation and off-target toxicity prediction.</p><h5><strong>Why to join this webinar:</strong></h5><ul><li><strong>The timsTOF Ultra can lead to high quality immunopeptidomes from limited sample</strong></li><li><strong>Extensive HLA databases can significantly speed up the T-Cell-Receptor therapy development process</strong></li><li><strong>The time is ripe to bring immunopeptidomics into clinical routine</strong></li></ul><blockquote><p><strong>Presenter:</strong> Tim Fugmann, Ph.D. (Chief Scientific Officer and Co-Founder, Alithea Biotechnology GmbH, Berlin, Germany)</p><p>Tim Fugmann got his Ph.D. from ETH Zurich, on mass-spectrometric discovery of antibody targets and disease biomarkers. Later, he was leading the target discovery efforts at Philochem, before moving to the Max-Delbrueck-Centrum for Molecular Medicine in Berlin. He was later appointed professor for MS-based proteomics at JLU Giessen. In 2019 he co-founded Alithea Bio UG, which is focusing on immunopeptidomics, a topic which Tim Fugmann studied since 2012.</p></blockquote></div></div></div>

Bruker: Unlocking the full potential of immunopeptidomics with the timsTOF Ultra

Unlocking the full potential of immunopeptidomics with the timsTOF Ultra

Alithea has a significant track record in the absolute quantification of HLA peptides, off-target toxicity predictions and target discovery.

The potential of immunopeptidomics with the timsTOF Ultra

<p><strong>Hear about the latest advancements in translational proteomics and metabolomics. From the molecular mechanisms of disease pathology to the latest technological developments in diagnostics and treatment.</strong></p><h5><strong>Key Learning Objectives:</strong></h5><ul><li>Spatial proteomics to assess health and diseases</li><li>Absolute quantitation of human proteins</li><li>The latest innovations in proteomics and metabolomics instrumentation and data analysis</li><li>Biomarker-guided precision medicine</li></ul><h4><strong>Day 1 – October 30</strong></h4><h5><strong>15:00 – 15:50 GMT: The Human Protein Atlas – Creating a Pan-Disease Resource Based on Next Generation Blood Profiling</strong></h5><ul><li>Mathias Uhlén, PhD (KTH Royal Institute of Technology)</li></ul><h5><strong>15:50 – 15:55 GMT:</strong> Networking Break</h5><h5><strong>15:55 – 16:15GMT: Mitigating Sorter-Induced Cellular Stress with Cartridge, Microfuildics-Based Sorting</strong></h5><ul><li>Crystal Montoya (Miltenyi Biotec)</li></ul><h5><strong>16:15 – 16:20 GMT:</strong> Networking Break</h5><h5><strong>16:20 – 16:40 GMT: Speaker to be announced</strong></h5><h5><strong>16:40 – 16:45 GMT</strong>: Networking Break</h5><h5><strong>16:45 – 17:35 GMT: Spatial Tissue Proteomics to Assess Health and Disease</strong></h5><ul><li>Fabian Coscia, PhD (Max Delbrück Center)</li></ul><h5><strong>17:35 – 17:40 GMT:</strong> Networking Break</h5><h5><strong>17:40 – 18:00 GMT: The Sulpho-Phospho-Vanillin Assay: Accurate Lipid Normalization in Quantitative Lipidomics</strong></h5><ul><li>Karri Basso, PhD (University of Florida)</li></ul><h5><strong>18:00 – 18:05 GMT:</strong> Networking Break</h5><h5><strong>18:05 – 18:55 GMT: Proteomics for Cancer Precision Medicine</strong></h5><ul><li>Janne Lehtiö, PhD (Karolinska Instutet)</li></ul><h5><strong>18:55 – 19:00 GMT: </strong>Networking Break</h5><h5><strong>19:00 – 19:20 GMT: How Breakthroughs in Depth, Scale and Speed are Unlocking New Proteomic Discovery</strong></h5><ul><li>Aaron Gajadhar, PhD (Seer Bio)</li></ul><h5><strong>19:20 – 19:25 GMT: </strong>Networking Break</h5><h5><strong>19:25 – 19:55 GMT:</strong> Phantom Metabolites: Unraveling Metabolic Shadows</h5><ul><li>Gary Siuzdak, PhD (Scripps Research Institute)</li></ul><h5><strong>19:55 GMT:</strong> End of Day 1</h5><div><div><div><h5><strong>Who Should Attend:</strong></h5><ul><li>Scientists and researchers seeking to expand their knowledge</li><li>Pharmaceutical and biotech professionals driving drug development</li><li>Clinicians looking to improve patient care</li><li>Academicians exploring new research avenues</li><li>Graduate students and postdoctoral fellows building their careers</li></ul><blockquote><p><strong>Presenter: </strong>Mathias Uhlén, PhD (Professor, KTH - Royal Institute of Technology)</p><p><strong>Presenter:&nbsp;</strong>Christoph Borchers, PhD (Professor, McGill University)</p><p><strong>Presenter:</strong>&nbsp;Janne Lehtiö, PhD (Professor, Karolinska Institutet)</p><p><strong>Presenter:</strong>&nbsp;Crystal Montoya (Senior Marketing Product Manager, Miltenyi Biotec)</p><p><strong>Presenter:</strong>&nbsp;Kari Basso, PhD (Director of Mass Spectrometry Research and Education, University of Florida)</p><p><strong>Presenter:&nbsp;</strong>Fabian Coscia, PhD (Group Leader, Max Delbrück Center)</p><p><strong>Presenter:</strong>&nbsp;Simone Sidoli, PhD (Assistant Professor, Albert Einstein College of Medicine)</p><p><strong>Presenter:</strong>&nbsp;Hagen Magnus Gegner, PhD (Content and Digital Marketing, CTC Analytics)</p><p><strong>Presenter:</strong>&nbsp;Matt Willetts, PhD (Proteomics Applications Manager, Bruker Daltonics)</p><p><strong>Presenter:&nbsp;</strong>Matteo Tardelli, PhD (Senior Biomarker Scientist, Owlstone Medical)</p><p><strong>Presenter:&nbsp;</strong>Aaron Gajadhar, PhD (Director, Strategic Applications, Seer)</p><p><strong>Presenter:&nbsp;</strong>Gary Siuzdak, PhD (Professor and Senior Director, Scripps Research Institute)</p></blockquote></div></div></div>

Technology Networks: Advances in Proteomics & Metabolomics 2024

Advances in Proteomics & Metabolomics 2024 (Day 1)

Hear about the latest advancements in translational proteomics and metabolomics. 

Advances in Proteomics & Metabolomics 2024  (Day 1)

<div><div><div><p><strong>Pure MHC, a pioneer in immunopeptidomics, has partnered with Bruker, a leader in state-of-the-art mass spectrometry, to demonstrate one of Pure MHC’s immunopeptidomics workflows. In this Live from the Lab webinar, tissue sample processing, data acquisition with a Bruker timsTOF Ultra, and data analysis will be reviewed as tools for the development and validation of HLA peptide targets for immunopeptidomic development.</strong></p><p><strong>Key Learning Objectives:</strong></p><ul><li>Get insights into Pure MHC's sample preparation strategies to maximize the yield of immunopeptides from tissue samples</li><li>Discover how the unparalleled sensitivity and robustness of the Bruker timsTOF Ultra empowers users to maximize the potential of their immunopeptide samples</li><li>Understand data processing workflows for both DDA and DIA data processing</li></ul><blockquote><p><strong>Presenter:</strong> Curtis McMurtrey, Ph.D. (Director of Immunoproteomics, Pure MHC, LLC, Oklahoma City, OK, USA)</p><p>Dr. McMurtrey earned his Ph.D. in Microbiology and Immunology from the University of Oklahoma Health Sciences Center in 2010. He currently serves as the Director of Immuno-Proteomics at Pure MHC, LLC.</p><p>Dr. McMurtrey has studied HLA and its role in immunity since 2005. He is a member of American Society for Histocompatibility and Immunogenetics (ASHI) and holds the distinction of having won four ASHI Top Scholar Awards, more than any other scientist in the history of ASHI. Dr. McMurtrey is most notable for his work on West Nile virus epitope discovery and validation with HLA-A*02:01 and B*07:02; epitopes he discovered have been successfully tested in a WNV vaccine (collaboration with Drs. Hansen and Diamond at Washington University). His most recent accolades come from discovery of MR1 ligands from Mycobacterium tuberculosis (Mtb) as well as over 200 novel ligands from Toxoplasma gondii utilizing Deep Ligand Sequencing (DLS).</p><p>Dr. McMurtrey has expertise in BSL-3 containment techniques, protein purification, HLA ligand isolation, HPLC separation, mass spectrometric analysis, ELISPOT, and multicolor flow cytometry as well as provides bioinformatics expertise. To date, he has directed mass spectrometry (both IDA and SWATH) acquisition as well as comparative analysis and interpretation for a wide variety of MHC ligands including those from tuberculosis, toxoplasmosis, influenza, WNV, HIV and multiple cancers. Dr. Curtis McMurtrey oversees DLS acquisition and analyses and also manages database library collection.</p><p><strong>Presenter: </strong>Jason R. Krawic, Ph.D. (Business Development Manager, Pure MHC, LLC, Oklahoma City, OK, USA)</p><p>Dr. Krawic earned his Master’s degree in Biomolecular Sciences from Central Connecticut State University in 2012 before returning to academia and earning his Ph.D. in Microbiology and Immunology from the University of Oklahoma Health Sciences Center in 2024. He currently serves as the Director of Business Development at Pure MHC, LLC.</p><p>Dr. Krawic began his scientific career teaching Microbiology, Biology, and Chemistry to community college students in Connecticut and Oklahoma. He has studied HLA and non-classical MHC molecules since 2018. He is most recognized for discovering multiple isomeric conformations of MR1 ligands, as well as his work in MHC Class I and Class II ligands and cancer immunotherapies.</p><p>Dr. Krawic’s expertise ranges from classical microbiology and bacteriology to HPLC separation, protein purification, negative ion mode small molecule mass spectrometry, DDA, DIA and MRM acquisition and a variety of bioinformatics tools. In addition to his scientific career, Dr. Krawic has more than a decade of experience in sales and retail management. With this skill set he combines his science and sales backgrounds to serve Pure MHC’s customer base.</p><p><strong>Presenter: </strong>Kris Looney, MBA (President, Pure MHC, LLC, Austin, TX, USA)</p><p>As President of Emergent Technologies, the manager of Pure MHC, Kris Looney is responsible for conceiving and executing key business initiatives, developing strategic plans, and managing business processes including accounting, marketing, intellectual property, and business development. Kris has more than two decades of experience as a business generalist with a background in start-ups, management, accounting, marketing, negotiation, and sales.</p><p>Before joining Emergent, Kris served as the COO and Interim CEO of the American Red Cross of Central Texas, where he balanced multiple stakeholder needs with service responsibilities in a competitive philanthropic environment. Prior to that, he was the CFO of Transformation Enzyme Corporation, where his financial management and business development helped a bootstrapped entrepreneurial start-up become a leader in the nutritional supplement market. Kris has a B.A. in marketing and an M.B.A. from the University of Texas at Austin.</p><p><strong>Presenter: </strong>Matt Willetts, Ph.D. (Proteomics Applications Manager, Bruker Scientific LLC, Billerica, MA, USA)</p><p>Matt received his Ph.D. in mass spectrometry with Prof. Malcolm Clench at Sheffield Hallam University in the UK. He then joined Waters as the Technical Marketing Specialist for LCMS and MALDI products, focusing on application development for proteomics-based workflows. Matt joined Bruker in 2010 and is responsible for proteomics applications and support in North America.</p></blockquote><p>&nbsp;</p></div></div></div>

Bruker: Pure MHC's immunopeptidomics workflow with the timsTOF Ultra

Pure MHC's immunopeptidomics workflow with the timsTOF Ultra

Tissue sample processing, data acquisition with a Bruker timsTOF Ultra, and data analysis for immunopeptidomic development.

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