Agilent MassHunter Quantitative Data Analysis - Batch Table, Compound Information Setup, Calibration Curve and Globals Settings
Agilent MassHunter
Quantitative Data
Analysis
Presenters: Howard Sanford
Stephen Harnos
MassHunter Quantitation:
Batch Table, Compound
Information Setup,
Calibration Curve and
Globals Settings
1/17/2018
MassHunter Webinar Series
1
MassHunter Quantitative Software
Review and Quant Method Optimization
Topics
• Brief Review
• Batch Table Navigation
• Compound Information
- Data review and manual integration
• Calibration Curve
• Working in Compounds At a Glance
• Method Editor Globals Setting
1/17/2018
MassHunter Webinar Series
2
Review
Three major views in MassHunter Quantitative Analysis.
• Batch At a Glance
• Method Editor
• Compounds At a Glance
Handling MS/MS data QQQ and QTOF.
Handling accurate mass data TOF and QTOF.
1/17/2018
MassHunter Webinar Series
3
MassHunter Quantitative Software
Batch-at-a-Glance View
1/17/2018
MassHunter Webinar Series
4
Batch Table
Calibration Curve
Compound Information
Method
Tasks
MassHunter Quantitative Software
Method Editor View
Method > Edit menu or F10 key
1/17/2018
MassHunter Webinar Series
5
Method Table
Sample
Information
Compound Information
MassHunter Quantitative Software
Compounds at a Glance View
View > Compounds-at-a-Glance
1/17/2018
MassHunter Webinar Series
6
Editing a Quantitation Method for TOF
Mass Extraction Setup
Method > Edit > Advanced Tasks > Mass Extraction Setup
Allows for a mass range for the extraction of the accurate mass (MZ).
Available MZ Extraction Window Units
1/17/2018
MassHunter Webinar Series
7
Editing a Quantitation Method for QTOF
Compound Setup & Mass Extraction Setup
QTOF is a combination of MS/MS and accurate mass data
Name
– Compound name
TS
– time segments may be multiple
Transition
– Precursor ion Product ion scan
Scan
– Product Ion
Type
– Target, ISTD, Surrogate or Matrix Spike
Precursor Ion
– mass of the ion
Product Ion
– mass of the ion for the target ion to monitor
RT
– retention time of compound of interest
Ion Polarity
– usually positive
Criteria
– Close RT, Close RT with Qualifiers, Greatest Response or
Greatest Q-Value
1/17/2018
MassHunter Webinar Series
8