Taming the Viral Beast: Applying LC and LC-MS Technologies to the Analysis of rAAV Viral Vectors
Recombinant adeno-associated viral (rAAV) vectors have emerged as the leading gene delivery platform for gene therapy and genetic vaccination. For this promising gene delivery vector to fully realize its therapeutic or prophylactic potential, a rational design strategy is desired. The current challenges in viral vector manufacturing robustness, scale up, and productivity will need to be addressed to turn this mode of treatment into an accessible global benefit. To meet this goal, reliable analytical technologies to help establish a structure-function relationship, guide the development of manufacturing processes, and assess the quality of clinical materials have become increasingly relevant and urgent.
In this upcoming webinar, hear from Ximo Zhang, senior scientist at Waters, to discover the challenges associated with analyzing AAV samples, and discover how LC and LC-MS workflows can help solve these setbacks, including those that employ size-exclusion chromatography (SEC), anion-exchange chromatography (AEX), protein reversed phase LC-MS, and peptide mapping LC-MS based analyses.
Watch this webinar to learn
- How to use SEC for AAV aggregation and titer analysis
- Optimize IEX for AAV empty/full capsids analysis
- Develop LC/MS-based method for AAV proteins and peptides analysis
Who should attend?
This webinar will provide insights for researchers interested in LC and LC-MS methods for gene therapy or nucleic acid-based vaccine products within these organizations:
- Pharmaceutical
- Contract research organizations
- Academia
- Government laboratories
- Analytical scientists analyzing AAV or related biomolecules
- Laboratory managers and directors responsible for advancing development and manufacture AAV-based modalities
Presenter: Ximo Zhang (senior scientist at Waters)
Ximo Zhang is a Senior Scientist in the Biopharma scientific group at Waters. Ximo graduated from Purdue University with a Ph.D. in Analytical Chemistry from Professor Mary Wirth’s group in 2016. After graduation, Ximo joined Waters to continue her pursuit in contributing to the advancement in separation science. In the past few years, Ximo has developed a series of novel LC and LC-MS based methods and workflows for the analysis of biopharmaceuticals, including methodologies for released glycans, fusion proteins, synthetic peptides, and oligonucleotides. Ximo’s research currently focuses on the analysis of adeno-associated virus using LC and MS technologies.
Presenter: Stephan M. Koza, PhD (Principal Scientist, Waters Corporation)
Stephan has worked for Waters for 9 years and leads an applications group at Waters that has a primary focus on the use of UPLC, HPLC, LC-MS, and sample preparation chemistries and columns for the analysis of biomolecules. Prior to joining Waters he had nearly twenty years of experience with biopharmaceutical characterization and analytical method development.
