Targeted Proteomics: SysQuan: Advancing Absolute Quantitative Proteomics Across Biofluids, Tissues, and Multiomics

SysQuan leverages stable isotope-labeled mice as internal standards to overcome challenges in quantitative proteomics, enabling absolute protein quantitation with high reproducibility, multiplexicity and scalability across plasma, tissues, and implementation in multiomics workflows. 

Using targeted multiple reaction monitoring on the newest Agilent 6495D system coupled to Evosep chromatography, the approach facilitates streamlined sample processing and broad protein profiling. It enables high-throughput quantitation of hundreds of liver proteins in one-pot (or One-Tip) digestion and single injection experiments while also integrating metabolomics and lipidomics to analyze hundreds of plasma proteins together with up to 50 biochemical classes, more than 300 small molecules, and more than 900 lipids. This cost-effective method enhances cross-laboratory comparisons, and large-scale biomarker discovery and validation, setting a new standard for quantitative proteomics.

Presenter:  Christoph Borchers (Professor, Department of Oncology; Director, Segal Cancer Proteomics Centre McGill University)

Professor in the Department of Oncology at McGill University, and holds the Segal Chair in Molecular Oncology. His research involves proteomics and metabolomics technologies for clinical diagnostics and structural proteomics. He is also head of a newly established Omics laboratory for personalized medicine and health at Skoltech, Moscow, Russia.