Analyzing High Molecular Weight (HMW) DNA Size Distribution for PacBio HiFi Long Read Sequencing Services using Agilent Femto Pulse System
Next-generation sequencing (NGS) technologies enable deeper insights into different areas of research. In this new webinar series, researchers from Psomagen, Decode Health, and Agilent Technologies discuss how quality control steps in PacBio Hi Fi long-read sequencing and RNA sequencing support the discovery of new biomarker candidates for diagnostic and therapeutic applications.
Assessing the quality of genomic DNA (gDNA) is of utmost importance in next-generation sequencing (NGS) workflows. Recently, there has been a growing interest in long-read sequencing, as it offers researchers the capability to analyze genomes more comprehensively and accurately. Traditionally, agarose gel electrophoresis/pulsed field gel electrophoresis (PFGE) has been the preferred quality assessment method for long-read sequencing. However, these techniques have limitations regarding sample usage and longer run times. Conversely, recent technological advancements have introduced instruments like the Agilent Femto Pulse system. These instruments can accurately characterize the size distribution of high molecular weight (HMW) DNA and SMRTbell libraries with faster run times and minimal DNA requirements, typically only picogram amounts. This webinar aims to provide an overview of the applications and key benefits of the Femto Pulse system, particularly its use in PacBio long-read sequencing workflows.
Key learning objectives
- Explore how to effectively assess the quality of genomic DNA (gDNA) in NGS workflows
- Learn how the Agilent Femto Pulse system is used in PacBio long-read sequencing workflows
- Find out how to determine the number of moles of a sample to better predict long-read sequencing read length
Presenter: Abhilasha Cheruku, MS (Product Manager-Genomic Services, Psomagen, Inc., United States)
Presenter: Dr. Leslie Friedmann (Product Manager for Parallel CE instruments, Agilent Technologies, Germany)
