Workflows for robust and sensitive separation of mAbs, intact proteins at subunit and peptide level using capillary chromatography by LC-MS
Unsplash/National Cancer Institute: Workflows for robust and sensitive separation of mAbs, intact proteins at subunit and peptide level using capillary chromatography by LC-MS
Learn how to utilize superior resolving power to achieve in depth characterization of therapeutic antibodies at protein, subunit and peptide level using the novel capillary Thermo Scientific™ MabPac™ RP column in in either stand-alone or integrated EASY-Spray design coupled to LC-MS.
Here we will focus on:
- A novel column chemistry specifically designed in capillary format for the reproducible separation of therapeutic antibodies and their subunits
- Bottom up applications to monitor protein attributes such as site occupancy, disulfate mapping at the peptide level
Key learning objectives:
- Learn how to easily set-up the hyphenation between the new capillary MAbPac RP column and mass spectrometry for intact protein and bottom-up proteomics applications
- Extend your capability in attaching nano and capillary columns to reliable and robust emitters
Who should attend:
- Proteomics, biopharma and academic researchers interested in the characterization of intact proteins by top-down and bottom-up proteomics
- Researchers whose sample volumes are small and who seek to use the capillary chromatography to increase the sensitivity and high throughput of mass spectrometry coupled workflows
Presenter: Dr. Mike Baynham (Senior Product Manager, LC Columns, Thermo Fisher Scientific)
Mike Baynham is a Senior Product Manager and the Manager of the Product Management Team responsible for the Thermo Scientific LC column portfolio. Mike has worked for Thermo Fisher Scientific in Runcorn, UK since 2015. Mike has a doctorate in capillary separation techniques coupled to mass spectrometry from the University of Nottingham and has more than 25 years of experience of working with liquid chromatography.
Presenter: Dr. Ken Cook (EU Bio-Separations Expert, Thermo Fisher Scientific)
Dr. Ken Cook has 30 years of experience supporting liquid and ion chromatography applications with Dionex and then Thermo Fisher Scientific. Dr. Cook currently supports customers with bio-separations, focusing on biotherapeutics. Previously Dr. Cook was a lecturer in biochemistry at the University of Newcastle-Upon-Tyne, UK where he focused on protein biochemistry.