SCIEX summit Day two: Advanced strategies in analytical science
Join us in October to hear first-hand how scientists and researchers from around the globe are driving innovation through analytical science—as they share insights on the latest trends, advanced workflows, and techniques that are helping to shape the future of food safety, environmental health, pharmaceutical and biopharmaceutical breakthroughs, and cutting-edge techniques in biomedical and omics research.
This year’s summit will spotlight:
- Future-focused solutions: Explore how next-generation technologies and approaches are redefining the boundaries of analytical capability.
- Advanced strategies: Dive into cutting-edge methodologies that are transforming laboratories across pharma, biotech, environmental, food, and clinical domains.
- Emerging trends: Gain foresight into trends driving the future of analytical science
Whether you're a scientist, researcher, or industry innovator, this is your moment to be part of the conversations. Secure your spot today and be among the first to access exclusive content, speaker announcements, and agenda previews. Be ready for what’s next in analytical excellence.
Day two: Advanced strategies in analytical science
- Discover advanced strategies and techniques that are reshaping analytical workflows and delivering measurable impact in mass spectrometry today.
- Explore advanced mass spectrometry approaches that deliver precise characterization of complex IgA O-glycosylation, enhancing analytical confidence for glycoprotein studies.
- Discover how the Echo MS+ system can streamline cell line development with an application for small-scale bioreactor monitoring with rapid, robust, and high-throughput analysis.
- Confirmed speakers include: Talus Bio, Novartis, J&J Innovative Medicines, and the University of Utah.
Agenda-at-a-glance
Welcome and introduction to advanced strategies in analytical science
- Susan Darling (Senior Director, Adjacent & Transformational Technologies, SCIEX)
Evaluating quantitative figures of merit for ZT Scan DIA and ZenoSWATH DIA
- Daniele Canzani (Senior Scientist II, Talus Bio)
Mass spectrometry-based proteomics depends on measurements that are both quantitatively accurate and precise. We used matrix-matched calibration curves to assess quantitative figures of merit for each peptide measured in a proteome across various acquisition settings on a ZenoTOF 8600 system in order to identify optimal settings for quantitative accuracy and precision.
Key takeaways from the presentation:
- LOD and LOQ values are measured using Zeno SWATH and ZT scan DIA modes and using multiple gradient lengths for high-throughput analysis
- Assessment of quantitative figures of merit enables selection of optimized acquisition settings
- Peptides were identified and quantified using various search tools to evaluate differences between software approaches
Identification and mode of action of covalent inhibitors using the Echo® MS+ system
- Dan Selle (Research Assistant, Texas A&M)
We have been developing methods to elucidate modes of action and implement high-throughput screening of covalent inhibition against priority targets from Mycobacterium tuberculosis using the Echo® MS+ system. Our first study utilizes the Echo® MS+ system to characterize the kinetics of a fast-acting covalent inhibitor against Pks13-AT (PMID: 40739353). We have since expanded our use of the EchoMS+ to include robust high-throughput screening of covalent libraries against multiple Mtb targets.
Characterizing O-glycosylation in the hinge region of immunoglobulin A (IgA) by bottom-up and middle-up MS with EAD
- Patrick Schindler (Laboratory Manager, Novartis Institutes for BioMedical Research)
IgA nephropathy has been associated with galactose-deficient IgA1, but the identification of sites that contain only N-acetylgalactosamine (GalNAc) rather than the typical O-GalNAc-Gal-Sia (1/2) remains unclear. This uncertainty is partly attributable to the clustering of different sites within a short sequence region and the proline-rich repeat structure of the hinge sequence. This study used the benchtop ZenoTOF 7600 system, employing both electron-activated dissociation (EAD) and CID techniques, to characterize IgA hinge O-glycosylation.
Key takeaways from the presentation:
- Fine-tuned EAciD fragmentation can accurately localize O-glycans on proline-rich peptides
- An LC-MRMHR strategy combined with Zenotrapping improves sensitivity
- High-quality LC separation is important for reliable MSMS identification of isomer mixtures
End to end small scale bioreactor monitoring using the Echo® MS+ system
- Michael Poltash (Senior Scientist, J&J Innovative Medicines)
Bioreactor monitoring for product quality, with live monitoring of small-scale bioreactors and spent media analysis provides feedback and control during cell culture. Discover how the Echo® MS+ system and its applications in bioreactor monitoring and cell line development can provide rapid, robust, and high-throughput analysis with strong correlation to traditional LC-MS methods, enabling the analysis of crude supernatants and intact proteins.
Discover how the Echo® MS+ system can streamline cell line development with an application for small-scale bioreactor monitoring with rapid, robust, and high-throughput analysis.
Towards single cell metabolomics
- James Cox (Associate Professor of Biochemistry, University of Utah)
Single-cell metabolomics has the potential to revolutionize our understanding of metabolism at the individual cell level. By integrating advanced sample cleaneup, meticulous reagent preparation, and cutting-edge instrumentation, our workflow enables reliable metabolomic profiling from as low as several thousand cells.
Key takeaways from the presentation:
- Enhanced sample preparation improves sensitivity
- High-purity reagents minimize analytical artifacts
- Advances in instrumentation enables low-input profiling
Q&A roundtable: advanced strategies in analytical science
- Moderated by: Susan Darling
