Practical Approaches to Preparative Chromatography

When creating a Preparative Chromatography method, the concerns is the quantity of the crude material, its impurities, and the resources that are available. Preparative separations become an economic decision as we balance the cost of solvents, adsorbents and time. What is most important will weigh in deciding which column and method we want to use.

In this presentation the focus will be on three of the most common purification challenges today; that of peptides, oligonucleotides, and the high throughput small molecules. Whether you have a lot of one compound to purify or small amounts of many compounds to purify, different tactical approaches will be discussed as to how to achieve your purification objectives.

Presenter: Carl Griffin (LC Column Applications Scientist, Agilent Technologies, Inc.)

Carl Griffin started his career as an Organic Chemist working at the Kilo scale in a pharmaceutical company where he got his first exposure to preparative chromatography. Before joining Agilent in 2010, he worked with manufacturers of preparative and process chromatography instrumentation and media including Polymer Labs (now apart of Agilent). He has worked on projects from mgs to kgs, from small molecules to the largest biomolecules.

Presenter: Paul Dinsmoor (Large Molecule Applications Scientist, Agilent Technologies, Inc.)

Paul Dinsmoor is a Large Molecule Field Applications Scientist with Agilent Technologies in La Jolla, CA. He has a Biochemistry & Cell Biology degree from UC San Diego with over 30 years of bio chromatography experience which includes 15 years as a bioanalytical chemist in research and development at La Jolla Pharmaceutical & Pfizer as well as 14 years as a field application scientist at Polymer Labs/ Varian/ Agilent focusing on BioHPLC analysis and macromolecular characterization. At Agilent Paul helps customers with column recommendations, method development, and troubleshooting.