French Horseracing Lab (LCH) and the Utilization of LC-MS/MS for Steroid Hormones Analyses
Anabolic steroids are banned substances in equine races and sports. These steroids are mainly analyzed for doping control in urine and plasma samples, which allows to detect several forms of steroids including parent drugs, their esters, metabolites conjugated forms or not. The analysis of equine hair for the detection of anabolic steroids allows to extend their detection window.
In order to expose the detection of anabolic steroids, the laboratory continues to constantly improve its methods. For instance, in urine, the well documented and robust GC-MS/MS method had been transferred to an LC-MS/MS method, with the aim of saving time, money and being able to observe steroids under different forms.
LC-MS/MS screening methods are also used for the detection of steroids in equipe blood and hair, with sample preparation based on SLE extraction (different from SPE used for urine samples). Those methods are in constant evolution, and their improvements of performances always in search, thanks to the use of new generation of mass spectrometers such as Xevo TQ Absolute.
Who should attend:
- Professionals in analytical chemistry and equine sports regulation
What you will learn:
- How LC-MS/MS is transforming steroid detection in urine, plasma, blood, and hair
- The shift from GC-MS/MS to LC-MS/MS for improved efficiency and cost-effectiveness
- Sample preparation techniques including SLE and SPE
Presenter: Florian BAGILET (Head of Anabolic Steroids department MSc Analytical Chemistry, Université Paris-Saclay Laboratoire des Courses Hippiques, GIE-LCH, France)
M. Florian BAGILET is Head of anabolic steroids department at Laboratoire des CoursesHippiques for three years, actively developing analytical methods used for screening andconfirmatory analysis in complex matrices such as equine urine, blood ans hair, using mainlyhigh-performance liquid chromatrography methods hyphenated to mass spectrometrytechnologies such as LC-MS/MS (QqQ and HRMS).
Laboratoire des Courses Hippiques is ISO 17025 accredited and a reference laboratory of theInternational Federation of Horseracing Authorities (IFHA) and the International Federation forEquestrian Sports (FEI) which allowed the lab to be appointed to analyse all equestriansamples for PARIS 2024 Olympics.
In an area facing constant new challenges, Florian leads the developments and advances insteroid analysis, including technological tranfers and new sample preparation methodologies.
