Understanding Quantification Algorithms in Proteome Discoverer software

This free Thermo Scientific Proteome Discoverer Software online workshop is suitable for both novice and intermediate users. Participants will also have the opportunity to ask questions related to handling quantitation in Proteome Discoverer software.

Topics

The workshop will cover the following topics in Proteome Discoverer 3.2:

• Abundance value
• Quantification nodes in Processing and Consensus Workflows
• Minora Feature Detector
  • Steps in feature detection
  • Parameters
• Feature Mapper
  • Processing steps in the Feature Mapper node
  • Parameters
• Precursor Ions Quantifier
  • Parameters (shared peptides, shared quan results, replicate threshold, modification handling)
  • Normalization
  • Scaling
  • Missing value imputation
• Protein ratio calculation