Best Practices for HPLC Column Care and Maintenance

Proper care of your HPLC column is essential for consistent, high-quality results. In this webinar, we will cover best practices to maintain your column in optimal condition. Topics include proper connection techniques, mobile phase considerations, column equilibration, cleaning procedures, and correct storage methods. We will also address some common column related issues and offer troubleshooting strategies to help resolve them. Whether you're working with Reverse Phase, HILIC, SEC, or IEX columns, being aware of your column’s technical specifications and applying careful handling can significantly extend the column’s performance and lifetime and have a lasting impact on your chromatography.
Presenter: Jean Lane (Application Engineer, Agilent Technologies, Inc.)
Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.
