SCIEX summit Day one: Innovation and data insights

Day one: Innovation and data insights

• Discover how to achieve high sensitivity, speed, and resilience when working with large, complex sample sets
• Learn strategies to speed up your covalent protein drug discovery
• Gain insights into accelerating the development of multiple modalities
• Understand the latest advancements in quantitative proteomics with ZT Scan DIA
• Overcome limitations with cutting-edge artificial intelligence quantitation software

Agenda-at-a-glance

Welcome and introduction to innovation and data insights    

Advancing robustness and serviceability of a high-end triple quad mass spectrometer in a high-throughput bioanalytical lab

• Highly sensitive mass spectrometers allow the advanced bioanalytical labs of today to achieve the LOQ required of potent biologic drugs and provide maximum flexibility in choice of sample prep techniques. However robust mass spectrometers are crucial for several reasons:
  • Aid in ensuring the consistent quantitative performance of an assay
  • Effectively allow a lab to meet their project timelines
  • Are essential for handling the inherent variability of analyzing diverse molecules and diverse samples required for bioanalysis

The new fast in drug discovery: a new MS system to study covalent protein drugs

• The combination of a super-fast sampling interface with the highly sensitive ZenoTOF 7600 system makes it an ideal system for evaluating covalent binding screens. Main findings in our experiments using the Echo® MS+ system:
  • Speed: measurements from a full 384-well plate in 15 min or 1536-well plate in less than one hour
  • Precision: low injection volume supports repetitive measurements and time series; capture full kinetic data set in one experiment
  • Quality: high data quality allows for full automated processing, delivering immediate hitlist output

icIEF-UV/MS characterization of charge Isoforms for biotherapeutic products

• Characterization of icIEF charge isoforms, whether in early product development or associated with licensure filings, is usually achieved through ion exchange (IEX) liquid chromatography coupled with mass spectrometry. icIEF-MS technology has recently emerged to enable the direct characterization of specific species in the icIEF electropherogram, eliminating the need for extensive method development.
  • Discover opportunities to advance core platform technologies with novel characterization approaches
  • Hear case studies on understanding molecular structure information
  • New approaches to characterizing post-translational modifications of charge isoforms

Expanding qualitative and quantitative sensitivity for immunopeptidomics using ZT Scan DIA

• Learn how utilizing a research-grade Zeno-trap time-of-flight LC-MS system can advance current applications in immunopeptidomics and proteomics, including:
  • What is ZT Scan DIA, the technology and how it benefits data acquisition
  • Preliminary proteomics and immunopeptidomics results using ZT Scan DIA
  • Future applications of immunopeptidomics using ZT Scan DIA

Artificial intelligence quantitation (AI quant): molecular structure and mass spectral data quality driven processing of high-resolution mass spectrometry for quantitative analysi

• High-resolution mass spectrometry (HRMS) has long sought to be a viable alternative for quantitative workflows but has been unable to broadly compete, mainly due to the lack of suitable data processing software. In this work we describe:
  • A molecular structure-dependent workflow
  • The holistic and user-independent processesing of HRMS data
  • The automatic quantification of small drug-like molecules

Q&A roundtable: innovation and data insights with the experts

• Moderated by: Jamie Wighton, Senior Director LC-MS Research, SCIEX
• Panelists include:
  • Rathna Veeramachaneni, Director, Biopharma LC-MS/MS, KCAS Bio
  • Xiaping He, Senior Scientist, Pfizer
  • Markus Stöckli, Senior Principal Scientist, Novartis Institutes for BioMedical Research
  • Tony Purcell, Professor, Monash University 
  • Kevin Bateman, Retired Pharmacokinetics, Pharmacodynamics and Drug Metabolism Expert

Moderator: Jamie Wighton (Senior Director LC-MS Research, SCIEX)

Jamie joined SCIEX 20 years ago and has held multiple technical and leadership roles throughout his career at SCIEX.  Starting out as an engineer in manufacturing operations, Jamie has since led teams in supply chain, product development, and most recently the research and product applications teams within R&D. Prior to joining SCIEX, Jamie worked at JDS Uniphase as an optical test engineer.

Jamie holds a B.Sc. in engineering physics from Queen’s University.

Presenter: Rathna Veeramachaneni (Director, Biopharma LC-MS/MS, KCAS Bioanalytical)

Presenter: Markus Stöckli, Senior Principal Scientist, Novartis Institutes for BioMedical Research

Presenter: Xiaoping He, Senior Scientst, Pfizer

Presenter: Tony Purcell, Professor, Monash University

Presenter: Kevin Bateman, Retired Pharmacokinetics, Pharmacodynamics and Drug Metabolism Expert