Is 2D HPLC the Answer for You?

2D HPLC is the selective transfer of a fraction or fractions from one column to another for further separation. It can be used in a variety of situations including:

• Further resolution of a complex mixture that can’t be separated on a single column (1D chromatography)
• Sample cleanup, removing matrix or interfering compounds
• Trace enrichment

While using reversed phase in both dimensions is the most common combination for 2D HPLC, it has the lowest orthogonality. We’ll look at how combining orthogonal techniques, like IEX and RP can help you achieve optimum resolution and discuss the advantages and disadvantages of these combinations.

Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)

Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.