It’s Not All About the Column: The Role of the Mobile Phase and Your Instrument
Choosing the right column is a critical part of successful chromatography but that’s not all it takes.
We’ll discuss:
Choosing a buffer
- Options
- pH ranges
- Volatile vs. non-volatile
Organic
- Choices
- Compatibility with aqueous component and sample
We’ll also talk about how our instrument can contribute to our success or defeat our efforts without us knowing why.
Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)
Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.
