Innovative LC/MS Techniques for Oligonucleotide Analysis Without Ion Pairing Reagents
This webinar will delve into the limitations of traditional Ion Pairing Reversed Phase (IP-RP) chromatography, particularly its high costs, environmental impact, and need for specialized equipment. We will explore Hydrophilic Interaction Chromatography (HILIC) as a promising alternative, highlighting its compatibility with mass spectrometry (MS) and the absence of ion-pairing reagents, which offers greater flexibility in instrumentation.
Additionally, we’ll discuss recent advancements in ion pairing-free RP approaches for oligonucleotide analysis using LC/MS, showcasing studies on a range of oligonucleotides from 14mer to 103mer. Key parameters for optimizing LC/MS performance in these methods will also be covered.
Key Learning Objectives:
- Understanding the HILIC retention mechanism and stationary phase selection to optimize oligo resolution and MS performance
- Innovative reversed-phase separation free of ion-pairing reagents for MS characterization of nucleic acid therapeutics
Who Should Attend:
- Oligonucleotide Analytical Method Development Scientists
- Oligonucleotide Purification Method Development Scientists
- Oligonucleotide Process Development Scientists
- Oligonucleotide QC Scientists
- Oligonucleotide CDMO Labs
Presenter: Connor Flannery (Global Product Manager HPLC Bio Columns, Agilent Technologies)
Presenter: Lee Bertram (Applications Scientist, Agilent Technologies)
