Single-cell proteomics and transcriptomics of human hematopoiesis reveals proteome specific features
This presentation outlines a study on small cells using a 750 milliseconds injection time via the reticle method, allowing detailed analysis of cell states and functional protein covariates. Labels were transferred between modalities, enabling comparisons without requiring a Pet set of data.
Differences in processes were identified, providing insights into important proteins not suggested by mRNA levels. A multi-omics analysis, combining transcriptomics and proteomics, was conducted using a self-created single cell transcriptomics dataset annotated with Asimov's reference mapping approach.
This allowed the identification of differentiation trajectories and heterogeneity within cell populations. However, transcriptomics alone was insufficient, as mRNA may not effectively represent protein levels. Therefore, a single cell proteomics dataset of the HSC compartment was measured from human bone marrow using mass spectrometry-based methods. TMT Pro with 14 single cells and a carrier channel was employed to enhance protein identification rates. The study identified proteins crucial for HSC maintenance, revealing insights not apparent from mRNA data, thus advancing understanding of cell state biology.
Learning points:
- Integration of Multi-Omics Data
- Advanced Analytical Techniques
- Functional Insights into Stem Cells
Who should attend:
- Researchers involved in multi-omics studies, particularly those combining transcriptomics and proteomics
- Specialists in mass spectrometry and proteomics can gain knowledge on the latest techniques and methodologies
- Scientists studying hematopoietic stem cells (HSCs) and their differentiation processes
Presenter: Benjamin Furtwängler (PhD Student, DTU, Copenhagen)
Benjamin Furtwängler is a PhD student in the Porse Group at the Copenhagen University. The group is part of the Finsen Laboratory, a department for basic cancer research, and is interested in understanding stem cells and their differentiation in hematopoiesis and leukemia.Benjamin is also supervised by Erwin Schoof, Associate Professor at the Technical University of Denmark. Erwin’s group is developing mass spectrometry-based single-cell proteomics methods and together with the Porse group, aims to apply these methods to further the understanding of stems cells in healthy and malignant hematopoiesis.
He earned his bachelor's and master's degree from the Technical University Berlin, during which time he conducted research utilizing mass spectrometry-based proteomics methods in both the Rappsilber Laboratory and the Selbach Laboratory.
