Beyond C18 and Phenyl: Expanding Selectivity with Biphenyl Columns Built for UV and pH Robustness
Resolve your separation challenges
Biphenyl stationary phases are increasingly used to resolve separations that challenge conventional C18 and phenyl chemistries, particularly for aromatic analytes and positional isomers. However, traditional biphenyl columns can suffer from elevated UV-detected bleed, limited pH stability, and inconsistent batch performance.
This presentation shows how a trifunctionally bonded biphenyl phase on a hybrid ethylene-bridged particle delivers balanced hydrophobic and aromatic selectivity with stable performance from pH 1.5–10 and ≥10× lower UV bleed. Inert column hardware further improves recovery of metal-sensitive analytes. Pharmaceutical, bioanalytical, and food/environmental examples demonstrate robust, reproducible separations.
What you will learn
- Recognize limitations of traditional biphenyl phases affecting robustness and quantitation.
- Explain trifunctional biphenyl design enabling balanced selectivity, wide pH stability.
- Apply biphenyl selectivity and inert hardware to improve recovery.
Who should attend
This webinar is designed for you if you are a Pharma Method Developer, R&D Method Developer, or Bioanalytical Scientist looking to optimize and future proof your analytical methods.
Speaker: Chris Collins, Ph.D. (Principal Product Marketing Manager, Waters Corporation)
Chris Collins, Ph.D. earned his Ph.D. in Analytical Chemistry from North Carolina State University in 2014. He has over 20 years experience in the pharmaceutical industry with a focus on LC/MS Method Development.
Chris started working at Waters Corporationin2018 as a Chemistry Account Manager for North Carolina/South Carolina. In 2023, he transitioned to the role of Principal Product Marketing Manager for ACQUITY UPLC columns and MaxPeak Premier Column Technology.
