SCIEX summit Day two: Characterization and discovery

Day two: Characterization and discovery

• Characterize and quantify calreticulin (CALR) arginylation using electron-activated top-down proteomics
• Achieve in-depth profiling of metabolites and lipids in biological samples
• Obtain accurate and reliable metabolite identification using electron-based fragmentation (EAD)
• Detect trace amounts of pesticide residues in food by targeted and non-targeted, IDA and SWATH approaches
• Enhance routine characterization of low-abundant sequence variant (SV) through fast and sensitive EAD-DDA

Agenda-at-a-glance

Welcome and introduction to characterization and discovery

Top-down characterization of post-translational arginylation using electron activated dissociation (EAD)

• Learn about our top-down proteomics workflow for the characterization and quantification of calreticulin (CALR) arginylation. MS1 profile and MS2 fragmentation were characterized by EAD. Preferential fragmentation at the CALR N-terminals yielded sufficient c ions facilitating precise localization of the arginylation sites. Learn about:
  • Arginylation regulates CALR degradation and ER function
  • EAD is efficient in characterizing N-terminal arginylation
  • The workflow sets up the foundation for unbiased top-down activity-based arginylation profiling

In-depth metabolomics and lipidomics analyses

• Routine measurement of metabolites and lipids has become a cornerstone of biomedical research. At the MPC, Helmholtz Munich, non-targeted analysis is based on the SCIEX ZenoTOF 7600 for accurate and in-depth analysis and annotation of metabolites and lipids from different biological specimens.
  • Application of Zeno trap enabled non-targeted workflows to large scale metabolomics and lipidomics studies
  • Enhanced sensitivity enables deeper profiling of biological samples
  • Increased confidence in identifications through comprehensive structural elucidation using Electron activated dissociation (EAD)

MetID innovations in pharma: exploring new avenues for metabolite structure elucidation

Time-of-flight mass spectrometry applications in food science: targeted and non-targeted approaches

• Time-of-flight mass spectrometry has become a key tool in analytical laboratories involved in food analyses. This instrumentation can be used for both targeted and non-targeted approaches. In this presentation, we will discuss:
  • Overview of practical targeted and non-targeted approaches in food analysis
  • Application of a SWATH® approach to the analysis of pesticide residues in food
  • Application of IDA approaches in non-targeted food analyses

Reduce risk and accelerate cell line development with iCIEF-UV/MS technology

• Guide decisions during cell line development with the Intabio ZT system with more information on how process changes affect cell line and product stability.  
  • How to utilize icIEF-UV/MS to identify sequence variants
  • How this information can be used to create a mitigation strategy
  • What more information in less time means to groups throughout process development

Q&A roundtable: A deeper look at high-resolution mass spectrometry

• Moderated by: ose Castro-Perez, Vice President of Product Management, SCIEX
• Panelists include:
  • Tom Lin, Instructor, Washington University in St. Louis
  • Michael Witting, Deputy Head Metabolomics and Proteomics Core and Executive Manager Metabolomics, Helmholtz Munich
  • Francisco José Díaz-Galiano, Postdoctoral researcher, University of Almería
  • Kirsten Nields, Principal Scientist, J&J Innovative Medicines
  • Tatjana Talamantes, Senior Product Manager, Accurate Mass, SCIEX

Moderator: Tatjana Talamantes, Senior Product Manager, Accurate Mass, SCIEX

Tatjana is a Senior Product Manager at SCIEX, overseeing accurate mass systems since 2021. She collaborates with R&D, sales, and marketing teams to ensure effective project execution and portfolio management.

Previously, Tatjana was a Product Manager at MOBILion Systems, leading the pre-commercialization of SLIM-based ion mobility instruments. She also has held roles at Thermo Fisher Scientific as a Sr. Vertical Marketing Specialist and Sr. Field Applications Scientist.

Tatjana holds a B.A. in chemistry from Austin College and a Ph.D. in biomedical sciences and biochemistry from the University of North Texas Health Science Center.

Jamie holds a B.Sc. in engineering physics from Queen’s University.

Presenter: Tom Lin, Instructor, Washington University in St. Louis

Presenter: Michael Witting, Deputy Head Metabolomics and Proteomics Core and Executive Manager Metabolomics, Helmholtz Munich

Presenter: Francisco José Díaz-Galiano, Postdoctoral researcher, University of Almería

Presenter: Kirsten Nields, Principal Scientist, J&J Innovative Medicines

Presenter: Tatjana Talamantes, Senior Product Manager, Accurate Mass, SCIEX