Imaging Large Multiprotein Complexes Using Native Nano-DESI Mass Spectrometry
Native ambient mass spectrometry (NAMS) imaging enables visualization of the spatial distribution of proteins and their complexes in thin tissue sections. Comprehensive analysis of multiprotein complexes has been limited because previous hardware was unable to access their larger molecular weights. Moreover, the direct nature of NAMS tissue sampling results in samples that are inherently complex.
In this webinar, the benefits of the Thermo Scientific Orbitrap Ascend Structural Biology Tribrid mass spectrometer, coupled to a custom nano-DESI source, in analysis of these native complexes will be demonstrated.
You will learn:
- How the Orbitrap Ascend Structural Biology Tribrid MS can accelerate native workflows
- How to derive cellular information from native MS of protein complexes using MS
- How imaging MS combined with native structural analysis can be used to show proteoform level information
Presenter: Helen Cooper, PhD FRSC (Professor of Mass Spectrometry, University of Birmingham)
Helen Cooper undertook her BSc in Chemistry at the University of Warwick, before going on to study for her PhD under the supervision of Peter Derrick. After her PhD, she remained at the University of Warwick as technical officer is the newly created EPSRC national FT-ICR mass spectrometry facility. In 2000, she moved to the National High Magnetic Field Laboratory at Florida State University to work with Prof Alan Marshall, inventor of FT-ICR mass spectrometry. She returned to the UK in 2003 to establish an independent career at the University of Birmingham.
Helen’s research focuses on developing mass spectrometry methods for in situ analysis of proteins. She is Associate Editor of the Journal of the American Society for Mass Spectrometry. She is a Fellow of the Royal Society of Chemistry and winner of the 2022 RSC Theophilus Redwood Award for analytical chemistry.
