mRNA Characterization - From 5’ Cap to Poly (A): What IPRP–MS Can Tell You

Event Overview:

In this webinar, we present an mRNA characterization workflow that includes sample preparation, LC-MS methodology and bioinformatics processing for the evaluation of 5' Cap efficiency, mapping of oligonucleotide sequence fragments, isomer resolution and Poly (A) tail intact mass analysis.

Who Should Attend:

• Researchers and Scientists: Ideal for those seeking to expand their insights into LC-MS characterization techniques and applications of mRNA modifications.
• Quality Control and In-Process Testing Professionals: Recommended for professionals involved in quality control, in-process testing, or analytical development.
• Biopharmaceutical Industry Professionals: Valuable for those evaluating and optimizing their current mRNA-based therapeutics development and characterization solutions.

Key Learning Points:

• Explore the LC-MS characterization method for 5' Cap, coding sequence mapping, and Poly(A) tail analysis.
• Gain insights into utilizing ion-pairing reversed-phase LC-MS to characterize mRNA drug substances.
• Discover the benefits of utilizing BioTi core-shell column technology to improve sensitivity and resolution for oligonucleotide identification.
• Review of bioinformatics processing workflow

Presenter: Roxana Eggleston-Rangel (Senior Applications Scientist, Phenomenex)