SEC for Ultra-large Analytes - Column Selection and Method Development for Next Generation Biotherapeutic Molecules

Next generation biotherapeutic molecules such as adeno-associated viruses (AAV), virus-like particles (VLP), oligonucleotides, and lipid nanoparticles (LNP) represent unique analytical challenges. Some unique analyses are required, but familiar techniques like Size-Exclusion Chromatography (SEC) are still useful for aggregate analysis. 

This webinar will expand upon the previous webinar, applying basic SEC theory and principles to larger analytes, while addressing what changes might be necessary for novel sample types. We’ll discuss the larger pore sizes necessary for these larger samples, selecting an SEC column, detection, and method optimization.

Presenter: Anne Blackwell, Ph.D. (Bio Columns Product Support Scientist, Agilent Technologies, Inc.)

Biography:Anne’s work in analytical chemistry began at Saint Louis University where she received Bachelor Degree in both Chemistry and French. She then began her career in mass spectrometry at the University of Arizona where she received her Ph.D. under Dr. Vicki Wysocki, studying mass spectrometry and ion mobility as tools for structural determination of non-covalent protein assemblies. She joined Agilent in 2012, working as an application scientist on LC/QTOF and LC/IM/QTOF instrumentation specializing in metabolomics and lipidomics applications. In 2016 she moved into Agilent’s bio-columns group, providing customer support for biological chromatography applications.