An Introduction to Ion-Paired Reverse-Phase Oligonucleotide Separations: From Analysis to Purification
With the worldwide growth in the oligonucleotide markets, applying the appropriate tools for oligonucleotide analysis and purification is essential. Ion-paired reverse-phase (IP-RP) liquid chromatography involves the use of ion-pairing reagents to form neutral, hydrophobic complexes with charged oligonucleotides, facilitating their retention and separation on reversed phase columns.
This presentation introduces the principles and applications of IP-RP in oligonucleotide analysis. It will cover key factors influencing the efficiency of IP-RP methods such as column chemistry, choice of ion-pairing reagent, and mobile phase composition. Additionally, we will discuss method considerations for scaling up your oligonucleotide purification method.
Presenter: Melissa Goodlad (Application Engineer, Agilent Technologies, Inc.)
Melissa Goodlad is an Application Engineer providing applications assistance and technical support for Agilent’s HPLC columns and consumables. Before joining Agilent, Melissa was completing her PhD in Physical Chemistry at UC Merced studying nanoparticle self-assemblies.
