Embrace simple nucleic acid desalting and quantification with Unagi and Lunatic
The Problem:
Desalting and quantifying nucleic acids is essential for ensuring accuracy and efficiency for downstream assays, but the process can be challenging. Manual methods of desalting are time-consuming and costly. Fluorescence-based methods of quantification require standards, dyes and dilutions while the accuracy of traditional UV absorbance is affected by impurities or varying sequences.
The Solution:
Unagi makes desalting a breeze. Simply load up to 8 samples of 8 mL and step away while Unagi removes solvents like ACN, salts and small molecules. End up with a purified product in any buffer, that can even be concentrated up to 16-fold.
Lunatic guarantees accurate UV/Vis quantification from just 2 μL of sample - dye and dilution free!
The Proof:
Tune in to see how simple it is to set up a desalting concentration run on Unagi and how Lunatic quickly and accurately quantifies nucleic acids with different sequences.
Learning Objectives
- Discover how buffer exchange can make desalting plug-and-play
- How to use UV/Vis to quantify DNA and RNA, dye-free
- Conclude that desalting, concentrating and quantifying nucleic acids can be done with minimal hands-on time
Presenter: Kevin Lance, PhD (Director, Product Management, Unchained Labs)
Kevin Lance directs product management and covers the Stunner portfolio globally at Unchained Labs. His expertise includes biophysical characterization of viral vectors, lipid nanoparticles, proteins, and nucleic acids. Prior to Unchained Labs, his research experience covered the fields of sustained antibody delivery and nanotechnology. He earned his Ph.D. in Bioengineering from the joint UC Berkeley – UCSF Bioengineering Graduate Program.
