Innovative solutions for expanded PTM and spatial analysis
Listened as expert speakers highlighted improved workflow solutions that benefit the study of post-translational modifications (PTMs), including phosphorylation and histone modifications, as well as advancements in spatial proteomics analysis using label-free quantitation strategies. These innovations are enhancing the precision and site localization of PTM analysis and spatial resolution for proteomics, providing valuable insights into cellular processes and protein functions. This session offered an in-depth look at how these improved workflows are advancing research in molecular biology and biochemistry.
Learning points:
- How to build end-to-end PTM workflows that increase coverage and site localization (phosphorylation, histone marks) via orthogonal enrichment, optimized LC, complementary fragmentation (e.g., HCD/ETD/EThcD), and rigorous localization scoring.
- How to set up label-free quantitation (LFQ) for PTM and spatial studies—using calibration/spike-ins, QC replicates, normalization, and CV tracking—to deliver reproducible, decision-ready data.
- How to choose between capture-based and direct-injection LC-MS approaches for PTM and spatial experiments, understanding trade-offs in depth, throughput, unique-protein yield, and reproducibility.
Who should attend:
- Proteomics & PTM Researchers: Scientists studying phosphorylation or histone modifications who need higher confidence in site-specific calls and robust quantitation.
- Spatial Proteomics / Spatial Biology Scientists: Teams mapping protein/PTM distributions in tissues using label-free strategies and seeking better spatial resolution with trustworthy quantitation.
- Method Developers, Analytical Chemists & Data Analysts: Professionals benchmarking workflows and building LFQ pipelines with strong QC, normalization, and reporting practices.
If you cannot attend this webinar please register to receive a link to the On Demand version the following day.
Presenter: Benjamin Garcia Ph.D. (Professor, Washington University in St. Louis)
Presenter: Emma Lundberg Ph.D. (Associate Professor, Stanford University)
Presenter: Joshua Coon Ph.D. (Professor, University of Wisconsin-Madison)
Presenter: Brett Larsen (Senior Vertical Marketing Manager, Thermo Fisher Scientific)
