Introduction to LC Column Selection

One of the key features in developing a HPLC chromatographic method is the selection HPLC column. It is the focus of this presentation to review the key factors that go into HPLC column selection. These factors include column length, column diameter, particle size of the column packing media, pore size of the column packing media, column type and stationary phase of the packing media.

We explain how column selection can be a match in developing an optimized separation of analyte classes and ultimately how this selection can help ensure a robust separation method. We show how practical compromises can be made in selecting the best column for required separation.

Presenter: Matthew Przybyciel, Ph.D. (Research and Development Leader, PerkinElmer)

Matthew has a PhD in Analytical Chemistry from the University of South Carolina. The focus of his research was the development of new stationary phases for capillary gas chromatography. In the late 1990’s he worked in ES Industries focusing its efforts exclusively on chromatography column products for HPLC and SFC where Matt was Vice President and Technical Director for chromatography products. He has successfully developed and commercialized a number of chromatographic stationary phases and columns for high performance chromatographic separations for HPLC and SFC.

In 2021, ES Industries became part of PerkinElmer, Inc. and Matthew now is product development leader at PerkinElmer. As part of his work, he has also published a number of articles on the development and application of new stationary phases for GC, HPLC and SFC.