<div><div>Vědci z Ústavu organické chemie a biochemie AV ČR (ÚOCHB) vyvinuli průlomovou technologii pro efektivní vývoj aptamerů. Nová metoda propojuje několik biochemických a bioinformatických kroků a otevírá cestu k novým diagnostickým a terapeutickým aplikacím. Mezi ně patří například nositelné senzory schopné v reálném čase sledovat hladiny vybraných biomarkerů v těle a včas upozornit na blížící se zdravotní komplikace. Výsledky zveřejnil časopis Nature Communications.</div></div><div>Aptamery jsou krátké řetězce DNA nebo RNA, které dokážou velmi přesně rozeznat a navázat konkrétní cílovou molekulu. Podobně jako protilátky je lze využít k detekci nebo ovlivnění funkce těchto molekul. Na rozdíl od protilátek jsou však mnohem stabilnější, lze je vyrábět synteticky a přesně chemicky upravovat podle požadovaných vlastností. Díky tomu mohou získat vlastnosti, kterých nelze u protilátek dosáhnout.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_2_81e99b9900.jpg" alt="ÚOCHB: Nová technologie inovuje vývoj aptamerů, syntetických alternativ protilátek: Chemicky upravený DNA aptamer navázaný na cílový protein." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_2_81e99b9900.jpg 228w," sizes="100vw" width="1343" height="916">S rostoucí poptávkou po přesných a rychlých diagnostických nástrojích jsou aptamery v mnoha případech vhodnější než samotné protilátky. Jejich vývojový proces je však experimentálně a časově náročný a vyžaduje experty z několika vědních oborů. Tým vědců z ÚOCHB vedený dr. Markem Ondrušem a <a target="_blank" rel="noopener noreferrer" href="https://hocek.group.uochb.cz/">prof. Michalem Hockem</a> nyní vyvinul technologii, která celý vývojový proces výrazně zkracuje.„Vývoj aptameru často připomíná hledání jehly v kupce sena. Ve směsi bilionů různých DNA sekvencí se snažíme najít jedinou, která se silně a specificky váže na cílovou molekulu. Náš nový přístup umožňuje nejdřív nalézt ‚aptamerové rodiny‘ – stovky sekvencí s jistou podobností a následně identifikovat člena rodiny, který vykazuje nejlepší experimentální vlastnosti. Tento prvek přispívá ke zkrácení vývoje z několika měsíců na dny,“ říká Marek Ondruš.<blockquote>Video: <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=-aoGvOKqORo">Nová technologie inovuje vývoj aptamerů, syntetických alternativ protilátek&nbsp;</a></blockquote>Výzkumníci zároveň rozšířili možnosti využití aptamerů. Jejich stavební bloky totiž chemicky upravují – připojují k nim nové funkční skupiny, které můžeme přirozeně najít například v aminokyselinách. Takto vzniklé molekuly kombinují výhody DNA i protilátek: pořadí jednotlivých stavebních bloků je dané pořadím písmen v DNA a díky rozmanitosti připojených chemických modifikací se výrazně rozšiřují možnosti aptameru rozpoznávat biologicky významné cíle a napodobovat interakce typické pro proteiny, které jsou základem mnoha biologických procesů.&nbsp;Potenciál nové metody ukázali vědci na lidském inzulinovém receptoru, klíčovém proteinu zapojeném do regulace hladiny cukru v krvi. Vyvinuli aptamer, který se na receptor specificky váže, a pomocí kryoelektronové mikroskopie detailně popsali molekulární mechanismus vzájemné vazby.„Ukázali jsme, že chemicky zavedené funkční skupiny mají zásadní význam nejen pro rozpoznání a vytvoření komplexu aptameru s proteinem, ale také pro stabilizaci samotné struktury DNA aptameru. Naše výsledky poskytují nový pohled na to, jak lze pomocí chemicky upravených nukleových kyselin napodobovat vlastnosti proteinů,“ shrnuje Marek Ondruš.Technologie by v budoucnu mohla doplnit nebo v některých aplikacích dokonce nahradit diagnostické nástroje založené na protilátkách. Výzkumníci spolupracují na dalším rozvoji technologie se společností <a target="_blank" rel="noopener noreferrer" href="http://iocbtech.cz/">IOCB Tech</a>, která pomáhá přetavit objevy z ÚOCHB do praxe.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_9694c21671.jpg" alt="ÚOCHB/Tomáš Belloň: Nová technologie inovuje vývoj aptamerů, syntetických alternativ protilátek: Dr. Marek Ondruš, první autor studie, a prof. Michal Hocek, vedoucí vědecké skupiny Bioorganická a medicinální chemie nukleových kyselin, ÚOCHB" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_9694c21671.jpg 234w," sizes="100vw" width="2000" height="1335"><blockquote><h4>Původní článek</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-73676-y">Expedient single-round selection of hyper-modified aptamer targeting insulin receptor from over-represented dually nucleobase-modified DNA libraries</a></h4>Pablo Alberto Franco-Urquijo, Marek Ondruš, Jaroslav Kurfürst, Jana Škerlová, Irena Selicharová, Lucie Mužíková Čechová, Hana Šváchová, Alena Semerádtová, Anatolij Filimoněnko, Adéla Fejfarová, Jiří Homola, Tomáš Kouba &amp; Michal HocekNat. Commun., 2026<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41467-026-73676-y">https://doi.org/10.1038/s41467-026-73676-y</a>&nbsp;<h6>licencováno pod <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstrakt</h4>Objev funkčních nukleových kyselin z knihoven náhodných sekvencí se obvykle opírá o několik časově náročných iterativních kol in vitro selekce s nízkou mírou úspěšnosti. V této práci představujeme strategii selekce v jediném kole, která umožňuje rychlý screening několika nadreprezentovaných DNA knihoven s modifikovanými nukleobázemi a různých selekčních podmínek a dokáže identifikovat modifikované aptamery s vysokou afinitou. Dvojitá separace následovaná amplifikací eluovaných sekvencí, analýzou NGS a klastrováním umožňuje rychlou identifikaci kandidátních aptamerů. Screening modifikovaných DNA knihoven obsahujících modifikované adeninové a uracilové nukleotidy nesoucí hydrofobní aromatické fenylové a indolové skupiny vedl k vývoji aptameru, který se váže na lidský inzulinový receptor se subnanomolární afinitou a mimořádnou specificitou. Struktura stanovená kryoelektronovou mikroskopií odhaluje význam každé aromatické modifikace, a to buď při stabilizaci sekundární struktury, nebo při zprostředkování interakcí s povrchem proteinu. Tento přístup překonává hlavní omezení selekce aptamerů a má potenciál pro vysokokapacitní screening i urychlení vývoje aptamerů nové generace pro diagnostické nebo terapeutické aplikace.</div>

Nová technologie inovuje vývoj aptamerů, syntetických alternativ protilátek

<div><div>Nadace Experientia podpoří tříletým start-up grantem vycházející hvězdu organické chemie Petru Vojáčkovou. Mladí talentovaní vědci Tereza Edlová a Ladislav Prener vycestují na zahraniční stáže.</div></div><div><div><a target="_blank" rel="noopener noreferrer" href="https://www.experientia.cz/start-up-granty/vyvijime-nove-strategie-pro-stavbu-molekul">Petra Vojáčková </a>z <a target="_blank" rel="noopener noreferrer" href="https://www.uni-muenster.de/en/">University of Münster</a>&nbsp;založí svoji výzkumnou skupinu na <a target="_blank" rel="noopener noreferrer" href="https://www.muni.cz/">Masarykově univerzitě</a> v Brně, kterou od ledna 2027 podpoří start-up grant Nadace Experientia ve výši 6 600 000 Kč. Se svým týmem bude vyvíjet nové strategie pro stavbu molekul tak, aby vznikala výhradně jejich žádoucí zrcadlová podoba.<a target="_blank" rel="noopener noreferrer" href="https://www.experientia.cz/zahranicni-staze/chceme-zefektivnit-vyzkum-v-oblasti-novych-materialu-i-leciv">Tereza Edlová </a>z <a target="_blank" rel="noopener noreferrer" href="https://www.vscht.cz">VŠCHT Praha</a> díky stipendiu ve výši 1 260 000 Kč vycestuje na roční stáž na britskou <a target="_blank" rel="noopener noreferrer" href="https://www.manchester.ac.uk">University of Manchester</a> do skupiny prof. Igora Larrosy, kde se bude věnovat projektu, který může zefektivnit výzkum v oblasti materiálů i léčiv.<a target="_blank" rel="noopener noreferrer" href="https://www.experientia.cz/zahranicni-staze/nase-metoda-by-mohla-urychlit-vyvoj-novych-leku">Ladislav Prener </a>z <a target="_blank" rel="noopener noreferrer" href="https://www.uochb.cz/cs">ÚOCHB AV ČR</a> získal stipendium ve výši 1 200 000 Kč a zamíří na německou <a target="_blank" rel="noopener noreferrer" href="https://www.rwth-aachen.de/cms/~a/root/?lidx=1">RWTH Aachen University</a> do skupiny prof. Leonoriho. Na své roční stáži bude vyvíjet metodu, která by mohla urychlit vývoj nových léků.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Nadace_Experientia_Nadace_Experientia_zna_drzitelku_start_up_grantu_a_stipendisty_pro_rok_2026_c96fdf9a4f.jpg" alt="Nadace Experientia: Nadace Experientia zná držitelku start-up grantu a stipendisty pro rok 2026" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Nadace_Experientia_Nadace_Experientia_zna_drzitelku_start_up_grantu_a_stipendisty_pro_rok_2026_c96fdf9a4f.jpg 234w," sizes="100vw" width="600" height="400"><blockquote>Nadace Experientia od roku 2012 podpořila už 27 nadějných mladých vědkyň a vědců, kteří díky stipendiu dostali šanci vycestovat na roční stáž na špičková zahraniční vědecká pracoviště. Díky programu start-up grantů si mohlo dalších 6 mladých vědkyň a vědců založit po návratu ze zahraničí vlastní výzkumnou skupinu v ČR. Celkem už na podporu mladých vědkyň a vědců vyčlenila Nadace Experientia více než 73 milionů korun.</blockquote></div></div>

Nadace Experientia zná držitelku start-up grantu a stipendisty pro rok 2026

V týdnu od 29. června na Vás čekají v oblasti kapalné fáze a především HPLC a LC/MS následující webináře.<blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://www.lcms.cz/webinars">👉 DETAILY A REGISTRACE V SEKCI WEBINÁŘE</a></h4></blockquote><blockquote>👉 Vyhledávejte a filtrujte v největší globální databázi s více než 5 500 GC, HPLC, MS a spektroskopickými webináři.</blockquote><h5>1. Thermo Fisher Scientific: Insights from an IC Specialist: To Be Suppressed or Not Suppressed?</h5><ul><li>Út, 30.6.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6310">Registrace</a></li></ul>Zjistěte, jak technologie supresorů ovlivňuje citlivost, selektivitu, spolehlivost i nároky na údržbu v iontové chromatografii. Webinář představí výhody i omezení potlačené a nepotlačené vodivostní detekce a nabídne praktické tipy pro řešení běžných problémů.<h5>2. SCIEX: Advancing Proteomics with ZenoTOF: From Single-Cell Analysis to Plasma Biomarker Discovery</h5><ul><li>Út, 30.6.2026 11:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6311">Registrace</a></li></ul>Objevte možnosti hmotnostní spektrometrie ZenoTOF pro proteomiku jednotlivých buněk i analýzu plazmatických biomarkerů. Přednáška ukáže, jak vysoké rozlišení a citlivost urychlují biologický výzkum a objevování nových biomarkerů.<h5>3. Agilent Technologies: Measuring Diverse Samples with UV/VIS Spectrometers and Integrating Spheres</h5><ul><li>Út, 30.6.2026 20:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6052">Registrace</a></li></ul>Naučte se správně měřit difúzní transmitanci a reflektanci pomocí UV/VIS spektrometrů s integrační koulí. Webinář představí praktické využití systému Cary 60 při analýze široké škály materiálů.<h5>4. Waters Corporation: Informatics Series #33: Tips, Tricks and Best Practices</h5><ul><li>Čt, 2.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6315">Registrace</a></li></ul>Interaktivní lekce zaměřená na software Empower představí užitečné funkce, klávesové zkratky a osvědčené pracovní postupy, které pomohou zrychlit každodenní práci a zvýšit efektivitu zpracování chromatografických dat.<h5>5. Waters Corporation: Unlocking Therapeutic Peptide Characterisation (GLP-1RAs) with Cyclic IMS</h5><ul><li>Čt, 2.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6313">Registrace</a></li></ul>Zjistěte, jak cyklická iontová mobilita rozšiřuje možnosti charakterizace terapeutických peptidů a analogů GLP-1. Webinář ukáže přínosy této technologie pro vývoj léčiv, kontrolu kvality i regulatorní dokumentaci.<h5>6. Agilent Technologies: Spectral Flow Cytometry Masterclass – Session 1</h5><ul><li>Pá, 3.7.2026 07:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6316">Registrace</a></li></ul>První část odborné série představí principy spektrální průtokové cytometrie, návrh experimentů a praktické aplikace v imunologii, onkologii i výzkumu vakcín.

Webináře LabRulezLCMS týden 27/2026

Webináře

Popularizace

Věda a výzkum

Video

Článek

Podporujeme mladé vědkyně a vědce z oboru organické, bioorganické a medicinální chemie na zahraničních stážích i zde v ČR díky tzv. start-up grantům Nadace Experientia.

Nadace Experientia

ÚOCHB AV ČR je přední mezinárodně uznávaná vědecká instituce, jejímž hlavním posláním je základní výzkum v oblasti chemické biologie a medicinální chemie, organické a materiálové chemie, chemie přírodních látek, biochemie a molekulární biologie, fyzikální chemie, teoretické chemie a analytické chemie.

Ústav organické chemie a biochemie AV ČR

Vše ze světa analytické chemie na jednom místě. Propojujeme lidi při řešení jejich problémů. U nás naleznete všechny potřebné informace snadno, rychle a přehledně. Přestaňte hledat a začněte nacházet.

LabRulez

Agilent Technologies

PerkinElmer

SelectScience

Waters Corporation

In this webinar, join our low flow LC expert who will review micro and nano LC fundamentals, and present a selective approach to trap and column strategies to increase method performance. They will provide chromatographic examples on forward and backward trap elute strategies, trap selectivity comparisons, and micro and nano LC column selectivities, and will explain how to find the best selectivity strategy for your application.
<h5>Key learning objectives</h5>
<ul>
<li>Understand the current state of micro and nano LC and hardware advances</li>
<li>Understand trap and elute – forward and backward comparison</li>
<li>Discover trap and column selectivity strategies</li>
<li>Learn how to find the best selectivity for your application</li>
</ul>
<h5>Who should attend?</h5>
<ul>
<li>Any scientist who is interested in improving and expanding their micro and Nano LC performance and would like to increase their knowledge of current trap and column strategies and hardware advancements</li>
</ul>
<blockquote>
Presenter: Roxana Eggleston-Rangel (Senior Applications Scientist, Phenomenex)
</blockquote>
<blockquote>
Presenter: Dora Wells (SelectScience)
</blockquote>

SelectScience: What You Need to Know: Micro and Nano Low Flow LC Essentials

What You Need to Know: Micro and Nano Low Flow LC Essentials

In this webinar we will review micro and nano LC fundamentals, and present a selective approach to trap and column strategies to increase method performance.

<h3>Two-dimensional liquid chromatography (2D-LC): A tutorial focusing on best practice</h3>
In recent years, 2D-LC has evolved from a niche technology to one that is in demand, especially when it comes to the analysis of complex samples, or hidden or co-eluting compounds. Whether you are starting out with 2D-LC or want to refresh your knowledge, this two-part webinar series will give you a comprehensive introduction to the technique, including best practices.
<h4>Session 1: Key concepts of 2D-LC</h4>
Learn about the four modes of 2D-LC: heart-cutting, multiple heart-cutting, selective and full comprehensive, and find out how they relate conceptually. Plus, get advice on how to choose complementary separations for the first and second dimension (RP, IEX, HILIC, etc.), how to optimize cycle time, and how to maximize second dimension sensitivity.
<h5>Who should attend?</h5>
New and experienced 2D-LC users, scientists, and lab managers or technical decision makers in pharma, biopharma, food, energy &amp; chemicals, environmental, material, forensics, and life science research
<blockquote>
Presenter: Dr. Dwight Stoll (Professor of Chemistry, Gustavus Adolphus College. St. Peter, MN, USA)
</blockquote>
<blockquote>
Dr. Dwight Stoll completed his undergraduate work at Minnesota State University, Mankato, in plant biology and biochemistry. Upon graduation in 1999, he took a job in industry as a research technician with ZirChrom Separations, Inc. For his graduate work, he studied with Professor Peter Carr in the Chemistry Department at the University of Minnesota from 2001–2007, where he worked on the development of fast, comprehensive two-dimensional liquid chromatography.
</blockquote>
<blockquote>
In the fall of 2008, Stoll was hired as an Assistant Professor in the Chemistry Department at Gustavus Adolphus College, where he mainly teaches quantitative and instrumental analysis courses. In 2014, he was promoted to Associate Professor, and in 2018, he was promoted to Professor. Currently, he is co-chair of the Chemistry Department. Active research projects in his laboratory touch upon most aspects of multi-dimensional separation methodologies, including optimization strategies, characterization of selectivity in reversed-phase HPLC, instrument development, and applications in biopharmaceutical analysis.
</blockquote>
<blockquote>
Stoll is the author or co-author of more than 62 peer-reviewed publications and three book chapters in the area of separation science, is a named co-inventor on four granted patents, and has instructed numerous short courses in two-dimensional liquid chromatography. In 2011, he was the recipient of LCGC’s Emerging Leader in Chromatography Award. In 2014, he added to The Analytical Scientist’s list of ‘Top 40 Under 40’ analytical scientists. In 2017, he received the Georges Guiochon Faculty Fellowship and an Agilent Technologies Thought Leader Award, which will support research in his laboratory on the development of 2D-LC methodologies for biopharmaceutical analysis.
</blockquote>

Agilent Technologies: Two-dimensional liquid chromatography (2D-LC): A tutorial focusing on best practice

Key concepts of 2D-LC

Learn about the four modes of 2D-LC: heart-cutting, multiple heart-cutting, selective and full comprehensive, and find out how they relate conceptually.

<h3>Two-dimensional liquid chromatography (2D-LC): A tutorial focusing on best practice</h3>
In recent years, 2D-LC has evolved from a niche technology to one that is in demand, especially when it comes to the analysis of complex samples, or hidden or co-eluting compounds. Whether you are starting out with 2D-LC or want to refresh your knowledge, this two-part webinar series will give you a comprehensive introduction to the technique, including best practices.
<h4>Session 2: Solutions and best practices for 2D-LC</h4>
Get an overview of 2D-LC instrumentation for multi-heart cutting and comprehensive 2D-LC. Gain tips on how to deal with discrepancies between mobile phase dimensions and what to consider for column re-equilibration, detection and minimising additional column dispersion. Plus, hear small molecule application examples from different industries and method development case studies. You can also ask questions to the speaker at the end of this part.
<h5>Who should attend?</h5>
New and experienced 2D-LC users, scientists, and lab managers or technical decision makers in pharma, biopharma, food, energy &amp; chemicals, environmental, material, forensics, and life science research
<blockquote>
Presenter: Dr. Dwight Stoll (Professor of Chemistry, Gustavus Adolphus College. St. Peter, MN, USA)
</blockquote>
<blockquote>
Dr. Dwight Stoll completed his undergraduate work at Minnesota State University, Mankato, in plant biology and biochemistry. Upon graduation in 1999, he took a job in industry as a research technician with ZirChrom Separations, Inc. For his graduate work, he studied with Professor Peter Carr in the Chemistry Department at the University of Minnesota from 2001–2007, where he worked on the development of fast, comprehensive two-dimensional liquid chromatography.
</blockquote>
<blockquote>
In the fall of 2008, Stoll was hired as an Assistant Professor in the Chemistry Department at Gustavus Adolphus College, where he mainly teaches quantitative and instrumental analysis courses. In 2014, he was promoted to Associate Professor, and in 2018, he was promoted to Professor. Currently, he is co-chair of the Chemistry Department. Active research projects in his laboratory touch upon most aspects of multi-dimensional separation methodologies, including optimization strategies, characterization of selectivity in reversed-phase HPLC, instrument development, and applications in biopharmaceutical analysis.
</blockquote>
<blockquote>
Stoll is the author or co-author of more than 62 peer-reviewed publications and three book chapters in the area of separation science, is a named co-inventor on four granted patents, and has instructed numerous short courses in two-dimensional liquid chromatography. In 2011, he was the recipient of LCGC’s Emerging Leader in Chromatography Award. In 2014, he added to The Analytical Scientist’s list of ‘Top 40 Under 40’ analytical scientists. In 2017, he received the Georges Guiochon Faculty Fellowship and an Agilent Technologies Thought Leader Award, which will support research in his laboratory on the development of 2D-LC methodologies for biopharmaceutical analysis.
</blockquote>

Solutions and best practices for 2D-LC

Get an overview of 2D-LC instrumentation for multi-heart cutting and comprehensive 2D-LC.

High Performance Liquid Chromatography (HPLC) is an important technique used in Analytical Laboratories for the identification and quantitation of different analytes/compounds in samples. It is the most versatile and dependable chromatographic technique for quality control.
In this seminar, we will cover the complete LC workflow, starting from mobile phase to detection, the different parts involved in this flow path and the different consumables associated with each module of LC system and their importance in acquiring chromatographic results.
<h5>Please join us in this webinar to learn about:</h5>
<ul>
<li>Introduction to LC workflow</li>
<li>Sample flow path and the respective modules involved from Mobile phase to Detection.</li>
<li>Importance of small consumable parts of each module for smooth running of LC system.</li>
<li>How to enhance productivity with new innovative technology products in day-to-day chromatography analysis.</li>
</ul>
<h5>Who Should Attend:</h5>
<ul>
<li>Users of Liquid chromatography in Pharma, Academia &amp; Applied Markets</li>
<li>Analytical scientists, chemists, and lab managers trying to enhance their lab productivity.</li>
</ul>
<blockquote>
Presenter: Dr. Ramaprasad Ganni (Technical Support Specialist, Agilent Technologies)
</blockquote>
<blockquote>
Ramaprasad Ganni has 21 years of experience in the field of Chromatography and Column chemistries. Working with Agilent technologies India, since 2008 in Chemistries and Supplies division. Previously Worked for Elsevier GmbH, and Millipore India Pvt, Ltd. Responsible for Workflow solutions development, providing technical support, and trainings. Experienced in Sample prep, HPLC, GC, Mass spec and GPC Chromatography technologies .
</blockquote>

Agilent Technologies: Mobile Phase to Detector - Complete Workflow

Mobile Phase to Detector - Complete Workflow

In this seminar, we will cover the complete LC workflow, starting from mobile phase to detection

A UV-VIS spectrometer is a powerful and versatile tool for material analysis. It is capable of providing very quick information of sample composition and concentration for quality control in addition to indicating how samples change versus time or temperature.
In this webinar, we will go over what is happening when we take a UV-VIS measurement, discuss how measurement parameters can improve our data collection, and look at applications like concentration and kinetics.
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Presenter: Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)
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Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.
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Agilent Technologies: Understanding Your UV-Vis Measurements

Understanding Your UV-Vis Measurements

What is happening when we take a UV-VIS measurement, how measurement parameters can improve our data collection, and look at applications.

In this educational webinar series, we will go back to the basics of chromatography, mass spectrometry, data analysis and troubleshooting. Understanding the importance of the basics will enable you to be more effective in your laboratory. During each session we will cover a specific aspect of your LC or LC/MS system:
<ul>
<li>The theory of chromatography and requirements for great separations</li>
<li>The basics of your Liquid Chromatograph (LC): Pumps, injectors, and detectors</li>
<li>An Introduction to mass spectrometry (MS) systems</li>
<li>Considerations with System Suitability</li>
<li>Troubleshooting your LC and MS systems</li>
</ul>
<h3>PART 2 | LC SYSTEMS</h3>
<ul>
<li>LC System Components: Pumps and Injectors</li>
<li>LC Detectors – Which one is right for your application?</li>
</ul>
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Presenter: Melanie Peguese-Richards (Technical Support Specialist, Waters Corporation)
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Melanie Peguese-Richards is a Technical Support Specialist at Waters, based in Georgia and supporting customers across the Southeastern United States. Prior to joining Waters, she worked for 18 years as a Scientist developing and validating LC, GC, and other analytical methods in small molecule pharma.
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HOST: Kieron Faherty, Ph.D. (Separations and Mass Spectrometry Field Marketing Manager, Waters Corporation)
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Kieron Faherty is the Separations and Mass Spectrometry Field Marketing Manager for the Americas at Waters Corporation. He received his B.Sc. (Hons) in Chemistry from the University of Reading (Reading, Berkshire, UK) and his doctorate in Chemistry from the University of Massachusetts (Amherst, MA). He has held several field-based positions at Waters, most recently as a Field Applications Specialist focused on biopharmaceutical applications using Time-of-Flight mass spectrometry instrumentation.
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HOST: Alexandra (Allie) Ahern (Informatics Product Marketing Manager, Americas Field Marketing, Waters Corporation)
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Allie Ahern has been with Waters for 10 years and is the Informatics Product Marketing Manager within the Americas Field Marketing Organization. Prior to Allie’s current role, she has held other roles at Waters including Service Marketing Product Manager, Implementation Project Manager, and Field Service Engineer.
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Waters Corporation: Fall Back to Basics - Part 2: LC Systems

Fall Back to Basics - Part 2: LC Systems

PART 2 topics: LC System Components: Pumps and Injectors. LC Detectors – Which one is right for your application?

In this educational webinar series, we will go back to the basics of chromatography, mass spectrometry, data analysis and troubleshooting. Understanding the importance of the basics will enable you to be more effective in your laboratory. During each session we will cover a specific aspect of your LC or LC/MS system:
<ul>
<li>The theory of chromatography and requirements for great separations</li>
<li>The basics of your Liquid Chromatograph (LC): Pumps, injectors, and detectors</li>
<li>An Introduction to mass spectrometry (MS) systems</li>
<li>Considerations with System Suitability</li>
<li>Troubleshooting your LC and MS systems</li>
</ul>
<h3>**PART I | CHEMISTRY **</h3>
<ul>
<li>Theory of Chromatography</li>
<li>Creating Great Separation Methods</li>
</ul>
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Presenter: Chris Collins, Ph.D. (Chemistry Account Manager, Waters Corporation)
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Chris Collins joined Waters in 2018 as a Chemistry Account Manager covering Eastern North Carolina, providing support to customers on all chemistry and consumable products. He has over twenty years’ experience in the pharmaceutical industry with a focus on LC/MS Method Development.
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HOST: Kieron Faherty, Ph.D. (Separations and Mass Spectrometry Field Marketing Manager, Waters Corporation)
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Kieron Faherty is the Separations and Mass Spectrometry Field Marketing Manager for the Americas at Waters Corporation. He received his B.Sc. (Hons) in Chemistry from the University of Reading (Reading, Berkshire, UK) and his doctorate in Chemistry from the University of Massachusetts (Amherst, MA). He has held several field-based positions at Waters, most recently as a Field Applications Specialist focused on biopharmaceutical applications using Time-of-Flight mass spectrometry instrumentation.
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HOST: Alexandra (Allie) Ahern (Informatics Product Marketing Manager, Americas Field Marketing, Waters Corporation)
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Allie Ahern has been with Waters for 10 years and is the Informatics Product Marketing Manager within the Americas Field Marketing Organization. Prior to Allie’s current role, she has held other roles at Waters including Service Marketing Product Manager, Implementation Project Manager, and Field Service Engineer.
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Waters Corporation: Fall Back to Basics - Part 1: Chemistry

Fall Back to Basics - Part 1: Chemistry

PART 1 topics: Theory of Chromatography. Creating Great Separation Methods.

Fall Back to Basics - Part 1: Chromatography chemistry 

This presentation is the first in a series that will introduce you to the most widely used analytical technique in the world – high performance liquid chromatography (HPLC). HPLC is a separation technique that allows you to separate, identify and quantitate the components in a sample. The objective of this session is to provide you with an entry level understanding of this technique. Topics to be discussed include chromatographic theory, the different modes of HPLC, a generic look at HPLC instrumentation, and where HPLC is used.
In later sessions in this series some of the items discussed under these topics (i.e., columns, detectors and troubleshooting) will be discussed in greater detail.
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Presenter: Pamela A. Perrone (Senior Principal LC Technical Education Specialist, Perkinelmer)
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Pam has been with PerkinElmer for almost 39 years. She had the opportunity to work as a summer intern and was offered full-time employment after completing her Ph.D. in Analytical Chemistry at the University of Rhode Island. Currently Pam is in the role she finds most fulfilling and challenging which is as the global support and trainer for both service and customers for LC and chromatography data handling. To help someone learn and be successful in their job is very rewarding.
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Pam has a passion for three things (besides LC, of course!): her family, the UConn women’s basketball team and Broadway. She believes there is a Broadway song that fits almost every occasion.
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PerkinElmer: Liquid Chromatography 2022 Learning Series

Introduction to HPLC

This presentation is the first in a series that will introduce you to the most widely used analytical technique in the world – HPLC.

One of the key features in developing a HPLC chromatographic method is the selection HPLC column. It is the focus of this presentation to review the key factors that go into HPLC column selection. These factors include column length, column diameter, particle size of the column packing media, pore size of the column packing media, column type and stationary phase of the packing media.
We explain how column selection can be a match in developing an optimized separation of analyte classes and ultimately how this selection can help ensure a robust separation method. We show how practical compromises can be made in selecting the best column for required separation.
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Presenter: Matthew Przybyciel, Ph.D. (Research and Development Leader, PerkinElmer)
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Matthew has a PhD in Analytical Chemistry from the University of South Carolina. The focus of his research was the development of new stationary phases for capillary gas chromatography. In the late 1990’s he worked in ES Industries focusing its efforts exclusively on chromatography column products for HPLC and SFC where Matt was Vice President and Technical Director for chromatography products. He has successfully developed and commercialized a number of chromatographic stationary phases and columns for high performance chromatographic separations for HPLC and SFC.
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In 2021, ES Industries became part of PerkinElmer, Inc. and Matthew now is product development leader at PerkinElmer. As part of his work, he has also published a number of articles on the development and application of new stationary phases for GC, HPLC and SFC.
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Introduction to LC Column Selection

One of the key features in developing a HPLC chromatographic method is the selection HPLC column.

In this session of our LC Learning Series, we will explore the most common detectors for High Performance Liquid Chromatography (HPLC). The presentation will focus primarily on optical detection, such as UV/Vis, Photodiode Array, Fluorescence and Refractive Index detectors, however other HPLC detectors will also be considered.
The objective of this presentation is to provide a basic understanding of the theory of operation, advantages and disadvantages of these detectors, and what to consider when choosing a detector for a particular analysis. Common application examples will be given.
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Presenter: Adrian Garcia Sega (Application Scientist, PerkinElmer)
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Adrian joined PerkinElmer last year as anapplication scientist developing strategic applications for the LC product portfolio. Previously, he spent eight years in higher-education teaching chemistry and forensic science to graduate and undergraduate students. His teaching was focused on integrating technology into the classroom and developing active-learning activities and experiments to improve student comprehension and engagement.
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Choosing the Right Detector: A look at the most common detectors for HPLC

The presentation will focus primarily on optical detection, such as UV/Vis, PDA, Fluorescence and RI detectors, other HPLC detectors will also be considered.

LCMS webináře se zaměřením na Akademie/Principy - strana 27