XBridge Glycan BEH Amide, 130Å, 2.5 μm XP and 3.5 μm Columns and Standards

[ CARE AND USE MANUAL ]

1

CONTENTS

I.  

INTRODUCTION

II. 

GETTING STARTED 

a.  Column Installation 

b.  Column Equilibration 

c.  Initial Column Efficiency Determination 

d.  Conditioning of Previously 

  Unused Columns 

e.  Useful Functional Tests for  

  Benchmarking a New Column 

f.  Scalable UPLC and HPLC BEH Glycan  

  Column Offerings

III. 

COLUMN USE 

a.  Sample Preparation 

b.  Operating pH Limit 

c. Solvents 

d. Pressure 

e. Temperature

IV. TROUBLESHOOTING

V. 

COLUMN CLEANING, REGENERATING,  

AND STORAGE 

a.  Cleaning and Regeneration 

b. Storage

VI. 

CAUTIONARY NOTE

VII. 

ORDERING INFORMATION

I. INTRODUCTION
Thank you for choosing a Waters® XBridge® Glycan BEH Amide, 
130Å Guard or Column containing our 2.5 µm XP or 3.5 µm 
particles designed for HILIC separations of 2-aminobenzamide 
(2-AB), 2-aminobenzoic acid (2-AA), or Waters RapiFluor-MS™ 
(p/n: 176003635) labeled glycans. This column chemistry, 
when used on an appropriately configured LC instrument, 
is capable of separating both neutral and charged labeled 
glycan species. Retention of 2-AB, 2-AA, or RapiFluor-MS 
labeled oligosaccharides is based on the hydrophobicity of the 
molecule, a parameter that is broadly related to hydrodynamic 
volume or molecular size. The resolving power of these 
columns is due in part to the particle size of the fully porous 
packing materials. Chemical and mechanical stability of the 
column are the consequence of Waters ethylene bridged hybrid 
(BEH Technology™) particle composition.

The column may be calibrated using one of the labeled Waters 
Dextran Calibration Ladder Standards (p/n: 186006841), 
such that elution may be expressed in terms of glucose units 
(GU). Under the suggested chromatographic conditions, the 
retention of Waters RapiFluor-MS, 2-AB, or 2-AA  labeled 
oligosaccharides on the XBridge Glycan BEH Amide 2.5 µm XP 
or 3.5 µm Column may be predicted based on the hydrophilic 
contributions of the individual constituent monosaccharides.

XBridge Glycan BEH Amide, 130Å, 2.5 µm XP 

and 3.5 µm Columns and Standards

2

XBridge Glycan BEH Amide, 130Å, 2.5 µm XP and 3.5 µm Columns and Standards

[ CARE AND USE MANUAL ]

II. GETTING STARTED
Each XBridge Glycan BEH Amide Column has a Certificate 
of Analysis and a Performance Test Chromatogram. The 
Certificate of Analysis is specific to each batch of packing 
material and includes the batch number and analyses of the 
physical and chemical properties of the particle. Particle 
size and pore structure are analyzed prior to bonding. The 
carbon and nitrogen content of the bonding are measured 
to insure consistent coverage. The selectivity of each batch 
is also assessed with the chromatographic separation of 
Waters Glycan Performance Standard (p/n: 186006349), a 
2-AB labeled N-linked glycan standard mix spiked with Man-5 
and Man-6, derived from human IgG. The complex mixture 
of IgG glycans includes high mannose structures as well as 
neutral and acidic complex structures. The retention times and 
retention time differences of selected components are used as 
the quality control test for each batch of packing material. The 
Performance Test Chromatogram is specific to each individual 
column and contains the following information: batch number, 
column serial number, backpressure, USP plate count, reduced 
plate height (RPH), USP tailing factor, retention factor (k�), 
peak width, and chromatographic conditions. These data can 
be found in the documentation supplied for each column and 
should be stored for future reference.

a. Column Installation

Note: The flow rates given in the procedure below are for a typical 2.5 µm XP 

packing in a 2.1 mm I.D. column. It is also highly recommended that a column 

heater be used with our XBridge Glycan BEH Amide Column to help ensure 

consistent and reproducible separated component retention times.
1.  Purge the solvent delivery system of any buffer-containing 

or water-immiscible mobile phases and connect the inlet 
end of the column to the injector outlet. An arrow on the 
column identification label indicates the correct direction 
of solvent flow.

2.  Flush the column with 100% organic mobile phase 

(acetonitrile) by setting the pump flow to 0.1 mL/min 
and increase the flow to 0.17 mL/min over 3 minutes. 
Increase the aqueous phase to 90% over 15 minutes. Note 
the backpressure. Decrease aqueous phase to starting 
conditions (22% aqueous in the test chromatogram).

3.  When the mobile phase is flowing freely from the column 

outlet, stop the flow and attach the column outlet to the 
detector. This prevents entry of air into the detection system 
and gives more rapid baseline equilibration.

4.  Gradually increase the flow rate from 0.17 to 0.34 mL/min 

over 3 minutes.

5.  Once a stable backpressure and baseline have been 

achieved, proceed to the next section

b. Column Equilibration 
Glycan Separation Technology Columns are shipped in 
100% acetonitrile. It is important to ensure mobile phase 
compatibility before changing to a different mobile-phase 
system. Equilibrate the column with a minimum of 10 column 
volumes of the mobile phase to be used (refer to Table 1 for 
column volumes).

Table 1. Empty Column Volumes in mL 
(multiply by 10 for flush solvent volumes)

Column Length 

(mm)

Column Internal 

Diameter (mm)

Empty Column 

Volume (mL)

50

2.1

0.17

100

2.1

0.35

150

2.1

0.52

30

3.0

0.21

75

3.0

0.53

150

3.0

1.06

50

4.6

0.83

100

4.6

1.66

150

4.6

2.49

250

4.6

4.15

To avoid precipitating mobile-phase buffers on your column 
or in your system, flush the column with five column volumes 
of a water/organic solvent mixture using the same or higher 
acetonitrile content as in the desired buffered mobile phase. 
For example, flush the column and LC system with 50% 
acetonitrile in water prior to introducing 50% acetonitrile/ 
50% buffered mobile phase.

The grade and quality of solvents are important for glycan 
analysis.  It is strongly recommended to use only LC-MS grade 
reagents for glycan analysis mobile phase eluents. Use of 
Waters ammonium formate solution (p/n: 186007081) is also 
highly recommended in preparing Eluent A. It is a concentrate 
made from LC-MS grade reagents and will reduce the 
generation of undersired labeled glycan adducts.

Column equilibration may be judged initially by stable 
pressure and by a stable detector baseline. For a specific 
application, it is, however, necessary to test the required 
duration of equilibration. The criteria for adequate 
equilibration include reproducibility of retention time for 
major and minor peaks, resolution for critical pairs, and 
consistent baseline characteristics.
Note: Low concentration mobile-phase additives, particularly those 

with minimal buffering capacity, may require extended equilibration 

and re-equilibration between gradient analyses.

3

XBridge Glycan BEH Amide, 130Å, 2.5 µm XP and 3.5 µm Columns and Standards

[ CARE AND USE MANUAL ]

c. Initial Column Efficiency Determination 
1.  Perform an efficiency test on the column before using 

it in the desired application. Waters recommends using 
the solute mixture and conditions described in the 
“Performance Test Chromatogram” to test the column 
upon receipt.

2.  Measure the retention of the test compounds and the 

number of theoretical plates (N). 

3.  Repeat the test at predetermined intervals to track column 

performance over time. Slight variations may be obtained 
on two different LC systems due to the quality of the 
connections, operating environment, system electronics, 
reagent quality, condition of column, and operator technique.

d. Conditioning of Previously Unused Columns
It is a good practice to ensure that new (previously unused) 
Glycan BEH Amide, 130Å columns are well conditioned and 
providing optimal performance before actual test sample 
analyses. This can be accomplished via sequential injections 
of a representative sample until a stable chromatographic 
profile is achieved. 

It should also be noted that even if a Glycan BEH Amide column 
is to be used with an ammonium formate mobile phase, it can 
still prove useful to first condition it with a gradient and mobile 
phases containing 0.1% TFA. TFA can be effective in cleaning a 
column’s stationary phase by both neutralizing and ion pairing 
contaminants that in their ionic form might otherwise strongly 
adsorb to a HILIC stationary phase. 

e. Useful Functional Tests for Benchmarking 
a New Column
We suggest use of Waters 2-AB labeled Glycan Performance 
Standard (p/n: 186006349) to benchmark your new column 
and monitor its performance during use. Note: This same 2-AB 
Glycan Performance Standard is used by Waters manufacturing 
to quality control test each batch of XBridge Glycan BEH Amide 
130Å 2.5 µm XP and 3.5 µm material designed for this application 
(See example in Figure 1).

To prepare the standard, add 100 µL of 50 mM ammonium 
formate buffer pH 4.4 and 100 µL of acetonitrile directly to 
the vial for a total volume of 200 µL.

Gently mix the sample by inversion.

Glycan Performance Test Standard
The separation shown in Figure 1 was generated on an 
ACQUITY® UPLC® whose total system volume and post 
column dispersion characteristics make it ideally suited 
for this application. Note that similar results can be 
obtained on an appropriately configured HPLC system. It 
is also important to take note of the injection solvent and 
injection volume for this application. Larger glycans have 
limited solubility in solutions that contain more than 50% 
acetonitrile. Gradual precipitation and loss of these larger 
glycans will be observed under these condtions. However, 
also note that large injections of water-containing samples 
will distort the peak shape in HILIC chromatography. 
Consequently, the optimum injection volume for these 
applications is <3 µL.

4

XBridge Glycan BEH Amide, 130Å, 2.5 µm XP and 3.5 µm Columns and Standards

[ CARE AND USE MANUAL ]

Figure 1. Typical chromatogram of 2-AB labeled human IgG N-linked glycans 

using the Glycan Performance Test Standard (p/n: 186006349).

Note: Use of the scaled gradient shown below on an XBridge Glycan BEH 

Amide, 3.5 µm 130Å, 2.1 x 150 mm Column (p/n: 186007504) should yield 

results similar to Figure 1 that was generated on a XBridge Glycan BEH Amide, 

130Å, 2.5 µm, XP, 2.1 x 150 mm Column (p/n: 186007265).

LC CONDITIONS
LC system:  

ACQUITY UPLC H-Class Bio 

Detection:  

ACQUITY UPLC FLR Detector/ 

2475 FLR Detector 

Excitation: 

330 nm 

Emission: 

420 nm 

Scan rate: 

10 Hz 

Time const.: 

0.2 sec   

Gain: 1.00 
Column:  

XBridge Glycan BEH Amide, 130Å, 

2.5 µm XP, 2.1 x 150 mm (p/n: 186007265) 

Column temp.:   60 °C 
Sample temp.:   15 °C 
Mobile phase A:  100 mM ammonium formate, 
 

pH 4.5 

Mobile phase B:  Acetonitrile (ACN) 
Vials:  

LCGC Certified Clear Glass 12 x 32 mm  

Screw Neck Qsert Vial (p/n: 186001126C) 

Gradient: 

Time 

Flow Rate            Profile 

(min) 

(mL/min) 

%A 

%B 

0.00  0.34 

22.00 78.00 

56.62 0.34 

44.10 55.90 

58.09  0.1 

80.00 20.007 

65.44  0.17 

80.00 20.00 

68.38 0.34 

22.00 78.00 

75.53  0.34 

22.00 78.00

Column:  

XBridge Glycan BEH Amide, 130Å, 3.5 µm,   

2.1 x 150 mm (p/n: 186007504) 

Gradient: 

Time 

Flow Rate              Profile 

(min) 

(mL/min) 

  %A 

%B 

0.00  0.24 

22.00 78.00 

79.26 0.20 

44.10 55.904 

81.32  0.12 

80.00 20.00 

91.62  0.12 

80.00 20.00 

95.74  0.24 

22.00 78.00 

102.94 0.24 

22.00  78.00

Peak Identification

1. G0 (NGA2)

8. G1F+GN

2. G0F (NGA2F)

9. Man-6

3. Man-5

10. G2 (NA2)

4. G0F+GN

11. G2F (NA2F)

5. G1

12. G1F+SA

6. G1Fa (NA2G1F)

13. G2F+SA (A1F)

7. G1Fb (NA2G1F) 

10

12

14

16

18

20

22

24

26

28

30 min 

1 

2 

3 

4 

6 

7 

8 9 

10

12

13

11 

5 

5

XBridge Glycan BEH Amide, 130Å, 2.5 µm XP and 3.5 µm Columns and Standards

[ CARE AND USE MANUAL ]

f. Scalable UPLC and HPLC BEH Glycan 
Column Offerings
Waters Glycan BEH Amide chemistry offerings are available 
in three highly scalable particle sizes that address UPLC 
(i.e. 1.7 µm) and HPLC-based (2.5 µm XP and 3.5 µm) 
application needs. (See Waters ACQUITY UPLC Glycan 
BEH Amide, 130Å, 1.7 µm Columns Care and Use 
Manual, literature code: 720003042EN.) Figure 2 clearly 
demonstrates the comparatively shorter analyses time with 
improved component resolution as the particle size of the 
XBridge Glycan BEH Amide Column chemistry is decreased. 
For this single variable study, the same low system dispersion/
volume ACQUITY UPLC System was used with all three 
columns since use of different LC systems with different 
system volumes would affect the final recorded results.

III. COLUMN USE
To ensure the continued high performance of XBridge Glycan 
BEH Amide Columns, observe the following guidelines:

a. Sample Preparation 
Sample impurities often contribute to column contamination. 
Samples should be free of particles before injection into 
the system. 

In most separations it is preferable to prepare the sample in the 
gradient initial composition. However, the labeled glycans are 
often insoluble in the high acetonitrile concentrations which 
typify HILIC initial conditions. Since small volume injections 
are being made, the sample diluents may contain higher 
aqueous content (e.g. 50%) than the initial composition.
Note: A mixture of acetonitrile and dimethylformamide is recommended 

when analyzing Waters RapiFluor-MS Labeled glycans (see: GlycoWorks 

RapiFluor-MS N-Glycan Kit Care and Use Manual p/n: 715004793EN).
If the sample is not dissolved in the mobile-phase or solvent 
combinations specified in this manual, ensure that the 
sample, solvent, and mobile phases are miscible in order 
to avoid sample and/or buffer precipitation. Preparation of  
labeled glycans could involve one or two steps of solid-phase 
extraction. As a result, protein precipitate has typically been 
removed. If not, remove protein particles by centrifugation at 
>10,000 rpm for more than 2 minutes.

15 16

ACQUITY Glycan BEH Amide, 130A 1.7 μm
2.1 x 150 mm

0.50 mL/min  

ACQUITY Glycan BEH Amide, 130A, 2.5 μm XP 

2.1 x 150 mm
0.34 mL/min  

7.4

5.0

ACQUITY Glycan BEH Amide, 130A, 3.5 μm

2.1 x 150 mm
0.24 mL/min   

10.3

1 2

3

4

5

6

7

89

10

11

12

13

14

8700 psi (Column, Max)

3300 psi (Column, Max)

900 psi (Column, Max)

30.0 min

44.3 min

62.1 min

Figure 2. Comparative scaled separations (based on column particle size differences) using Waters Glycan Performance Test Standard (p/n: 186006349) 

on ACQUITY UPLC H-Class System with ACQUITY UPLC Glycan BEH Amide, 130Å, 1.7 µm; XBridge Glycan BEH Amide, 130Å, 2.5 µm XP; and XBridge Glycan 

BEH Amide, 130Å, 3.5 µm Columns.

N-Acetylglucosamine

Manose

Galactose

Fucose

Sialic Acid

1

2

3

4

5

6,7

8

9

11

12

13

14

10

6

XBridge Glycan BEH Amide, 130Å, 2.5 µm XP and 3.5 µm Columns and Standards

[ CARE AND USE MANUAL ]

b. Operating pH Limits
The recommended operating pH range for the XBridge Glycan 
BEH Amide, 130Å Column is 3 to 8. A listing of commonly used 
buffers and additives is given in Table 2. Additionally, the column 
lifetime will vary depending on the operating temperature as well 
as the type and concentration of buffer used.

c. Solvents 
To maintain maximum column performance, use high quality 
chromatography grade solvents. If filtering, Acrodisc® filters 
are recommended. Solvents containing suspended particulate 
materials can damage the fluidic components of the UPLC 
System and will generally clog the inlet distribution frit of the 
column. This will result in higher operating pressure and 
poor performance. 

d. Pressure 
The XBridge Glycan BEH Amide, 130Å, 3.5 µm and 2.5 µm XP 
Columns will exhibit increased backpressure when operated 
in 80–100% aqueous mobile phases. As shown in the gradient 
table for Figure 1, the flow rate needs to be lowered when 
washing a glycan column. XBridge Glycan BEH Amide, 130Å, 
3.5 µm Column can tolerate pressures up to 5000 psi (345 bar 
or 34 Mpa) while the maximum operating pressure for the 
2.5 µm XP column is approximately 18,000 psi.
Note: Working at the extremes of pressure, pH and/or temperature will result 

in shorter column lifetimes.

e. Temperature 
Temperatures between 20 °C–90 °C are recommended for 
operating XBridge Glycan BEH Amide, 130Å Columns in order 
to enhance selectivity, lower solvent viscosity, and increase 
mass transfer rates. However, higher temperature will have a 
negative effect on lifetime that will vary depending on the pH 
and buffer conditions used. 

IV. TROUBLESHOOTING
The first step in systematic troubleshooting is comparing 
the column, in its current state, to the column when it was 
functioning properly. The method suggested in Section II for 
measuring plate count is an essential first step. This technique 
detects physical changes to the packed bed and chemical 
changes in the bonded-phase surface. The functional test with 
the 2-AB labeled Dextran Calibration Ladder (p/n: 186006841) 
or the Glycan Performance Test Standard (p/n: 186006349) 
may reveal more subtle changes in surface chemistry that 
affect the application.
There are several common symptoms of change in the column.
An increase in pressure is often associated with lost 
performance in the application. The first step in diagnosis is to 
ensure that the elevated pressure resides in the column rather 
than somewhere else in the system. This is determined by 
measuring pressure with and without the column attached to 
the instrument. If the system is occluded, the blockage should 
be identified and removed. If the pressure increase originates 
from the column, it is helpful to know whether the problem was 
associated with a single injection or whether it occurred over a 
series of injections. If the pressure gradually built up, it is likely 
that the column can be cleaned as described below (Section V). 
For future stability, it may be useful to incorporate a stronger 
regeneration step in the method. If a single sample caused the 
pressure increase, it likely reflects particulates or insoluble 
components. Cleaning is still an option, but using the more 
aggressive methods. The sudden pressure increase suggests 
that the user should consider some sample preparation, such 
as high speed centrifugation.
Loss of retention can reflect a change in the column surface 
chemistry. Before proceeding with diagnostic or corrective 
measures, check that the mobile phases have been correctly 
prepared and the correct method has been selected. Then 
repeat the plate count test and the glycan test standard.  

Table 2. Buffer Recommendations for Using ACQUITY Glycan BEH Amide, 130Å Columns from pH 3 to 8

Additive/Buffer

pKa

Buffer Range 

(±1 pH unit)

Volatility

Used for 

Mass Spec

Comments

Acetic acid

4.76

–

Volatile

Yes

Maximum buffering obtained when used with 
ammonium acetate salt. Used in 0.1–1.0% range.

Formic acid

3.75

–

Volatile

Yes

Maximum buffering obtained when used with 
ammonium formate salt. Used in 0.1–1.0% range.

Ammonium (acetate)

9.20

8.2–10.2

Volatile

Yes

Up to 100 mM.

Ammonium (formate)

9.20

8.2–10.2

Volatile

Yes

Up to 250 mM.

Triethylamine  

(as acetate salt)

9.7–11.7

Volatile

Yes

Used in the 0.1–1.0% range. Volatile only when titrated 
with acetic acid (not hydrochloric or phosphoric). 
Used as ion pair for DNA analysis at pH 7–9.

7

XBridge Glycan BEH Amide, 130Å, 2.5 µm XP and 3.5 µm Columns and Standards

[ CARE AND USE MANUAL ]

If both the plate count and glycan test show loss of retention, 
it is likely that a significant fraction of the bonded phase has 
been lost, and the column will require replacement. If the 
changes are small and reflected only for some glycans, one of 
the cleaning procedures may be effective.

Change in peak shape, resolution, or relative retention of peaks. 
Follow the same steps as for loss of retention (Section II).

Carryover and memory effects are defined as the appearance 
of the constituents of one sample in the next gradient analysis. 
First determine whether the column or the system is the 
source of carryover. Define a gradient method that includes an 
“internal gradient”. That is, the analytical gradient is repeated 
within a single method. If the glycan peaks appear in both 
gradients, at the same time after start, the carryover came 
from the column in what is often described as a “memory 
effect”. If the glycan peaks only appear when an injection is 
made, they likely originated from adsorption to some system 
component. In that case follow the instrument manufacturer’s 
recommendations. Memory effects as a source of carryover 
may be reduced or eliminated in several ways. First, raising 
the temperature of the separation reduces the possibility of 
non-specific adsorption. Second, memory effects may be 
more pronounced with steep gradients. Keep the gradient 
slope at 1% per column volume or less. Third, memory effects 
may be exacerbated by high flow rates. Reduce the flow rate 
by one half while doubling the gradient time to maintain a 
constant slope. Finally, apparent memory effects may actually 
reflect the solubility of the sample in the mobile phase. 
Reducing the amount injected may eliminate the effect.
Note: Useful general information on column troubleshooting problems may 

be found in HPLC Columns Theory, Technology and Practice, U.D. Neue, 

Wiley-VCH, 1997 (p/n: WAT038216), the Waters HPLC Troubleshooting Guide 

(Literature code # 720000181EN), or visit www.waters.com.

V. COLUMN CLEANING, REGENERATION, 

AND STORAGE 

a. Cleaning and Regeneration
Changes in peak shape, peak splitting, shoulders on the peak, 
shifts in retention, change in resolution, carryover, ghost peaks, 
or increasing backpressure may indicate contamination of the 
column. Choose a cleaning option that may be expected to 
dissolve the suspected contaminant.
1.  All cleaning procedures will be more effective at higher 

temperatures. It is reasonable to conduct cleaning at 70 °C.

2.  It may be useful to conduct cleaning procedures at one 

half the flow rate typically used with that column. In this 
way, the possibility of high pressure events is reduced.

3.  The first and simplest cleaning procedure is to run a 

series of gradients from 0–100% water. Be sure to reduce 
the flow rate for gradients with higher than 75% aqueous 
content. Columns of 150 mm length should be operated at 
250 µL per minute or less during washes. The gradients 
can be as short as 5 column volumes and 3–5 repetitions 
may be effective. 

4.  Regeneration steps and flushing procedures using 

100% aqueous mobile phase can help to maintain the 
optimal peak shape and selectivity of a HILIC separation.  
Additionally, an analyst can perform gradients with 
mobile phases containing 0.1% TFA as a means to 
maintain or recover the performance of a Glycoprotein 
BEH Amide column. TFA can be effective in cleaning 
a column’s stationary phase by both neutralizing and 
ion pairing contaminants that in their ionic form might 
otherwise strongly adsorb to a HILIC stationary phase. 

5.  Several different cleaning solutions may be injected to 

strip strongly adsorbed material or particulates from 
the column. Make the largest injection possible with 
the system configuration. With such strong cleaning 
solutions, it is best to disconnect the detector from the 
column and to direct the flow to waste.

6.  Flow reversal or backflushing is often suggested as part 

of a cleaning procedure. This should be reserved as a last 
resort. It may further damage the column or provide a 
short-lived improvement in performance.

b. Storage
For periods longer than four days at room temperature, store 
the column in 100% acetonitrile. Immediately after use with 
elevated temperatures and/or at pH extremes, store in 100% 
acetonitrile for the best column lifetime. Do not store columns 
in highly aqueous (<50% organic) mobile phases, as this may 
promote bacterial growth. If the mobile phase contained a 
buffer salt, flush the column with 10 column volumes of HPLC 
grade water (see Table 1 for common column volumes) and 
replace with 100% acetonitrile for storage. Failure to perform 
this intermediate step could result in precipitation of the 
buffer salt in the column or system when 100% acetonitrile is 
introduced. Completely seal the column to avoid evaporation 
and drying out the bed. 
Note: If a column has been run with a mobile phase that contains formate 

(e.g., ammonium formate, formic acid, etc.) and is then flushed with 100% 

acetonitrile, slightly longer equilibration times may be necessary when the 

column is re-installed and run again with a formate-containing mobile phase.

[ CARE AND USE MANUAL ]

Waters Corporation 
34 Maple Street 
Milford, MA 01757 U.S.A. 
T: 1 508 478 2000 
F: 1 508 872 1990 
www.waters.com

[ CARE AND USE MANUAL ]

Waters, The Science of What’s Possible, ACQUITY, UPLC, and XBridge are registered trademarks 
of Waters Corporation. RapiFluor-MS and BEH Technology are trademarks of Waters Corporation. 
All other trademarks are the property of their respective owners.

©2017 Waters Corporation. Produced in the U.S.A.  July 2017  Rev H  720004882EN  IH-PDF

VII. ORDERING INFORMATION (Partial listing. For more information, visit www.waters.com)

Product Description

Pore Size

Particle Size

Dimension

P/N

XBridge Glycan BEH Amide

130Å

2.5 µm

3.0 x 30 mm XP

186008038

XBridge Glycan BEH Amide

130Å

2.5 µm

3.0 x 75 mm XP

186008039

XBridge Glycan BEH Amide

130Å

2.5 µm

3.0 x 150 mm XP

186008040

XBridge Glycan BEH Amide

130Å

2.5 µm

VanGuard Pre-Column

186007262

XBridge Glycan BEH Amide

130Å

2.5 µm

2.1 x 50 mm XP

186007263

XBridge Glycan BEH Amide

130Å

2.5 µm

2.1 x 100 mm XP

186007264

XBridge Glycan BEH Amide

130Å

2.5 µm

2.1 x 150 mm XP

186007265

XBridge Glycan BEH Amide

130Å

2.5 µm

2.1 x 150 XP MVK

186007266

XBridge Glycan BEH Amide

130Å

2.5 µm

4.6 x 20 mm Guard, 2/pkg

186007267

XBridge Glycan BEH Amide

130Å

2.5 µm

4.6 x 50 mm XP

186007268

XBridge Glycan BEH Amide

130Å

2.5 µm

4.6 x 100 mm XP

186007269

XBridge Glycan BEH Amide

130Å

2.5 µm

4.6 x 150 mm XP

186007270

XBridge Glycan BEH Amide

130Å

2.5 µm

4.6 x 150 XP MVK

186007271

XBridge Glycan BEH Amide

130Å

3.5 µm

4.6 x 20 mm Guard, 2/pkg

186007272

XBridge Glycan BEH Amide

130Å

3.5 µm

4.6 x 50 mm

186007273

XBridge Glycan BEH Amide

130Å

3.5 µm

4.6 x 100 mm

186007274

XBridge Glycan BEH Amide

130Å

3.5 µm

4.6 x 150 mm

186007275

XBridge Glycan BEH Amide

130Å

3.5 µm

4.6 x 250 mm

186007276

XBridge Glycan BEH Amide

130Å

3.5 µm

4.6 x 150 MVK

186007277

XBridge Glycan BEH Amide

130Å

3.5 µm

2.1 x 10 mm Guard, 2/pkg

186007505

XBridge Glycan BEH Amide

130Å

3.5 µm

2.1 x 50 mm

186007502

XBridge Glycan BEH Amide

130Å

3.5 µm

2.1 x 100 mm

186007503

XBridge Glycan BEH Amide

130Å

3.5 µm

2.1 x 150 mm

186007504

Glycan Performance Test Standard

186006349

VI. CAUTIONARY NOTE
Depending on users application, these products may be 
classified as hazardous following their use and as such are 
intended to be used by professional laboratory personnel 
trained in the competent handling of such materials. 
Responsibility for the safe use and disposal of products rests 
entirely with the purchaser and user. The Safety Data Sheet 
(SDS) for this product is available at www.waters.com.