Scaling Up Analytical Methods to HPLC; A Story of Risk Mitigation Across Workflows using MaxPeak Premier Columns

Risk mitigation strategies are vital to the operation of any laboratory. Reducing the amount of rework, or the potential for downtime due to poor performing analyses is critical. One way to ensure a successful liquid chromatography analysis is to use technology that is specifically designed to reduce the risk of unexpected or unwanted interactions between analyte and system hardware.

MaxPeak Premier Columns are designed specifically to reduce and eliminate the risk of unwanted interactions between metal hardware, such as column tubing, and the analytes of interest in an assay. Having gained a lot of interest in laboratories utilizing UPLC instruments and/or sub-2 µm particle size columns, this technology has gone a long way to improve analyses, but was inaccessible to laboratories using older technologies, such as HPLC systems. Recently this hardware has become available in various HPLC configurations using 3.5 µm particle size columns, allowing more laboratories to take advantage of this technology.

For some laboratories, the use of MaxPeak Premier HPLC Columns is as easy as integrating them into method development workflows. Others, however, may be limited by the source of an analytical method, such as a design and discovery group that transfers an impurity assay to a QC group. In those cases, it is important to not only match the technology in the original method but also scale the method to appropriate column technology, suitable for the instruments available. This webinar will briefly introduce what MaxPeak High Performance Surface (HPS) Technology is and how it benefits LC analyses. Additionally, examples will be given on how to properly scale a method from smaller particle sizes, often used in discovery workflows, to larger particle sizes which are more common in QC laboratories. One case study of such a scale-up will be shown moving a pre-existing UPLC method to two different HPLC systems using the MaxPeak Premier HPLC Columns and the various tools that can make such an activity a success. By matching column technology across different workflows, the risk of something going wrong is reduced, leading to greater success in sample analysis.

Learning Objectives:

Attendees will learn

1. What MaxPeak Premier Column hardware is and how it can improve LC separations
2. How to scale an analytical method between particle sizes
3. The importance of matching system dwell volume for gradient systems when scaling
4. How to account for system dwell differences when scaling methods

Who Should Attend

• Analytical scientists who routinely develop HPLC methods
• Analytical scientists responsible for integrating pre-existing methods into their lab by scaling from original source material (i.e. USP monographs, journal articles)
• Analytical scientists who transfer their developed methods to other labs that may use older technology
• Lab managers interested in reducing risk in their laboratory
• Lab managers who want to understand the latest technologies with the potential of improving their current workflows

Presenter: Kenneth Berthelette (Senior Scientist, Waters Corporation Chemistry and Consumables Group)

Ken earned his Bachelor's of Science degree in Professional Chemistry from Bridgewater State University, Bridgewater Massachusetts, in 2010. He has been with Waters Corporation since March 2012 and has worked on several product launches including CORTECS columns, UPC2 Torus and Trefoil columns, and the Atlantis Premier BEH Z-HILIC column. He changed job functions in 2021 and now supports Chemistry Aftermarket initiatives. His focus on applications and customer education has led to several seminars and webinars on topics including method development, method modernization and transfer, HILIC separation techniques, and LC system troubleshooting.