New Clarity OTX Pro – Case Study: GalNAc siRNA Quantitation by SPE-LC-MS
In this webinar, we will present a solid phase extraction (SPE) method for quantifying GalNAc-conjugated siRNA in plasma and liver matrices, and will explore how optimizing key method parameters can significantly boost recovery rates and enhance analytical performance.
Key Learning Points
- Review key sample pre-treatment method strategies for the successful and improved recovery of GalNAC-conjugated siRNA in plasma and liver tissue.
- Explore how find how Clarity OTX Pro SPE strategies differ when extracting ASOs versus GalNAc-conjugated siRNA oligonucleotides.
- Learn how to choose the right Clarity OTX Pro well plate format for oligonucleotide extraction in complex matrices.
Who Should Attend
- Anyone looking to begin or improve extraction of GalNAc-conjugated siRNA duplex oligonucleotides from plasma or liver samples using solid phase extraction (SPE).
Speaker: Mihir Thakar (Product Manager, Phenomenex)
Mihir holds a master’s degree in Biotechnology. He has over a decade of experience working with various biopharma companies in the characterization of therapeutic peptides, proteins, and oligonucleotides. He has been associated with Phenomenex for more than 7 years, serving in various functions including as a Business Development Manager for Biologics. At present, he has been working as a Global Product Manager for the biopharma segment.
Speaker: Roxana Eggleston-Rangel (Senior Applications Scientist, Phenomenex)
Roxana joined Phenomenex in 2018 as an Applications Scientist and is currently working with the applications team to produce analytical solutions for the biopharma industry. Her current work focuses on characterization of mRNA, sgRNA and siRNA. Prior to Phenomenex, Roxana worked for Caltech and the University of Southern California developing LM-CS workflows for the proteomics community. She received her bachelor’s and master’s degree in Chemistry and Biochemistry from California State University, Los Angeles.
