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Evaluation of search engines for phosphopeptide identification and quantitation

 

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Sensitive and Accurate Quantitation of Phosphopeptides Using TMT Isobaric Labeling Technique
Xiaoyue Jiang1, Ryan Bomgarden2, Joseph Brown1, Rosa Viner1, Andreas R Huhmer1 1 Thermo Fisher Scientific, San Jose, CA; 2Thermo Fisher Scientific, Rockford, IL Poster Note 64779 Sensitive and Accurate Quantitation of Phosphopeptides Using TMT Isobaric Labeling Technique Sensitive and accurate…
Klíčová slova
phosphopeptides, phosphopeptidesphosphopeptide, phosphopeptidetrigger, triggerconfident, confidenthcd, hcdpeptides, peptidestmt, tmtneutral, neutrallocalizaton, localizatonunenriched, unenrichedloss, losssps, spsphosphoproteome, phosphoproteomemsa, msapeptide
Optimized Search Strategy to Maximize PTM Characterization and Protein Coverage in Proteome Discoverer Software
Xiaoyue Jiang1, David Horn1, Michael Blank1, Devin Drew1, Bernard Delanghe2, Rosa Viner1, Andreas FR Huhmer1 1 Thermo Fisher Scientific, San Jose, CA, USA; 2Thermo Fisher Scientific, Bremen, Germany Key Words Protein coverage, post-translational modification (PTM), Proteome Discoverer, SEQUEST, Byonic, Mascot,…
Klíčová slova
byonic, byonicsequest, sequestsearch, searchmaxquant, maxquantpropionyl, propionylmascot, mascotpeptide, peptidefdr, fdrprotein, proteinproteome, proteomeengines, enginesdiscoverer, discovererengine, engineptm, ptmhela
Thermo Scientific Proteome Discoverer software
Thermo Scientific Proteome Discoverer software
2022|Thermo Fisher Scientific|Brožury a specifikace
The intelligent protein informatics platform Thermo Scientific Proteome Discoverer software Transform proteomics mass spectrometry data into insights Thermo Scientific™ Proteome Discoverer™ software enables comprehensive proteomics data processing workflows empowered by artificial intelligence. • Powerful and flexible framework: Optimized analysis for…
Klíčová slova
inferys, inferysdiscoverer, discovererrescoring, rescoringproteome, proteomepsms, psmschimerys, chimerysworkflows, workflowslfq, lfqpeptides, peptidessearch, searchtmt, tmtpeptide, peptidesequest, sequestproteomics, proteomicsconsensus
IMSC: Optimization of crosslinked peptide analysis on an Orbitrap Fusion Lumos mass spectrometer
Thermo Scientific™ Proteome Discoverer™ using a XlinkX software node. Methods: Different amine-reactive, homobifuctional crosslinkers including disuccinimidyl Results: For both DSSO and BuUrBu, we identified over 40 BSA inter-crosslinked peptides 2 CID for DSSO. We also to less than(DSS), 20 using…
Klíčová slova
crosslinked, crosslinkedbuurbu, buurbucleavable, cleavablecrosslinkers, crosslinkersethcd, ethcddsso, dssoprotein, proteinpeptides, peptidescrosslinking, crosslinkingidentified, identifiedxlinkx, xlinkxsequestht, sequesthtcid, cidlumos, lumosbsa
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LabRulez s.r.o. Všechna práva vyhrazena. Obsah dostupný pod licencí CC BY-SA 4.0 Uveďte původ-Zachovejte licenci.