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High-throughput plasma proteomics: A standardized and scalable workflow for quantitative protein profiling in large sample cohorts

Aplikace | 2019 | Thermo Fisher ScientificInstrumentace
LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap
Zaměření
Proteomika
Výrobce
Thermo Fisher Scientific
Klíčová slova
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APPLICATION NOTE 65952 A robust mass spectrometer for precision medicine – the Orbitrap Exploris 240 mass spectrometer for large-scale plasma protein profiling Authors: Michelle L Dubuke1, Sitara Chauhan1, Sarah Trusiak1, and Emily I. Chen1 1 Thermo Fisher Scientific Precision Medicine…
Klíčová slova
evosep, evosepprotein, proteinplasma, plasmagroups, groupsprofiling, profilingworkflow, workflowproteins, proteinsorbitrap, orbitrapswissprot, swissprotserum, serumfasta, fastahuman, humansolution, solutionweeks, weeksthermo
An Ultra High-throughput Plasma Protein Profiling (uHTPPP) Workflow Using a Modified Quadrupole-Orbitrap Mass Spectrometer Michelle L. Dubuke1, Sarah Trusiak1, Sitara Chauhan1, Ryan D. Bomgarden2, Sergei Snovida2, Bhavin Patel2, Emily I. Chen1. 1Thermo Fisher Scientific, Precision Medicine Science Center, Cambridge, MA.…
Klíčová slova
serum, serumgroups, groupsdepleted, depletedhamilton, hamiltonprotein, proteinspin, spinpmsc, pmscproteins, proteinsidentifications, identificationspeptide, peptideplasma, plasmaquantified, quantifiedhela, helaplate, platerecovery
APPLICATION NOTE 65727 Sampling the human population – ultra-highthroughput plasma protein profiling (uHTPPP) sample preparation for translational proteomics Authors: Sarah Trusiak,1 Sergei Snovida,2 Bhavin Patel,2 Ryan Bomgarden,2 Emily I. Chen,1 1 Thermo Fisher Scientific Precision Medicine Science Center, Cambridge, MA,…
Klíčová slova
uhtppp, uhtppppeptide, peptideserum, serumhamilton, hamiltoneasypep, easypepworkflow, workflowplate, platerecovery, recoveryscript, scriptspin, spinthermo, thermoplasma, plasmapipetting, pipettingsample, samplelysis
Proof of performance Orbitrap Exploris 240 mass spectrometer Plasma profiling–reliable large cohort analysis with a fully automated workflow Summary Here we present an Ultra-High Throughout Plasma Protein Profiling (uHTPPP) workflow (Figure 1), from automated sample preparation to high-resolution, accurate-mass (HRAM)…
Klíčová slova
evosep, evosepexploris, explorisorbitrap, orbitrapone, oneuhtppp, uhtpppworkflow, workflowpeptide, peptidecohort, cohortsample, sampleautomated, automatedprotien, protienstarlet, starletevotip, evotipprep, prepdipvpkpk
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