Improving high-throughput Top-Down proteomics using a modified hybrid quadrupole-ultra-high-field-Orbitrap mass spectrometer
Postery | 2017 | Thermo Fisher Scientific | ASMSInstrumentace
LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap
ZaměřeníProteomika
VýrobceThermo Fisher Scientific
Klíčová slovaftms, abundance, dda, top, down, sliding, relative, deconvolution, high, masses, esi, full, window, xtract, proteins, improved, protein, min, exactive, workflow, time, medium, beat, intact, monoisotopic, proteoform, redundant, respect, mass, orbitrap, fragmentation, modified, base, infusion, advanced, algorithms, collision, method, npv, beats, average, energy, throughput, hybrid, quadrupole, igh, ribosomal, spectrometer, mixtures, peak
Podobná PDF
Top-down Characterization of Monoclonal Antibody on an Orbitrap Fusion Lumos Tribrid Mass Spectrometer
2016|Thermo Fisher Scientific|Postery
Poster Note 64778 Top-down Characterization of Monoclonal Antibody on an Orbitrap Fusion Lumos™ Tribrid Mass Spectrometer Seema Sharma, Stephane Houel, Christopher Mullen, Chad Weisbrod, Romain Huguet, John Syka, Dave Horn, Jonathan Josephs, Jae Schwartz, Vlad Zabrouskov Thermo Fisher Scientific, San…
Klíčová slova
abundance, abundancefragmentation, fragmentationantibody, antibodyintact, intactetd, etdrelative, relativemab, mabtop, topdown, downheavy, heavymonoclonal, monoclonaldeglycosylated, deglycosylateduvpd, uvpdpulses, pulsessequence
A Complete Workflow Solution for Intact Monoclonal Antibody Characterization Using a New High-Performance Benchtop Quadrupole-Orbitrap LC-MS/MS
2012|Thermo Fisher Scientific|Postery
A Complete Workflow Solution for Intact Monoclonal Antibody Characterization Using a New High-Performance Benchtop Quadrupole-Orbitrap LC-MS/MS Zhiqi Hao,1 Yi Zhang,1 David Horn,1 Seema Sharma,1 Shiaw-Lin Wu, 2 Irene Ae-Ning Lin, 3 Yi-Hsuan Pan, 3 Ya-Fen Yang, 3 and Andreas F.…
Klíčová slova
mab, mababundance, abundanceintact, intactrelative, relativeexactive, exactivehcd, hcdspectrum, spectrumfile, fileorbitrap, orbitrapenvelope, envelopemass, masscharge, chargedeconvolution, deconvolutiondown, downantibody
Combination of Bottom-Up and Top-Down Characterization of Biologics Using a High Throughput Capable Workflow in Proteome Discoverer Software
2015|Thermo Fisher Scientific|Postery
For bottom-up experiments, the monoclonal antibodyy Rituximab was denatured for 30 min in 7 M Urea and 50 mM Tris HCL at pH 8.00. The sample was reduced with 5 mM 37°C and alkylated by addition of 10 mM IAA…
Klíčová slova
top, topdown, downbottom, bottomfigure, figuresequest, sequestbyonic, byonicndance, ndanceabun, abunterminal, terminalrelative, relativeprosight, prosighttthe, tthezoominto, zoomintokfl, kfldiscoverer
Complete characterization of monoclonal antibodies under native and denaturing conditions
2021|Thermo Fisher Scientific|Aplikace
APPLICATION NOTE 73885 Complete characterization of monoclonal antibodies under native and denaturing conditions Kristina Srzentić1, Eugen Damoc2, Kai Scheffler3, Tom Buchanan4, Krisztina Radi4, Jennifer Sutton1 Thermo Fisher Scientific, 1 Cambridge, Massachusetts, US 2 Bremen, Germany 3 Germering, Germany 4 Hemel…
Klíčová slova
intact, intacttrastuzumab, trastuzumabnative, nativemass, masshcd, hcdmab, mabsubunit, subunitthermo, thermodown, downdeconvolution, deconvolutionscientific, scientificsubunits, subunitspeptide, peptidemiddle, middleheavy