Back to Basics 2: Mobile Phase Selection In HPLC & UHPLC
It has an effect on retention time of analytes, selectivity between these and the peak efficiency. Consideration should be taken not only to the type of solvents used, but also the concentration of these and whether an isocratic run or a gradient run is most suitable. Additives can also impact the resolution, retention and peak shape of sample analytes and so careful choice of buffer must also be made.
We discuss all of these topics, looking at the most commonly used organic modifiers for reversed phase U/HPLC in both isocratic and gradient methods and how these can impact your separation and run times.
A number of topics will be covered during the seminar:
- Solvent options for U/HPLC
- Method development solvent considerations
- Isocratic and gradient chromatography
- Influence of buffer salt and pH on separations
Presenter: Gemma Lo
Gemma Lo is a key part of the business development team at Avantor, and is responsible for leading a wide range of HPLC and UHPLC training events and seminars across Europe. Gemma has over a decade of experience working in the technical support team, troubleshooting chromatography methods, recommending suitable column chemistries, and supporting the vast range of HPLC and UHPLC columns in the portfolio. Originally a Biochemist, her focus areas include the analysis of biomolecules by various chromatographic techniques, and the analysis of polar analytes.
