Mastering Single Cell Proteomics: Daily Excellence with μPAC Neo Plus Trap-and-Elute Workflow

Achieve reliable, high-performance single-cell proteomics—every day.

Join Jeff Op de Beeck (Thermo Fisher Scientific) for a deep dive into a practical single-cell proteomics workflow designed to deliver exceptional sensitivity, precision, and robustness. This session showcases how the Thermo Scientific™ μPAC™ Neo Plus trap-and-elute setup empowers researchers to perform daily single-cell analysis with unmatched depth and reproducibility.

What You Will Learn

• How to achieve reliable low-flow LC-MS performance suited for single-cell studies
• Advantages of μPAC™ micro Pillar Array columns (50 cm) in high-sensitivity workflows
• Implementing trap-and-elute approaches with the Vanquish™ Neo UHPLC
• Maximizing identification depth and quantitative precision using Orbitrap™ Astral­­™ / Exploris™ systems with FAIMS™ interface
• Practical tips for building a robust, daily-use workflow for ultra-low-sample proteomics

Topics Covered

• Low-flow LC-MS
• Single-cell proteomics
• μPAC Neo Plus trap-and-elute workflow
• Variable-flow nano/capillary LC methods
• High-performance MS with Orbitrap Astral/Exploris + FAIMS

Who Should Attend

• Proteomics scientists and mass spectrometrists seeking higher sensitivity and throughput in single-cell LC-ESI-MS analysis
• Method developers and LC-MS operators interested in nano/capillary workflows, trap-and-elute strategies, and precise low-nanoliter injections on the Vanquish Neo UHPLC
• Core facility managers and lab leaders looking to implement robust, reproducible workflows for daily high-productivity operation
• Single-cell biology researchers working with limited sample material who need consistent, end-to-end workflows from isolation to data interpretation

We’ll conclude with a live Q&A session—bring your questions!

Presenter: Jeff Op de Beeck (Staff Scientist R&D, Thermo Fisher Scientific)