Waters Journal Club: A Cyclic Ion Mobility-Mass Spectrometry System

This interactive session will bring together the MS community to discuss some of the most popular and exciting peer-reviewed publications in a friendly and open forum.

Ahead of each meeting, we will send out a link to the discussion article, then during the session an expert will sumarise the main findings and a panel of experts will be on hand to answer all of your questions.

We kick off the journal with a throwback to the Cyclic IMS seminal paper. Take this opportunity to get deep into the science behind the SELECT SERIES Cyclic IMS.

Be Prepared make sure you read A Cyclic Ion Mobility-Mass Spectrometry System | Analytical Chemistry (acs.org)

Cyclic Ion Mobility is an increasingly popular approach for the structural analysis of ionic species as well as the separation of complex mixtures and is now being used to enhance more routine workflows.

Reasons to Attend:

• Debate some of the most topical papers from all-time favourites to some of the latest releases
• Join an online community with a passion for science
• Gain a greater understanding of applications and technologies by asking questions of the authors and/or expert panel.

ABSTRACT: Improvements in the performance and availability of commercial instrumentation have made ion mobility-mass spectrometry (IM-MS) an increasingly popular approach for the structural analysis of ionic species as well as for separation of complex mixtures. Here, a new research instrument is presented which enables complex experiments, extending the current scope of IM technology. The instrument is based on a Waters SYNAPT G2-Si IM-MS platform, with the IM separation region modified to accept a cyclic ion mobility (cIM) device. The cIM region consists of a 98 cm path length, closed-loop traveling wave (TW)-enabled IM separator positioned orthogonally to the main ion optical axis. A key part of this geometry and its flexibility is the interface between the ion optical axis and the cIM, where a planar array of electrodes provides control over the TW direction and subsequent ion motion. On either side of the array, there are ion guides used for injection, ejection, storage, and activation of ions. In addition to single and multipass separations around the cIM, providing selectable mobility resolution, the instrument design and control software enable a range of “multifunction” experiments such as mobility selection, activation, storage, IMSn, and importantly custom combinations of these functions. Here, the design and performance of the cIM-MS instrument is highlighted, with a mobility resolving power of approximately 750 demonstrated for 100 passes around the cIM device using a reverse sequence peptide pair. The multifunction capabilities are demonstrated through analysis of three isomeric pentasaccharide species and the small protein ubiquit.

Presenter: Kevin Giles, Ph.D (Scientific Fellow, Research and Development, Waters Corporation)

Kevin is a Scientific Fellow at Waters Corporation where he has worked for 21 years in the Mass Spectrometry research group. His primary interests have been in the development of ion guiding technology and ion mobility separators to enhance the performance and functionality of MS instruments.

Presenter: Dale Cooper-Shepherd (Principal Scientist, Waters Corporation, UK)

Dale Cooper-Shepherd undertook PhD studies at the University of Leeds and conducted postdoctoral research at the University of Oxford, UK. Throughout his training, Cooper-Shepherd specialized in applying MS techniques in the field of structural biology. He joined Waters in 2015 and since 2019 has been working on the SELECT SERIES of instruments.

Presenter: Emma Marsden-Edwards (Discovery Portfolio Director, Waters Corporation)

Emma Marsden-Edwards is Director of the MS Discovery Program at Waters. She leads a multidisciplinary team, responsible for delivering high impact applications and scientific content covering the SELECT SERIES and SYNAPT Mass Spectrometry product lines.

Presenter: Jakub Ujma (Principal Research Scientist, Waters Corporation)

Jakub Ujma is a Research Principal Scientist at Waters Corporation, where he has been working for 7 years in the Mass Spectrometry research group. He completed his PhD studies at the University of Manchester. His primary interests lie in developing novel instruments and applying them to the structural analysis of ionic species, as well as separating complex mixtures.