A new standard for sensitivity - single cell proteomics
Traditionally, proteomics experiments are applied to large populations of cells, representing the average protein expression under given biological conditions. However, understanding cellular heterogeneity provides insights that cannot be gained from bulk studies, such that the analysis of single-cell protein expression is of growing interest. Current LC-MS-based proteomics workflows have not been widely applicable in single cell analysis, mainly due to large sample losses during sample preparation, limited analytical sensitivity and low throughput.
In this webinar we address these challenges, when we combine nanoPOTS (Nanodroplet Processing in One-pot for Trace Samples) technology with tandem mass tag (TMTTM) isobaric labeling to efficiently process and analyze single mammalian cells containing ~0.2 ng total proteins on the new Orbitrap Eclipse Tribrid Mass Spectrometers with real time search and the FAIMS Pro Interface to improve single cell proteome coverage and enhance quantification accuracy. We also focus on application of FAIMS Pro Interface to enhance label-free workflow which has resulted in a unprecedented protein/peptide coverage from a single HeLa cells with high-confidence MS2 spectra up to 3X higher than coverage without FAIMS.
Our data has demonstrated that single cell proteomes can be quantified using label-free or TMT workflows by combing nanoPOTS with the Orbitrap Eclipse Tribrid mass spectrometer, and the FAIMS Pro Interface, enabling researchers to investigate cell heterogeneity as well as rare cells in an ultra-sensitive, higher throughput LC-MS analysis.
Who should attend:
- Director, PIs & Managers of Proteomics labs
- Director, PIs & Managers of Biopharma labs
- Current users of Q Exactive technology & QTOF MS analyzing peptides & proteins
What you will learn:
- Ultra-sensitive LC-MS workflows for single cell proteomics analysis
- High throughput single cell protein quantitation without compromising on protein coverage
- Application of FAIMS Pro Interface for sample limited projects like single cell proteomics
Presenter: Khatereh Motamedchaboki, Ph.D. (Vertical Marketing Specialist, Thermo Fisher Scientific)
Khatereh is a proteomics vertical marketing scientist at Thermo Fischer Scientific, working on cutting edge technologies like Single Cell Proteomics and developing advanced proteomics workflows on new LCMS product platforms. Prior to Thermo Fisher Scientific Khatereh was the Director of the NCI- Designated Cancer Center Proteomics Core at Sanford Burnham Preby’s Medical Discovery Institute in La Jolla for 10 years building a successful proteomics core. She was a key contributor to development of BioID: A Screening method for mapping Protein‐Protein Interactions and N-Terminomics workflow for protease substrates identification. She has many scientific contributions in the field of Discovery and Targeted Proteomics, Protein PTM analysis and Protein Interactions and past industry experience in LCMS integrated solution marketing for BioPharma and metabolomics field.
