Advancing LC-MS Biomarker Analysis with High Throughput Affinity Enrichment
Accurate and sensitive quantification of biomarkers in complex biological matrices is a persistent challenge in clinical proteomics and translational research. Low-abundance target proteins or peptides are often masked by highly abundant interfering species, compromising the sensitivity and selectivity of standard liquid chromatography-mass spectrometry (LC-MS) assays.
This webinar will discuss the application of an innovative, high-throughput affinity enrichment technique using PhyTip® columns in the analysis of high-density lipoproteins (HDL), a critical cardiovascular disease biomarker. We will explore how this technique creates a rapid, automatable method with high analytical sensitivity and improved accuracy and precision for the analysis of these complex particles, significantly surpassing the limitations of traditional, time-consuming methods.
By attending this webinar you will:
- Understand the principles, resin options, and practical applications of the PhyTip® format for rapid and scalable analyte capture
- Learn how to seamlessly integrate automated PhyTip®-based enrichment for LC-MS analysis to achieve robust and reproducible, high-throughput results
- Examine a successful application of high-throughput affinity enrichment using PhyTip® columns.
Who Should Attend:
This presentation is essential for analytical scientists, proteomics researchers, and clinical chemists developing sensitive and selective mass spectrometry-based assays for quantifying protein and peptide biomarkers.
Presenter: Timothy Collier, PhD (Scientific Director of R&D - Quest Cardiometabolic Center of Excellence at the Cleveland HeartLab®)
Dr. Timothy Collier is Scientific Director of Research & Development for the Quest Cardiometabolic Center of Excellence at Cleveland HeartLab, where his responsibilities include overseeing the development and deployment of new assays in the clinical laboratory. Dr. Collier has over 21 years of research laboratory experience as an analytical biochemist and 18 years of experience specializing in mass spectrometry-based proteomics. He received his BS in chemistry and PhD in bioanalytical chemistry and biotechnology from North Carolina State University and was a post-doctoral research scholar in the division of molecular oncology and hematology at Washington University in St. Louis School of Medicine/Barnes-Jewish Hospital in St. Louis, MO.
Presenter: Lee Hoang, PhD (Sr. Field Application Scientist - Biotage®)
Lee Hoang was deeply inspired as an undergraduate at Yale University during the height of RNA crystallography and ribozyme research. While dabbling in field work studying tick-borne diseases, and making DNA oligos at the core facility, Hoang was lured by RNA’s siren call and was accepted into the UCSC Center for Molecular Biology of RNA under the guidance of Professor Harry Noller. Study of the ribosome can be summarized as 7 years to understand how 5 amino acids and 2 nucleotides support the RNA World Hypothesis. Hoang transitioned to industry science and joined PhyNexus prior to the company’s acquisition by Biotage. As a life science executive Hoang spent over 19 determined years applying biochemical reductionism to management and business. His current role as Senior Field Application Scientist sees him interfacing with scientists to solve problems and creating efficient workflows. Hoang is a part of a team responsible for 7 patents and multiple product launches.
