Advancements in Liquid Chromatography Method Development and Method Optimization

This seminar is designed to implement the principles of HPLC/UHPLC optimization to facilitate better method development. We will discuss how key features of different LC systems affect chromatography as well as software that can minimize lab work and speed discovery.

Presenter: Patrick Cronan (LC Applications Scientist, Agilent Technologies, Inc.)

Patrick Cronan is an LC applications Scientist with Agilent Technologies. He is an accomplished analytical chemist with over 15 years of experience in the pharmaceutical and Biopharma industries; has facilitated method transfer from HPLC to UHPLC while using LCMS to characterize large and small molecule drug-like compounds. Patrick has also worked extensively with Agilent’s 2D Chromatography system developing methods to increase throughput of biologic separations by coupling multiple techniques like SEC x IEX.

Presenter: Bob Giuffre (Application Scientist, Agilent Technologies, Inc.)

Bob Giuffre has been working with HP/Agilent for about thirty years as an Application Scientist for the HPLC product line. During that time Bob has worked on applications ranging from small molecule pharmaceutics, to large molecule polymers, to monoclonal antibodies. Currently, Bob is working in the area of ultra-high pressure two-dimensional liquid chromatography as a means to increase peak capacity for complex formulations. Bob has authored a chapters on the use of Diode-Array Detection in Pharmaceuticals and on Troubleshooting Peptide and Proteins Analysis using LC-MS.