HPLC Mysteries Unveiled: Solving Retention Time and Resolution Challenges

Overview

In this presentation, we will cover the following key topics:

• Fundamental principles of retention in liquid chromatography (LC)
• Understanding void time and retention factors
• Exploring the relationship between retention factor and resolution
• Strategies for addressing common challenges in retention time and resolution

Key Learning Points

• Understand how compounds are retained in LC 
• Learn the difference between retention time and retention factor, and the importance of the void time 
• Find how retention influences resolution 
• Gain insight how to influence retention and resolution to solve or prevent issues 

Who Should Attend

• Any HPLC user who is currently facing issues with retention times and/or resolution in developing or troubleshooting their method, or who want to prevent these issues later.

Speaker: Koen Askamp (Technical Specialist)

Koen Askamp is a Technical Specialist in Phenomenex. He was awarded his BSc and MSc degrees in Molecular Life Sciences at Wageningen University in the Netherlands and joined Phenomenex in 2020 as Technical Sales Consultant. Koen became part of Phenomenex’s global technical support team in 2022 and assists laboratories in training chromatographers and developing analytical methods.