Fit-for-purpose workflows for inositol stereoisomer profiling in dietary supplements and foods using single and tandem mass spectrometry

Join us for a free webinar on Analysis of Inositol Stereoisomer Profiling in Dietary Supplements and Foods

Inositol plays a key role in regulating numerous metabolic pathways and hormonal signaling processes. Maintaining a balanced profile of inositol stereoisomers in the human body is critical for supporting key biological functions and may help reduce the risk of certain health disorders. The aim of this webinar is to present analytical procedures capable of simultaneously separating and quantifying free inositol stereoisomers in both simple matrices, such as dietary supplements, and complex matrices, such as milk, cereal, and infant formula. An existing Hydrophilic Interaction Liquid Chromatography (HILIC) protocol was systematically optimized to achieve adequate resolution and reliable separation of seven inositol stereoisomers (allo-, muco-, epi-, D-chiro-, neo-, myo-, and scyllo-inositol) using a UPLC™ BEH™ Amide Column. Two different sample preparation procedures and two mass spectrometry (MS) techniques (ACQUITY™ QDa™ II Mass Detector and Xevo™ TQ-S cronos Mass Spectrometer) were developed for the analysis of the simple and complex matrices, respectively. Details of method development and analytical performance will be discussed.

Presenter: Jinchuan Yang, Ph.D. (Principal Scientist, Waters Corporation)

Dr. Jinchuan Yang is a Principal Scientist Waters Corporation, and a subject matter expert in food and environmental applications, polymer chromatography, and LC and MS technologies. Since he joined Waters in 2002, his roles at Waters have mainly focused on application development, market development, and support for R&D and training. He is an active participant in technical stakeholder groups at AOAC International and USP, serving as an expert panel member in AOAC SPIFAN and participating in review, recommendation and muti-lab testing of AOAC methods.

Presenter: Dimple Shah (Moderator | Principal Food and Materials Market Development Manager, Waters Corporation)

Dimple Shah has a M.S. degree in Organic Chemistry from St. Xavier’s college, Gujarat University, India. She also has a M.S. degree in Chemistry from University of Massachusetts at Lowell. She is working at Waters Corporation, Milford as a Principal Market Development Manager for Food and Material Science. In her previous journey she was a Principal Scientist at Waters and worked in Genotoxic impurity and Food and Environment market. She was responsible for development of analytical methods for contaminants in food and water using LC and Mass spectrometers. Her areas of expertise are LC, tandem quadruple, high resolution mass spectrometry and various chromatographic and mass spectrometry software. She has published several application notes on polar pesticides, pesticides, veterinary drugs, mycotoxins, sugars, sweeteners, vitamins, dyes and NDSRIs.