Introducing the Orbitrap Excedion Pro Hybrid MS
The presentation introduces the new Thermo Scientific Orbitrap Exploris Pro, highlighting its expanded capabilities for biopharmaceutical and protein therapeutic characterization. Building on the robustness and flexibility of earlier Orbitrap systems, the new instrument adds electron transfer dissociation (ETD) with optional supplemental activation (EThcD) and an extended mass range up to 12,000 m/z, enabling improved intact protein analysis and detection of large aggregates at low abundance. These enhancements support deeper sequence coverage, confident PTM identification, isobaric amino acid differentiation, and disulfide bond mapping. Additional improvements include a new ion source to reduce source fragmentation, higher scan speeds, increased throughput, and advanced workflows such as hybrid DIA and adaptive retention time correction. The biopharma edition is particularly suited for complex modalities such as ADCs, bispecific antibodies, and long peptides, providing high sensitivity, accuracy, and confidence for critical analytical decisions in biologics characterization.
Learning points:
- Orbitrap Exploris Pro advances biopharma analysis: Added ETD/EThcD and an extended mass range (up to 12,000 m/z) enable better characterization of complex biologics, including low-level aggregates and variants
- ETD delivers higher confidence results: ETD/EThcD improves sequence coverage, PTM localization, disulfide mapping, and differentiation of isobaric amino acids
- Improved performance and workflows: Reduced source fragmentation, higher speed and throughput, and new workflows (hybrid DIA, adaptive RT) enhance sensitivity and data quality across applications
Who should attend?
- Biopharma and protein therapeutics scientists: Researchers working on monoclonal antibodies, bispecifics, ADCs, and complex biologics who need confident structural characterization and PTM analysis
- Mass spectrometry and proteomics experts: Users focused on advanced MS/MS strategies, ETD-based workflows, and high-sensitivity peptide and protein analysis
- Analytical development and characterization teams: Scientists responsible for method development, comparability, and quality-relevant characterization in biopharmaceutical pipelines
This webinar is a recording from the Omics innovations for the biopharmaceutical industry seminar on December 11, 2025 in Strasbourg.
Cannot attend on February 12? Please register anyway to automatically receive the link to the recording.
Presenter: Claire Dauly (Senior Manager, Product Management, Thermo Fisher Scientific)
