Highly Multiplexed Targeted Proteomics Acquisition on a TIMS-QTOF

The new tims-TOF pro-mass spectrometer offers improved capabilities for the targeted acquisition. The prm-PASEF method uses the multiplexing capability provided by the trapped ion mobility separation, allowing more than 200 peptides to be monitored over a 30 min liquid chromatography separation. Compared to conventional parallel reaction monitoring (PRM), precursor ions are accumulated in the TIMS cells and separated according to their shape and charge before eluting into the Q-TOF part of the mass spectrometer.

Using these improved mass spectrometric capabilities, we detected and quantified 216 isotope-labeled synthetic peptides (AQUA peptides) spiked in HeLa human cell extract with limits of quantification of 17.2 amol for some peptides.

Presenter: Prof. Dr. Gunnar Dittmar (Group leader proteomics of cellular signalling, Luxembourg Institute of Health, Department of Infection and Immunity)

Prof Dr Gunnar Dittmar is the Head of proteomics of cellular signalling group at the LIH’s Quantitative Biology Unit. He has a strong background in proteomics, bioinformatics, chemistry and biochemistry. He completed a PhD in cell biology at the DKFZ (German Cancer Research Centre), in Heidelberg in 1997, followed by six years at the Harvard Medical School in Boston (USA), first as a postdoctoral fellow and later as an instructor of Cell Biology.Back to Germany, he was recruited as a group leader at the Max Delbru¨ck Center for Molecular Medicine (MDC), in Berlin in 2003. In 2007, he established the Mass Spectrometry Core Unit at MDC, and in 2014 he additionally built up the Proteomics Unit of the Berlin Institute of Health (BIH), a translational research centre resulting from the joint forces of MDC and the Charite´ – medical school of the Freie and Humboldt University, Berlin. During these years, he further intensified his knowledge in proteomics and mass spectrometry techniques, oriented towards a clinical perspective. Since 2011 he is a visiting professor at the Technion University in Haifa, Israel.In 2016 he was recruited to the Luxembourg institute of health (LIH) where he created the new proteomics research group, a bioinformatics group and built the new multi-centre LuxGen NGS sequencing platform (a joined effort of the LIH and LNS – Laboratoire National de Sante´). Starting in 2018 he worked on setting up the new quantitative biology unit, which was officially inaugurated in 2019. The unit consists of ten different technology-oriented groups and platforms, spanning from bioinformatics to in vivo imaging. Since 2019 he has been appointed as an affiliated professor for proteomics at the University of Luxembourg.

Moderator: Dr. Martin Graf-Utzmann (Wiley Analytical Science, Lab Automation & Equipment)

Dr. MartinGraf-Utzmann studied Chemistry at the FAU Erlangen-Nuremberg, worked on his PhD. Thesis at the Max-Planck-Institute for Coal Research in Mülheim an der Ruhr. Since 2008 he works for Wiley, starting in the books editorial team and since2013 in the B2B group.