Green chemistry for versatile and comprehensive small oligonucleotide therapeutic analysis
Join us for an informative webinar on the updated characterization of RNA-based therapeutics with green chemistry utilising LC-MS and compliant Thermo Scientific Chromeleon CDS. Learn about our automated intact deconvolution and reporting workflow in Chromeleon software that enhances the efficiency and accuracy of RNA therapeutic analysis.
In this webinar, we will showcase:
- Method for reversed-phase LC without ion pairing or HFIP coupled to a high-resolution MS and MS/MS method (HRMS) for sensitive characterization of several different single-stranded antisense oligonucleotides (ASOs).
- Evaluation and comparability of the new RP method to that of traditional ion pairing reagent methods
- Detailed impurity characterization of therapeutic ASO RNA as well as sequencing of the of the full-length product (FLP) in the same multi attribute analytical method.
- A new GLP compliant workflow and report setup in Chromeleon that enables automated, high throughput intact LC-MS analysis for ASO RNA.
Learning Objectives:
- Learn how to analyse ASO RNA impurities by LC-MS with a fast and sensitive method to confirm identification, purity assessment, as well as sequence confirmation.
- Understand the benefits of high-resolution mass spectrometry in quality control of oligonucleotides, and how this compares with the implementation of this method using single quadrupole and triple quadrupole instruments.
- Discover best practices and tips & tricks for oligonucleotide analysis by LC-MS, including software solutions and implementation of the analysis in a compliant manner.
ASO RNA and siRNA are a powerful new class of therapeutic modalities, which can target previously untreatable disease states. Robust and easy to implement analytical methods have proved difficult to develop. This has been made more challenging by the initiative to remove HFIP, a common reagent in the eluent system, from use in new methodologies. Most approaches for this remain inadequate for comprehensive characterization. Here we describe a GLP compliant answer to this problem that has proven up to the task and extremely versatile in the ability to characterize a range of different oligonucleotides. Many new drug candidates can appear in manufacturer pipelines and in clinical trials. To ensure high quality, safety, and efficacy, analytical methods need to be developed quickly and be easy to perform. The breakthroughs described here will aid in getting these new medicines to the market.
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Presenter: Dr. Ken Cook (BioPharma Applications Support Expert, Thermo Fisher Scientific)
Moved to industry from a University lectureship in Biochemistry, University of Newcastle upon Tyne, following 7 years’ experience in academia. Now with over 35 years’ experience with Thermo Fisher Scientific using a wide range of analytical equipment and software. Publications range from metabolomics, proteomics to large biopharmaceutical drug analysis. Current job title is the European manager for the BioPharma expert support group. This involves the support of Bio-Pharmaceutical applications to characterise protein and oligonucleotide based bio-therapeutics. This involves ongoing collaborations with the Biopharmaceutical industry and academia. Characterization methods of Bio-therapeutics include, monoclonal antibodies, peptides, AAV analytical development, small and large RNA analysis including sequence mapping of mRNA by LC/HRMS.
