Under Pressure: Incorporating Column Chemistry and HPLC Theory Principles into Method Development

There are a lot of column chemistry and size options in the chromatography world. How do you know what is best for your method? The first part of this talk will cover determining which bonded phase and mobile phases to use for your analysis. In the second half, we will use HPLC theory as a backbone to discuss how parameters such as flow rate, particle size, and column dimensions affect your separation.

Presenter: Natalie Rasmussen (LC Column Applications Scientist, Agilent Technologies, Inc.)

Natalie graduated from the University of Utah with a BS in biology in 2004. She joined ARUP Laboratories in Salt Lake City in 2005. She worked in a clinical toxicology lab, preparing and analyzing meconium, urine, and serum samples by enzyme immunoassay and mass spectrometry (LC-MS/MS and LC-(Q)TOF-MS). After 8 years of routine bench work, she moved into the research and development area. There she coordinated a head injury study with the University of Utah football team, developed and improved mass spec-based diagnostic tests, collaborated in research studies, presented at national conferences, and contributed to peer-reviewed publications and application notes. She joined Agilent in 2017 as a LC Column Applications Scientist.