Analysis of Amino Acid Analysis for Food Protein Hydrolysates and Cell Culture Media

Accelerating Routine Food & Beverage Testing with Optimized Methods

Explore the latest technology and methodology solutions to help you deliver results more easily and efficiently for routine Food and Beverage QC testing for : Sugars, Sugar Substitutes Additives, Vitamins and Amino Acids.

EPISODE 4 - Amino Acid Analysis

Amino acid testing is a high-volume assay for many food and dietary supplement companies, and is needed for infant formula, animal feed, sports nutrition products, and dietary supplement labeling. The Waters AccQ-Tag™ Ultra Derivatization kit is the industry standard for amino acids analysis and is included in AOAC Official Method 2018.16 for infant formulas, adult nutrional's, dairy and cereals. While kits make analyses easier, it is important to consider which sample preparation tecniques and LC systems are best fit for your specific needs

This presentation will include:

• An overview of the Waters AccQ-Tag Ultra Derivatization kit for amino acid analysis

• Sample preparation for free and bound amino acids in various matrices

• HPLC and UPLC™ options for separation and detection

• Increased workflow efficiency with the Andrew+Pipetting Robot

Guest Speaker Presentation summary:

Amino acids are key nutrients and building blocks for all proteins. They need to be provided in adequate quantities through food to ensure proper growth and to maintain good health. Amino acid profiling helps us ensure the high quality of products such as infant formulas, nutritional products, dairy products, and cereals. A standardized method is needed to ensure consistent and comparable results across the many different labs that perform amino acid profiling. In this presentation, I will share the standardization journey of our total amino acid method based on pre-column derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), and discuss next steps to improve method throughput.

Live Q&A session

Moderator: Cecile Pinto (Food & Environmental Market Development Manager, South Europe - Waters)

Presenter: Christophe Fuerer (Senior Specialist Proteins Analysis, mass spectrometry, analytical chemistry & Proteins, Société des Produits Nestlé SA)

Manage the Nestlé portfolio of analytical protein methods, evaluate proficiency tests, develop andvalidate analytical methods, liaise with standardization bodies, provide expert assistance to qualityassurance centers, R&D centers, and factories, supervise lab technicians, and coordinate lab work.

• Mass Spectrometry
• Intact protein analyses (proteoform fingerprinting and quantification) by UHPLC-HRMS, with anemphasis on the detection of genetic variants and protein glycation
• Multiple Reaction Monitoring (MRM) methods for absolute quantification of trace allergens infood matrices and milk proteins in dairy matrices
• Method development and validation
• Intact protein fingerprinting, detection, and quantification of milk proteins by UHPLC-HRMS
• Amino acids in food matrices by UHPLC-UV
• Whey protein content by capillary gel electrophoresis
• Trace amounts of intact proteins by gel electrophoresis
• Method standardization and active contribution to standardization bodies
• AOAC International
• Expert Review Panel on Nutrients (2022-present, member)
• Total Amino Acids by UHPLC-UV (Final Action 2018.06, author)
• Whey protein content in infant formula by SDS-CGE (Final Action 2016.15, author)
• A1- and A2-type beta casein (SMPR 2022.005), Lactoferrin (SMPR 2020.005), and Food allergens by MS (SMPR 2016.002) (working group member)
• IDF/ISO
• Total Amino Acids by UHPLC-UV (IDF 254, ISO 4214, author)
• Whey protein content by SDS-CGE (IDF 247, ISO 23293, author)
• Evaluation of nitrogen conversion factors (IDF Bulletin, co-author)
• USP
• Expert Panel on Dietary Proteins (2018-present, member)
• Detection of foreign proteins in whey (FCC, Appendix XVI, author)
• CEN
• TC454/WG7 - Algae and algae products, Product test methods - saccharides, proteins,amino-acids (working group member)

Presenter: Kimberly Martin (Evaluation Scientist II, Waters Corporation)

Kimberly Martin is part of the QA/QC Strategy Group at Waters Corporation and serves as an Evaluation Scientist II that verifies, validates and tests new instrumentation and products. She has been with the company for 5 years. She has a total of 14 years of analytical chemistry experience including polymer chemistry, food and environmental chemistry, and pharmaceutical chemistry. Kimberly has extensive experience in HPLC, LCMS, GCMS, GC-FID, additional MS technologies and sample preparation.

Presenter: Don Trinite (Senior Principal Technical Support Specialist, Waters Corporation)

Don Trinite has been working at Waters Corporation for thirty-five years. Don is a Senior Principal Technical Support Specialist for Waters Technologies Corporation for the last twenty-nine years. He is involved in developing seminars for training users in methods development and methods transfer and troubleshooting for HPLC, UPLC, IC, GPC, Prep LC and UPC² ™. He is a key person in amino acid applications development and support. Recently, he has been involved with theAndrew+™ Robotic system, testing protocols for various amino acid sample protocols to automate different aspects of sample handling for these types of samples. He is also involved in troubleshooting for Data Handling and Networking Systems. Don has also developed methods for users and has had several application notes published. Prior to this, Don was in the field service organization for Waters as Senior Service Specialist and Service Manager for five years. He was previously employed in the chemical industry by UOP, Des Plaines, Illinois, in a chromatography service group supporting chemical synthesis, production and natural product research as a Senior Research Technician. Don has a total of 43 years of experience in the LC field.